Journal: Nucleic Acids Research

Article Title: RNase W, a conserved ribonuclease family with a novel active site

doi: 10.1093/nar/gkae907

Figure Lengend Snippet: X-ray diffraction and refinement statistics

Article Snippet: The open reading frames of RNase W (FAU-1) from P. furiosus (full-length protein, constructs and mutants; PF0022) and S. acidocadarius (Saci_1213) were synthesized commercially by GenScript Corp. (Piscatawy, NJ) and inserted in pET24(a+) (Novagen), pET24- Pf RNase W and pET24- Sa RNase W, respectively.

Techniques: X-ray Diffraction

Journal: Nucleic Acids Research

Article Title: RNase W, a conserved ribonuclease family with a novel active site

doi: 10.1093/nar/gkae907

Figure Lengend Snippet: Structures of RNase W from P. furiosus and S. acidocaldarius . ( A and B ) Structures of RNase W proteins colored by domains: N-terminal domain in orange, S1 domain in dark green, 5′-sensor domain in light green, α helical bundle domain in red and DUF402 domain in blue. ( A ) Structure of RNase W from P. furiosus ( Pf RNase W). A dinucleotide UU with 5′ and 3′-phosphate extremities is bound to the 5′-sensor domain. In addition, a dinucleotide UA with 5′-hydroxyl and 3′-phosphate extremities is bound to the DUF402 domain. ( B ) Structure of RNase W from S. acidocaldarius ( Sa RNase W). ( C ) Pf RNase W surface representation with residues conservation score in archaeal RNase W proteins mapped on the surface. The encircled central domain includes the N-terminal domain and one helix of the α helical bundle domain annotated in panel A. ( D ) Electrostatic surface potential mapped on the surface of Pf RNase W protein.

Article Snippet: The open reading frames of RNase W (FAU-1) from P. furiosus (full-length protein, constructs and mutants; PF0022) and S. acidocadarius (Saci_1213) were synthesized commercially by GenScript Corp. (Piscatawy, NJ) and inserted in pET24(a+) (Novagen), pET24- Pf RNase W and pET24- Sa RNase W, respectively.

Techniques:

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: In vivo visualization of photoreceptor layer and lipofuscin accumulation in stargardt’s disease and fundus flavimaculatus by high resolution spectral-domain optical coherence tomography

doi:

Figure Lengend Snippet: Criteria for inclusion/exclusion

Article Snippet: All patients underwent a complete ophthalmologic examination, including assessment of best-corrected visual acuity (BCVA) measured at 4 m with standard Early Treatment Diabetic Retinopathy Study (ETDRS), fundus biomicroscopy, color photography and red free frame of the fundus (Canon 60 fundus camera, Tokyo, Japan; Topcon TRC-50 retinal camera, Tokyo, Japan), autofluorescence frames (Heidelberg Retina Angiograph II, Heidelberg Engineering, Heidelberg, Germany), red free and FA frames (Canon 60 fundus camera; Topcon TRC-50 retinal camera).

Techniques: Membrane

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: In vivo visualization of photoreceptor layer and lipofuscin accumulation in stargardt’s disease and fundus flavimaculatus by high resolution spectral-domain optical coherence tomography

doi:

Figure Lengend Snippet: Case 4 fundus autofluorescence and high definition spectral domain optical coherence tomography (HD-OCT). Fundus autofluorescent frame of the left eye (20/25 best corrected visual acuity) shows mottled autofluorescence in the macular area and retinal flecks (thin arrows). HD-OCT scans ( A and B ) show disruption of either the layer between the retinal pigment epithelium (RPE) and the outer segment (OS) of the photoreceptors (PR) (enlarged view), either the layer corresponding to the interface of inner segment (IS) and OS of PR in the foveal region (enlarged view; asterisks), and a focal loss of the PR layer in the parafoveal region (open arrows).

Article Snippet: All patients underwent a complete ophthalmologic examination, including assessment of best-corrected visual acuity (BCVA) measured at 4 m with standard Early Treatment Diabetic Retinopathy Study (ETDRS), fundus biomicroscopy, color photography and red free frame of the fundus (Canon 60 fundus camera, Tokyo, Japan; Topcon TRC-50 retinal camera, Tokyo, Japan), autofluorescence frames (Heidelberg Retina Angiograph II, Heidelberg Engineering, Heidelberg, Germany), red free and FA frames (Canon 60 fundus camera; Topcon TRC-50 retinal camera).

