Journal: Molecular and Clinical Oncology

Article Title: Fyn expression is associated with the response of patients with locally advanced uterine cervical squamous cell carcinoma to neoadjuvant chemotherapy

doi: 10.3892/mco.2022.2580

Figure Lengend Snippet: Immunohistochemical staining of Fyn in uterine cervical squamous cell carcinoma specimens counterstained with hematoxylin. Representative images of a weighted score of (A) 0, (B) 6, and (C) 12. Scale bar, 50 µm.

Article Snippet: TaqMan chemistry was used to perform RT-qPCR according to the manufacturer's protocol with TaqMan primer and probes for Fyn (cat. no. Hs00941613_m1) and hypoxanthine phosphoribosyl-transferase 1 (cat. no. Hs02800695_ml, Thermo Fisher Scientific, Inc.) which was used as an internal control ( ).

Techniques: Immunohistochemical staining, Staining

Journal: Molecular and Clinical Oncology

Article Title: Fyn expression is associated with the response of patients with locally advanced uterine cervical squamous cell carcinoma to neoadjuvant chemotherapy

doi: 10.3892/mco.2022.2580

Figure Lengend Snippet: Analysis of the weighted score of Fyn. (A) Comparison of the weighted score of Fyn between the NAC successful group and the NAC failure group. The score in the NAC successful group was significantly lower than that in the NAC failure group (P=0.003). (B) Receiver operator characteristic curve for determining the Fyn cutoff score for predicting the effectiveness of NAC, indicating a cut off value of 3 with a sensitivity of 82.6% and specificity of 71.4% was optimal, with an area under the curve value of 0.759 and a 95% confidence interval of 0.613-0.905.

Article Snippet: TaqMan chemistry was used to perform RT-qPCR according to the manufacturer's protocol with TaqMan primer and probes for Fyn (cat. no. Hs00941613_m1) and hypoxanthine phosphoribosyl-transferase 1 (cat. no. Hs02800695_ml, Thermo Fisher Scientific, Inc.) which was used as an internal control ( ).

Techniques: Comparison

Journal: Molecular and Clinical Oncology

Article Title: Fyn expression is associated with the response of patients with locally advanced uterine cervical squamous cell carcinoma to neoadjuvant chemotherapy

doi: 10.3892/mco.2022.2580

Figure Lengend Snippet: Association between Fyn expression and NAC efficacy.

Article Snippet: TaqMan chemistry was used to perform RT-qPCR according to the manufacturer's protocol with TaqMan primer and probes for Fyn (cat. no. Hs00941613_m1) and hypoxanthine phosphoribosyl-transferase 1 (cat. no. Hs02800695_ml, Thermo Fisher Scientific, Inc.) which was used as an internal control ( ).

Techniques: Expressing

Journal: Molecular and Clinical Oncology

Article Title: Fyn expression is associated with the response of patients with locally advanced uterine cervical squamous cell carcinoma to neoadjuvant chemotherapy

doi: 10.3892/mco.2022.2580

Figure Lengend Snippet: Effect of Fyn knockdown on the sensitivity to cisplatin in vitro . (A) Relative Fyn mRNA expression levels in the control and Fyn knockdown cells. After transfection of si-Fyn, Fyn mRNA expression levels were significantly lower than that in the control cells (P<0.05). (B) Comparison of cell viability of uterine cervical cancer cells between the control siRNA group and Fyn siRNA group after treating cells with several different concentrations of cisplatin. The cell viability of the si-Fyn group was significantly lower than that of the control group when treated with 10, 25, or 50 µM cisplatin. Data are presented as the mean ± SD. * P<0.05. siRNA, small interfering RNA.

Article Snippet: TaqMan chemistry was used to perform RT-qPCR according to the manufacturer's protocol with TaqMan primer and probes for Fyn (cat. no. Hs00941613_m1) and hypoxanthine phosphoribosyl-transferase 1 (cat. no. Hs02800695_ml, Thermo Fisher Scientific, Inc.) which was used as an internal control ( ).

