Journal: Frontiers in Immunology

Article Title: The Procoagulant Activity of Apoptotic Cells Is Mediated by Interaction with Factor XII

doi: 10.3389/fimmu.2017.01188

Figure Lengend Snippet: Apoptotic cell-mediated procoagulant activity and intrinsic tenase formation is dependent on FXII activation. (A) Tissue factor (TF) expression as determined by flow cytometry. CEM cells treated with (apoptotic cells) or without (viable cells) 10 µM dexamethasone for 24 h were stained with CD142-PE to analyze TF expression on the cell surface. THP-1 cells were used as a positive control. The background staining with an isotype control is shown in black. (B) After viable cells and apoptotic cells (2 × 10 5 ) were suspended in 150 µL of platelet-poor plasma, clotting was triggered by the addition of 20 mM CaCl 2 and measured as described in the Section “ ” ( n = 3). *** p < 0.001. (C) After apoptotic cells (2 × 10 5 ) were suspended in normal plasma (normal), FXII-deficient plasma [FXII(-)] and FXII-deficient plasma supplemented with 375 nM FXII [FXII(-) + FXII], respectively, clotting was triggered by the addition of 20 mM CaCl 2 and measured as described in the Section “ ” ( n = 3). (D) Effect of apoptotic cells on intrinsic tenase complex formation. As indicated, apoptotic or viable cells were incubated with FXII, prekallikrein, high molecular weight kininogen, FXI, FIX, and FVIII. Then FX was added, and tenase complex formation was analyzed using a chromogenic substrate ( n = 3). Some samples were also treated with 2 µM corn trypsin inhibitor (CTI). *** p < 0.001.

Article Snippet: Human FXII, PK, HK, FIX, FX, and FXI were purchased from Enzyme Research Laboratories (South Bend, IN, USA).

Techniques: Activity Assay, Activation Assay, Expressing, Flow Cytometry, Staining, Positive Control, Control, Clinical Proteomics, Coagulation, Incubation, High Molecular Weight

Journal: TH Open: Companion Journal to Thrombosis and Haemostasis

Article Title: Modelization of Blood-Borne Hypercoagulability in Myeloma: A Tissue-Factor-Bearing Microparticle-Driven Process

doi: 10.1055/s-0039-1700885

Figure Lengend Snippet: Effect of MPCs (1,000 cells/μL) or MPC-dMPs on thrombin generation in normal PPP and in PPP depleted of FVII or FXII

Article Snippet: Samples of fresh frozen normal platelet poor plasma (PPP; Ref 00539) and immunodepleted lyophilized plasma deficient of clotting factor VII (FVII) or FXII were purchased from Stago (Gennevilliers, France).

Techniques: