Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Dietary Stearic Acid Accelerates Intestinal Tumorigenesis via Fatty Acid-binding Protein 5 Without Promoting Obesity

doi: 10.1016/j.jcmgh.2026.101740

Figure Lengend Snippet: An SA-HFD promotes Paneth cell differentiation and epithelial cell proliferation and turnover. ( A ) Representative fluorescent images of the jejunum from Lgr5-EGFP reporter mice ( upper left panels ) fed the CD, LA-HFD, or SA-HFD for 2 weeks are shown. The number of EGFP+ cells per crypt is quantified in the upper right panel . Representative fluorescent images of the jejunum from Bmi1-GFP reporter mice ( middle left panels ) fed the CD, LA-HFD, or SA-HFD for 2 weeks are also shown, with quantification of GFP+ cells per crypt in the middle right panel . In the left lower panels , formalin-fixed, paraffin-embedded jejunal sections from C57BL/6 mice fed the CD, LA-HFD, or SA-HFD for 2 weeks were stained with anti-lysozyme C. Positive areas per crypt were quantified using ImageJ. The data represent the average counts in 8 to 15 crypts from individual mice (n = 4–7). The scale bars represent 10 μm. ( B ) Relative mRNA expression of intestinal stem cell marker genes ( upper ) and Wnt target genes ( lower ) in epithelial cells isolated from the jejunum of C57BL/6 mice fed the CD, LA-HFD, or SA-HFD for 2 weeks (each group, n = 8). ( C ) Representative fluorescent images of jejunal sections stained with anti-mucin 2 ( upper left panels ), anti-Chr-A ( middle left panels ), or anti-Dclk1 ( lower left panels ) are presented. Jejunal tissues were collected from C57BL/6 mice fed the CD, LA-HFD, or SA-HFD for 2 weeks. The numbers of mucin 2+ cells ( upper right panel ) and Dclk1+ cells ( lower right panel ), and the area of Chr-A+ cells ( middle right panel ) per crypt were quantified (n = 4). Scale bars represent 50 μm. ( D ) Representative images of formalin-fixed, paraffin-embedded sections of the jejunum stained with anti-Ki67. The jejunum was obtained from C57BL/6 mice fed the CD, LA-HFD, or SA-HFD for 2 days to 4 weeks ( left panels ). The number of Ki67+ cells per crypt were counted ( right ). The data represent the average counts in 8 to 15 crypts from individual mice (n = 4). The scale bars represent 20 μm. ( E ) Representative fluorescent images of jejunal sections stained with anti-Ki67 ( left panels ). The jejunum was obtained from female C57BL/6 mice fed the CD, LA-HFD, or SA-HFD for 2 weeks. The number of Ki67+ cells per crypt were counted ( right panels ; n = 5). Scale bars represent 20 μm. ( F ) Representative fluorescent images of colon sections stained with anti-Ki67 ( left panels ). The colon was obtained from C57BL/6 mice fed the CD, LA-HFD, or SA-HFD for 2 weeks. The number of Ki67+ cells per crypt were counted ( right panels ; n = 4). Scale bars represent 20 μm. ( G ) Representative fluorescent images of jejunal sections stained with anti-Ki67 ( left panels ). The jejunum was obtained from 50-week-old C57BL/6 mice fed the CD, LA-HFD, or SA-HFD for 2 weeks. The number of Ki67+ cells per crypt was counted ( right panels ; n = 4). Scale bars represent 20 μm. ( H ) Representative fluorescent images of the jejunum from mice intraperitoneally injected with BrdU ( green ) 48 hours before dissection followed by EdU ( red ) administration 18 hours before dissection ( left panels ). Cell migration rates were assessed by the distance between BrdU-labeled cells and EdU-labeled cells. The data represent the average length of 15 to 20 villi from individual mice divided by the interval between injections (each group; n = 4). The scale bars represent 100 μm. The data represent the mean ± SEM. ∗ P < .05; ∗∗ P < .01; ∗∗∗ P < .001.

Article Snippet: The primary antibodies used in this study included Ki67 (GeneTex, #GTX16667), Lysozyme C (Santa Cruz, #sc-27958), FABP5 (R&D System, #AF1476), Chr-A (Santa Cruz, #sc-393941), Mucin-2 (SantaCruz, #sc-15334), DCAMKL1 (Abgent, #Ap7219b), CD44 (Biolegend, #103002), L-FABP (SantaCruz, #sc-374537), intestinal FABP (MyBioSource, #MBS178443), FABP6 (Proteintech, #13781-1-AP), Fgfbp1 (Proteintech, #25006-1-AP), and Msi-1 (eBioscience, #14-9896-82).