Techniques: Knockdown, In Vitro, Expressing, Control, Transfection, Comparison, Small Interfering RNA

Journal: Virology Journal

Article Title: Activation of Src, Fyn and Yes non-receptor tyrosine kinases in keratinocytes expressing human papillomavirus (HPV) type 16 E7 oncoprotein

doi: 10.1186/1743-422X-10-79

Figure Lengend Snippet: Presence of HPV 16 E6 and E7 has different effect on SFK protein and mRNA expression in proliferating human keratinocytes. ( A ) Western blot analysis of human foreskin keratinocytes (HFK) transduced with HPV 16 E6, E7 or both oncogenes. Levels of cellular p53 and Rb proteins are reduced in the presence of functionally active HPV 16 E6 and E7 oncoproteins, respectively. ( B ) Analysis of Src, Yes, and Fyn protein expression by western blot using antibodies specific for the native form of the kinases and for a C-terminal tyrosine residue that is phosphorylated upon activation. Actin was used as loading control. The results shown are representative of three independent experiments. ( C ) The effect of HPV 16 oncogenes on the relative mRNA expression of ubiquitously expressed SFKs in HFK cells. Real-time PCR analysis of Src, Yes, and Fyn was performed using RNA purified from HPV 16 E6 and/or E7 transduced HFK cells. The values were normalized against GAPDH mRNA and presented as relative levels with respect to the value of cells transduced by LXSN. The bars represent the mean ± SEM of two independent experiments, each with triplicate measurements. Two-tailed Student t test revealed no significant, HPV related alteration in any of the tested SFK mRNA levels.

Article Snippet: The applied TaqMan Gene Expression Assays were for Src (SRC; Hs01082246_m1), Yes (YES; Hs01080041_g1), Fyn (FYN; Hs00941600_m1), Hck (HCK; Hs00176654_m1), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 0711024) as endogenous control.

Techniques: Expressing, Western Blot, Transduction, Residue, Activation Assay, Control, Real-time Polymerase Chain Reaction, Purification, Two Tailed Test

Journal: Virology Journal

Article Title: Activation of Src, Fyn and Yes non-receptor tyrosine kinases in keratinocytes expressing human papillomavirus (HPV) type 16 E7 oncoprotein

doi: 10.1186/1743-422X-10-79

Figure Lengend Snippet: Differentiation of human keratinocytes influences SFK protein and mRNA expression in transduced HFK cells. ( A ) Western blot analysis of differentiating human foreskin keratinocytes (HFK) transduced with HPV 16 E6, E7 or both oncogenes. Cellular differentiation was induced by culturing cells in the presence of serum and high calcium. Levels of cellular p53 and Rb proteins are reduced in the presence of functionally active HPV 16 E6 and E7 oncoproteins, respectively. ( B ) Analysis of Src, Yes, and Fyn protein expression by western blot using antibodies specific for the native form of the kinases and for a C-terminal tyrosine residue that is phosphorylated upon activation. Actin was used as loading control. The results shown are representative of three independent experiments. ( C ) The effect of HPV 16 oncogenes on the relative mRNA expression of Src, Yes, and Fyn in differentiating HFK cells. Real-time PCR analysis was performed using RNA purified from the transduced HFK cells. The values were normalized against GAPDH mRNA and presented as relative levels with respect to the value of cells transduced by LXSN. The bars represent the mean ± SEM of two independent experiments, each with triplicate measurements. Two-tailed Student t-test was carried out for the analysis of mRNA expression levels to determine statistical significance (P < 0.05).

Article Snippet: The applied TaqMan Gene Expression Assays were for Src (SRC; Hs01082246_m1), Yes (YES; Hs01080041_g1), Fyn (FYN; Hs00941600_m1), Hck (HCK; Hs00176654_m1), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 0711024) as endogenous control.

Techniques: Expressing, Western Blot, Transduction, Cell Differentiation, Residue, Activation Assay, Control, Real-time Polymerase Chain Reaction, Purification, Two Tailed Test