Journal:

Article Title: The fasciated ear2 gene encodes a leucine-rich repeat receptor-like protein that regulates shoot meristem proliferation in maize

doi: 10.1101/gad.208501

Figure Lengend Snippet: Molecular characterization of fea2. (A) Southern blot of SstI-digested genomic DNA from (1) fea2-0 homozygous mutant, (2) normal sib, (3) fea2-0 heterozygote, and (4) fea2-0/fea2-846 heterozygote. Note the novel 3 kb polymorphism in this plant associated with the fea2-846 mutation. The probe was the 550-bp fragment downstream of the Mu8 element in fea2-0. Values on the left side represent size in kb. (B) Schematic of predicted domains in FEA2 and CLV2 proteins. (C) CLUSTALW (http://dot.imgen.bcm.tmc.edu:9331/multialign/Help/clustalw.html) alignment of FEA2 (from B73 inbred line; top line) and CLV2 (below, Genbank accession no. AAF02655). Identical residues are outlined in black, similar in gray; dashes represent gaps introduced to optimize the alignment, and “empty” gaps are introduced to separate each LRR motif, according to Thomas et al. (1997). Arrows indicate positions of the Mu transposon insertions in the two fea2 mutant alleles. Predictions of transmembrane and signal sequences are by SMART (http://smart.embl-heidelberg.de/) for FEA2 and from Jeong et al. (1999) for CLV2. These features are labeled above and below the respective sequences. (>>>) Signal peptide; (∼∼∼) transmembrane domain; (* *) cysteine pair.

Article Snippet: Values on the left side represent size in kb. ( B ) Schematic of predicted domains in FEA2 and CLV2 proteins. ( C ) CLUSTALW ( http://dot.imgen.bcm.tmc.edu:9331/multialign/Help/clustalw.html ) alignment of FEA2 (from B73 inbred line; top line) and CLV2 (below, Genbank accession no. {"type":"entrez-protein","attrs":{"text":"AAF02655","term_id":"6049567","term_text":"AAF02655"}} AAF02655 ).

Techniques: Southern Blot, Mutagenesis, Labeling