Journal: Bone Research

Article Title: C/EBPβ dictates postmenopausal FSHβ transcription and blockade of AEP/C/EBPβ pathway alleviates osteoporosis

doi: 10.1038/s41413-026-00510-y

Figure Lengend Snippet: #11a but not CF3CN suppresses elevation of FSH expression induced by OVX. a Ovariectomized (OVX) mice fed with vehicle, #11a or CF3CN all displayed hypoplastic thread-like uteri and atrophic ovaries, which were normal in sham mice. Uterine weight was quantified and is presented on the right. b The serum FSH levels of sham and OVX mice fed with vehicle, #11a or CF3CN (left); the serum FSH levels of ovariectomized WT and BDNF -/+ mice treated with vehicle or TrkB agonist R13 (right). n = 4–6 mice per group. c Immunoblotting of the pituitary glands from sham, OVX+ vehicle, OVX + #11a and OVX + CF3CN mice showed that #11a but not CF3CN inhibited FSHβ, pC/EBPβ, C/EBPβ and AEP levels induced by OVX. Data are representatives of three independent experiments. d Quantification of western blotting in ( c ), n = 3 per group. e The FSH levels in pituitary gland from sham and OVX mice treated with vehicle, #11a or CF3CN, detected by ELISA. n = 6 mice per group. f The RT-qPCR results of FSHβ and C/EBPβ mRNA levels in the pituitary glands from sham, OVX+ vehicle, OVX + #11a and OVX + CF3CN mice, n = 3 per group. GAPDH was employed as the internal control. Data represented as mean ± SEM, one-way ANOVA, ns no significance, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Serum mouse follicle-stimulating hormone (FSH) ELISA Kit (catalog#: CSB-E06871m-96T) and mouse luteinizing hormone (LH) ELISA kit (catalog#: CSB-E12770m-96T) were from CUSABIO.

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Control

Journal: Bone Research

Article Title: C/EBPβ dictates postmenopausal FSHβ transcription and blockade of AEP/C/EBPβ pathway alleviates osteoporosis

doi: 10.1038/s41413-026-00510-y

Figure Lengend Snippet: CF3CN and #11a inhibit osteoclastogenesis. a Representative images (left) and quantification (right) of Tartrate-resistant acid phosphatase-stained (TRAP-stained) sections of the distal femur bone. Scale bar = 500 μm (upper panel), Scale bar = 100 μm (lower panel). Areas of interest used in quantification were randomly selected around the circle-marked regions. Area 1 is located near the distal growth plate, area 2 represents cortical or trabecular bone. n = 3 slice from three mice. b The in-vitro bone resorption assay of RAW 264.7 cells induced by RANK-L with or without treatment of BDNF, 7,8-DHF, CF3CN or #11a for 10 days. Scale bar = 100 μm for toluidine blue staining images (upper). Scale bar = 50 μm for TRAP staining images (lower). c ELISA assays detecting CTX1, a bone resorption marker. Serum levels of CTX1 were measured in sham-operated or OVX mice treated with vehicle, CF3CN, or #11a. CTX1 levels were also assessed in the culture medium of undifferentiated RAW 264.7 cells, as well as in differentiated RAW 264.7 cells treated with vehicle, BDNF, 7,8-DHF, CF3CN, or #11a. d , e Images and quantification of Western blotting of RAW 264.7 cells induced by RANK-L with or without treatment of BDNF, 7,8-DHF, CF3CN or #11a for 4 days. ( n = 3, one-way ANOVA). f AEP enzymatic activity assay of RAW 264.7 cells induced by RANK-L with or without treatment of BDNF, 7,8-DHF, CF3CN or #11a for 4 days. ( n = 4, one-way ANOVA). g Representative images of immunohistochemistry staining of C/EBPβ and AEP of the distal femur bone. Data represented as mean ± SEM, ns no significance, one-way ANOVA, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Serum mouse follicle-stimulating hormone (FSH) ELISA Kit (catalog#: CSB-E06871m-96T) and mouse luteinizing hormone (LH) ELISA kit (catalog#: CSB-E12770m-96T) were from CUSABIO.

Techniques: Staining, In Vitro, Enzyme-linked Immunosorbent Assay, Marker, Western Blot, Enzyme Activity Assay, Immunohistochemistry