Techniques: Cell Differentiation, Formalin-fixed Paraffin-Embedded, Staining, Expressing, Marker, Isolation, Injection, Dissection, Migration, Labeling

Journal: Sports Medicine and Health Science

Article Title: Exercise preconditioning prevents immobilization-induced skeletal muscle atrophy by activating Prmt1-p38/ATF2-Sesn1 signaling axis in C57BL/6J mice

doi: 10.1016/j.smhs.2025.04.001

Figure Lengend Snippet: Exercise preconditioning prevented muscle atrophy through protein arginine methyltransferase 1 (Prmt1)-Sestrin1 (Sesn1)-transcriptional co-activator PPAR-γ co-activator-1 α (PGC-1α)-mediated skeletal muscle regeneration. (A–B) Western blot results of MyoD, Myf5, MEF2 and MyoG in GAS muscle. (C–D) Co-IP results, IP: MyoD, GAS muscle was used. A-B, ∗ p ​< ​0.05 vs. C; ∗∗ p ​< ​0.01 vs. C; ## p ​< ​0.01 vs. Im; $ p ​< ​0.05 vs. E ​+ ​Im; $$ p ​< ​0.01 vs. E ​+ ​Im. Two-way ANOVA was used, and data are shown as means ​± ​standard error of the mean ( SEM ) (C, n ​= ​8, Im, n ​= ​8, E ​+ ​Im, n ​= ​8, E+5003+Im, n ​= ​8). (E–F) H&E staining of myotubes at each differentiation time points of C2C12 myoblasts overexpressing Sesn1. Scale bar ​= ​100 ​μm ∗ p ​< ​0.05 vs. Ad-Sesn1-, unpaired Student's t-test was used and data are shown as means ​± ​ SEM ( n ​= ​3 in each group). (G–H) Western blot results of Sesn1, Prmt1, PGC-1α, MyoD, MEF2, Myf5, and MyoG in C2C12 myoblasts at each time points of differentiation. ∗ p ​< ​0.05 vs. D0, unpaired Student's t -test was used and data are shown as means ​± ​ SEM ( n ​= ​6 in each group). (I–J) Western blot results of PGC-1α in nucleus of C2C12 myoblasts overexpressing Sesn1 at each time points of differentiation. ∗∗ p ​< ​0.01 vs. Ad-Sesn1, unpaired Student's t -test was used and data are shown as means ​± ​ SEM ( n ​= ​6 in each group). (K–L) Western blot results of PGC-1α in cytoplasm of C2C12 myoblasts overexpressing Sesn1 at each time points of differentiation. ∗∗ p ​< ​0.01 vs. Ad-Sesn1-, unpaired Student's t -test was used and data are shown as means ​± ​ SEM ( n ​= ​6 in each group).

Article Snippet: The antibodies are listed below: aDMA (Anti-Asymmetric Di-Methyl Arginine Motif) (1:1 500, Rabbit, Cell Signal Tech, USA), Akt (protein kinase B) (1:2 000, Mouse, Proteintech, USA), pAkt-Ser473 (1:2 000, Rabbit, Cell Signal Tech, USA), AMPKα2 (1:2 000, Rabbit, Cell Signal Tech, USA), pAMPK-Thr172 (1:2 000, Rabbit, Cell Signal Tech, USA), ATF2 (1:2 000, Rabbit, Proteintech, USA), Atrogin-1 (FBXO32) (1:15 000, Mouse, Proteintech, USA), FoxO3a (Forkhead box O3) (1:1 000, Mouse, Proteintech, USA), pFoxO3a-Ser315 (1:2 000, Rabbit, Proteintech, USA), GAPDH (1:5 000, Rabbit, Utibody, CN), IGF-1 (insulin-like growth factor 1) (1:2 000, Mouse, Proteintech, USA), LaminB (1:2 000, Rabbit, Abcam, USA), MEF2 (myocyte enhancer factor 2) (1:2 000, Rabbit, Proteintech, USA), Myf5 (myogenic factor 5) (1:2 000, Rabbit, Abclonal, CN), MyoD (myogenic differentiation antigen) (1:2 000, Rabbit, Proteintech, USA), MyoG (myogenin) (1:2 000, Rabbit, Proteintech, USA). mTOR (mammalian target of rapamycin) (1:2 000, Rabbit, Cell Signal Tech, USA), p38 (1:2 000, Rabbit, WANLEIBIO, CN), p-p38-Thr180/Tyr182 (1:2 000, Rabbit, WANLEIBIO, CN), PGC-1α (1:1 000, Rabbit, Abcam, USA), Prmt1 (1:2 000, Rabbit, Cell Signal Tech, USA), Raptor (1:1 000, Rabbit, Cell Signal Tech, USA), Sesn1 (1:1 000, Rabbit, HUABIO, CN), Sesn1 (1:1 000, Rabbit, Abcam, USA), TRIM63 (MuRF1) (1:2 000, Rabbit, Proteintech, USA).

Techniques: Western Blot, Co-Immunoprecipitation Assay, Staining