Techniques: Tomography, Disruption

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: In vivo visualization of photoreceptor layer and lipofuscin accumulation in stargardt’s disease and fundus flavimaculatus by high resolution spectral-domain optical coherence tomography

doi:

Figure Lengend Snippet: Case 8 fundus autofluorescence and high definition spectral domain optical coherence tomography (HD-OCT). Fundus autofluorescent frame of the left eye (20/125 best corrected visual acuity) shows mottled autofluorescence in the macular area and clearly delineates the retinal flecks. HD-OCT scans ( A , B , and C ) show a diffuse loss of the photoreceptor layer (enlarged view) in the foveal region ( B , open arrows), and small hyperreflective lesions presented as dome-shaped deposits located within the retinal pigment epithelium ( A and C , thin arrows).

Article Snippet: All patients underwent a complete ophthalmologic examination, including assessment of best-corrected visual acuity (BCVA) measured at 4 m with standard Early Treatment Diabetic Retinopathy Study (ETDRS), fundus biomicroscopy, color photography and red free frame of the fundus (Canon 60 fundus camera, Tokyo, Japan; Topcon TRC-50 retinal camera, Tokyo, Japan), autofluorescence frames (Heidelberg Retina Angiograph II, Heidelberg Engineering, Heidelberg, Germany), red free and FA frames (Canon 60 fundus camera; Topcon TRC-50 retinal camera).

Techniques: Tomography

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: In vivo visualization of photoreceptor layer and lipofuscin accumulation in stargardt’s disease and fundus flavimaculatus by high resolution spectral-domain optical coherence tomography

doi:

Figure Lengend Snippet: Case 2 fundus autofluorescence and high definition spectral domain optical coherence tomography (HD-OCT). Fundus autofluorescent frame of the right eye (20/20 best corrected visual acuity) shows mottled autofluorescence in the macular area and clearly delineates the retinal flecks. HD-OCT scans ( A, B, and C ) show an intact inner segment and outer segment interface of the photoreceptors (PR) centrally (enlarged view), but diffuse parafoveal loss of the PR layer (open arrows), and small hyperreflective lesions presented either as dome-shaped deposits within the retinal pigment epithelium (RPE) ( B , thin arrow), or as small linear deposits located at the level of the outer nuclear layer and clearly separated from the RPE ( A and C , arrowheads).

Article Snippet: All patients underwent a complete ophthalmologic examination, including assessment of best-corrected visual acuity (BCVA) measured at 4 m with standard Early Treatment Diabetic Retinopathy Study (ETDRS), fundus biomicroscopy, color photography and red free frame of the fundus (Canon 60 fundus camera, Tokyo, Japan; Topcon TRC-50 retinal camera, Tokyo, Japan), autofluorescence frames (Heidelberg Retina Angiograph II, Heidelberg Engineering, Heidelberg, Germany), red free and FA frames (Canon 60 fundus camera; Topcon TRC-50 retinal camera).

Techniques: Tomography

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: In vivo visualization of photoreceptor layer and lipofuscin accumulation in stargardt’s disease and fundus flavimaculatus by high resolution spectral-domain optical coherence tomography

doi:

Figure Lengend Snippet: A ) Case 5 fundus autofluorescence and high definition spectral domain optical coherence tomography (HD-OCT). Fundus autofluorescent frame of the right eye (20/25 best corrected visual acuity) shows mottled autofluorescence in the macular area and several diffuse retinal flecks. HD-OCT scans show disruption of either the layer between the retinal pigment epithelium (RPE) and the outer segment (OS) of the photoreceptors (PR), either the layer corresponding to the interface of inner segment (IS) and OS of PR in the foveal region (asterisk), and a focal loss of the PR layer in the parafoveal region (open arrows). Small, large and very large hyperreflective lesions presented as dome-shaped deposits located within the RPE and at the level of the outer segments of PR (thin arrows), and small linear deposits located at the level of the outer nuclear layer and clearly separated from the RPE layer (arrowhead), are visualized by HD-OCT scans. B ) Case 1 fluorescein angiography and HD-OCT. Fluorescein angiography frame of the left eye (20/50 best corrected visual acuity) shows mottled fluorescence in the macular area and several diffuse retinal flecks. HD-OCT scans show a focal loss of the photoreceptor layer in the foveal region (open arrows), and very large hyperreflective lesions presented as dome-shaped deposits located within the retinal pigment epithelium (thin arrows).

Article Snippet: All patients underwent a complete ophthalmologic examination, including assessment of best-corrected visual acuity (BCVA) measured at 4 m with standard Early Treatment Diabetic Retinopathy Study (ETDRS), fundus biomicroscopy, color photography and red free frame of the fundus (Canon 60 fundus camera, Tokyo, Japan; Topcon TRC-50 retinal camera, Tokyo, Japan), autofluorescence frames (Heidelberg Retina Angiograph II, Heidelberg Engineering, Heidelberg, Germany), red free and FA frames (Canon 60 fundus camera; Topcon TRC-50 retinal camera).

Techniques: Tomography, Disruption, Fluorescence