Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: Expression of neural and neurotrophic markers in nucleus pulposus cells isolated from degenerated intervertebral disc.

doi: 10.1002/jor.22098

Figure Lengend Snippet: Figure 2. Mesenchymal Profile of Human NPs-IVD: (A) Immunophenotypic profile, by citofluorimetric analysis, of nucleus pulposus from 14 human degenerated intervertebral discs. (B) NPs-IVD show mesenchymal properties under chondrogenic, adipogenic, and osteogenic differentiation.

Article Snippet: Human Mesenchymal Stem Cell Functional Identification Kit (R&D Systems, Minneapolis, MN) was used to induce adipogenic, chondrogenic, and osteogenic differentiation.

Techniques:

Journal: Molecular Therapy. Nucleic Acids

Article Title: Ratio of miRNA-29 to miRNA-199 expression coordinates mesenchymal stem cell repair of bleomycin-induced pulmonary injury

doi: 10.1016/j.omtn.2025.102461

Figure Lengend Snippet: Bleomycin-induced lung injury, which is reduced after adipose-derived mesenchymal stem cell instillation (A) Representative μCT transverse and coronal lung sections acquired from aged (22-month-old) male C57BL/6 mice at baseline (left) and 7 days following intratracheal bleomycin (BLM, 2.0 U/kg) administration (right) demonstrating increased lung density and loss of airspaces. (B) Saline treatment did not result in evidence of lung injury on μCT scan at baseline (left) or 7 days post-instillation (right). Histological sections of lung tissue collected at day 21 post-BLM were stained with Masson’s trichrome as described in . (C and D) Representative photomicrographs (20× and 40× magnifications) of lung sections from saline-treated control mice (C) and BLM-treated mice (D). (E) Infusion of adipose-derived mesenchymal stem cells (ASCs) 12 days post-BLM instillation resulted in reduced severity of pulmonary fibrosis (PF). (F) Degree of PF on histological sections was measured by semi-quantitative Ashcroft score as described in . BLM-induced lung injury resulted in increased Ashcroft score compared to saline controls. Infusion with ASCs 12 days post-BLM injury resulted in decreased Ashcroft score. (G) Intratracheal BLM instillation increased lung collagen content as measured by hydroxyproline assays as described in . Mice treated with ASCs on day 12 post-BLM had decreased lung collagen content compared to BLM-only controls. Each data point represents an individual biological replicate (mouse); n = 6–10 mice/group. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. (H) Ratio of pAKT to AKT protein expression in lung tissue of mice was quantified by western blot analysis at day 21 post-BLM sacrifice. Aged C57BL/6 mice treated with intratracheal BLM demonstrated increased pAKT/AKT protein expression compared to saline-treated controls. Lungs from mice treated with intravenous infusion of ASCs 12 days post-BLM-induced injury demonstrated decreased expression of pAKT/AKT compared to BLM-only group. Inset shows a representative western blot and β-actin loading control. Data are graphed as individual biological replicates ( n = 6–8 mice/group); ∗ p < 0.05.

Article Snippet: For mesenchymal differentiation potential, the Mouse Mesenchymal Stem Cell Functional Identification Kit (R&D Systems, Minneapolis, MN) was used according to the manufacturer’s instructions.

Techniques: Derivative Assay, Saline, Staining, Control, Expressing, Western Blot

Journal: Stem cell research

Article Title: Generation of two familial hypercholesterolemia patient-specific induced pluripotent stem cell lines harboring heterozygous mutations in the LDLR gene

doi: 10.1016/j.scr.2024.103463

Figure Lengend Snippet:

Article Snippet: Ectoderm was inducted with the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems #SC027B).

Techniques: Virus, Modification

Journal: bioRxiv

Article Title: Hippocampal CA2 Inhibition Disrupts Prefrontal and Thalamic Connectivity

doi: 10.64898/2026.08.25.746953

Figure Lengend Snippet: a Experimental setup of functional Ultrasound measurements. b Time course of changes in Cerebral Blood Volume (CBV) level in dorsal CA2 during baseline recording and after acute CNO application. Dashed line indicates CNO application. Dark reddish area indicates the investigation period for regional activity (n = 9 mice per group; Ancova, Group mean ± SEM, asterix is p < 0.05, circled asterix is p < 0.01, dark asterix is p < 0.001). c Percentual mean difference in relative CBV (rCBV) of subregions of the dorsal hippocampus (upper), ventral hippocampus (middle) and prefrontal cortex (lower) at minute 40-50 of scan (indicated by reddish area in a ) (raw data are shown in ; 2-tailed unpaired t-test: for dCA1, t(16) = 2.476, p = 0.0248; for dCA2, t(16) = 3.286, p = 0.0047; for vCA3, t(16) = 2.755, p = 0.0141). d Pearson r correlation in relative CBV between dCA2 and mPFC (for Vh, n = 9 mice; for hM4Di + , n = 10 mice). Trend line is generated via simple linear regression. e Global functional connectivity of the mPFC shown as average correlation at baseline and acute CNO (minutes 15-50) (acute for Vh (n = 10 mice) and hM4Di + (n = 10 mice), 2-tailed unpaired t-test: t(18) = 2.285, p = 0.035). f Heatmap of functional connectivity between regions of the hippocampus and frontal cortex in hM4Di + mice relative to controls during acute CNO (minutes 55-90). Dashed square indicates the mPFC-cluster of hypoconnectivity. Statistical difference is shown via Z-scores. Z < 0 is control > hM4Di + (blue color), Z > 0 is Control < hM4Di + (red color). *black: p < 0.05, *grey: p < 0.01, *white: p < 0.001. Otherwise, ns = not significant, *p < 0.05, ** p < 0.01. Statistical details are shown for significant changes only, all details are provided in statistical details file.

Article Snippet: Cerebral blood volume (CBV) of mCherry and hM4Di-mCherry injected Amigo2-Cre + male (n = 11; for Vh n = 5 mice; for hM4Di n = 6 mice) and female (n = 13; for Vh n = 7 mice; for hM4Di n = 6 mice) mice were measured by functional ultrasound imaging (ICONEUS One, ICONEUS, France).

Techniques: Functional Assay, Activity Assay, Generated, Control

Journal: Science Advances

Article Title: The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration

doi: 10.1126/sciadv.abl5942

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: APC anti-human CD11a , Mouse IgG1 , Miltenyi Biotec 130-127-294 , 1:50.

Techniques: Cytometry