formamide 2xssc Search Results


hybridisation buffer  (Thermo Fisher)
99
Thermo Fisher hybridisation buffer
Hybridisation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+2xssc/Tween+20/pmc08758788-398-40-35
Average 99 stars, based on 1 article reviews
hybridisation buffer - by Bioz Stars, 2026-10
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hybridization buffer  (Thermo Fisher)
99
Thermo Fisher hybridization buffer
a ) Principle of mutation-based cell labeling with STAR-FACS for TERT promoter hotspot mutation C228T. First, the genomic DNA region of interest (597bp fragment of TERT promoter) in intact fixed cells is subject to PCR amplification. In the second round PCR, mutation-specific primers with unique overhangs are used. The primers contain mismatches, enabling efficient amplification of the mutant, but not the wild-type amplicon. The unique overhangs incorporated into the PCR product in the 2nd round PCR allows for specific <t>hybridization</t> of fluorescently labeled probes. Labeled intact cells can be then FACS-sorted. b ) Fixed and permeabilized cells can be cryopreserved and labeled by two-step in cell PCR followed by fluorescent probe hybridization. This enables the sorting of cells for downstream applications, including RNA-seq, scRNA-seq and CUT&Tag.
Hybridization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+2xssc/DEXTRAN/bio_rxiv__2024__07__05__602247-217-19-35
Average 99 stars, based on 1 article reviews
hybridization buffer - by Bioz Stars, 2026-10
99/100 stars
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washing buffer b  (Thermo Fisher)
99
Thermo Fisher washing buffer b
a ) Principle of mutation-based cell labeling with STAR-FACS for TERT promoter hotspot mutation C228T. First, the genomic DNA region of interest (597bp fragment of TERT promoter) in intact fixed cells is subject to PCR amplification. In the second round PCR, mutation-specific primers with unique overhangs are used. The primers contain mismatches, enabling efficient amplification of the mutant, but not the wild-type amplicon. The unique overhangs incorporated into the PCR product in the 2nd round PCR allows for specific <t>hybridization</t> of fluorescently labeled probes. Labeled intact cells can be then FACS-sorted. b ) Fixed and permeabilized cells can be cryopreserved and labeled by two-step in cell PCR followed by fluorescent probe hybridization. This enables the sorting of cells for downstream applications, including RNA-seq, scRNA-seq and CUT&Tag.
Washing Buffer B, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+2xssc/SSC+buffer/pmc11044841-497-23-31
Average 99 stars, based on 1 article reviews
washing buffer b - by Bioz Stars, 2026-10
99/100 stars
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dna staining  (Thermo Fisher)
99
Thermo Fisher dna staining
a ) Principle of mutation-based cell labeling with STAR-FACS for TERT promoter hotspot mutation C228T. First, the genomic DNA region of interest (597bp fragment of TERT promoter) in intact fixed cells is subject to PCR amplification. In the second round PCR, mutation-specific primers with unique overhangs are used. The primers contain mismatches, enabling efficient amplification of the mutant, but not the wild-type amplicon. The unique overhangs incorporated into the PCR product in the 2nd round PCR allows for specific <t>hybridization</t> of fluorescently labeled probes. Labeled intact cells can be then FACS-sorted. b ) Fixed and permeabilized cells can be cryopreserved and labeled by two-step in cell PCR followed by fluorescent probe hybridization. This enables the sorting of cells for downstream applications, including RNA-seq, scRNA-seq and CUT&Tag.
Dna Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+2xssc/DNA/pmc11043350-331-23-33
Average 99 stars, based on 1 article reviews
dna staining - by Bioz Stars, 2026-10
99/100 stars
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hybridization probe  (LGC Biosearch)
90
LGC Biosearch hybridization probe
a ) Principle of mutation-based cell labeling with STAR-FACS for TERT promoter hotspot mutation C228T. First, the genomic DNA region of interest (597bp fragment of TERT promoter) in intact fixed cells is subject to PCR amplification. In the second round PCR, mutation-specific primers with unique overhangs are used. The primers contain mismatches, enabling efficient amplification of the mutant, but not the wild-type amplicon. The unique overhangs incorporated into the PCR product in the 2nd round PCR allows for specific <t>hybridization</t> of fluorescently labeled probes. Labeled intact cells can be then FACS-sorted. b ) Fixed and permeabilized cells can be cryopreserved and labeled by two-step in cell PCR followed by fluorescent probe hybridization. This enables the sorting of cells for downstream applications, including RNA-seq, scRNA-seq and CUT&Tag.
Hybridization Probe, supplied by LGC Biosearch, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+2xssc/hybridization+probe/bio_rxiv__740498-68-2-16
Average 90 stars, based on 1 article reviews
hybridization probe - by Bioz Stars, 2026-10
90/100 stars
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fish wash buffer  (Biosearch Technologies Inc)
86
Biosearch Technologies Inc fish wash buffer
a ) Principle of mutation-based cell labeling with STAR-FACS for TERT promoter hotspot mutation C228T. First, the genomic DNA region of interest (597bp fragment of TERT promoter) in intact fixed cells is subject to PCR amplification. In the second round PCR, mutation-specific primers with unique overhangs are used. The primers contain mismatches, enabling efficient amplification of the mutant, but not the wild-type amplicon. The unique overhangs incorporated into the PCR product in the 2nd round PCR allows for specific <t>hybridization</t> of fluorescently labeled probes. Labeled intact cells can be then FACS-sorted. b ) Fixed and permeabilized cells can be cryopreserved and labeled by two-step in cell PCR followed by fluorescent probe hybridization. This enables the sorting of cells for downstream applications, including RNA-seq, scRNA-seq and CUT&Tag.
Fish Wash Buffer, supplied by Biosearch Technologies Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+2xssc/a+buffer+wash/pm39652010-236-9-31
Average 86 stars, based on 1 article reviews
fish wash buffer - by Bioz Stars, 2026-10
86/100 stars
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formamide 2x ssc  (Thermo Fisher)
99
Thermo Fisher formamide 2x ssc
a ) Principle of mutation-based cell labeling with STAR-FACS for TERT promoter hotspot mutation C228T. First, the genomic DNA region of interest (597bp fragment of TERT promoter) in intact fixed cells is subject to PCR amplification. In the second round PCR, mutation-specific primers with unique overhangs are used. The primers contain mismatches, enabling efficient amplification of the mutant, but not the wild-type amplicon. The unique overhangs incorporated into the PCR product in the 2nd round PCR allows for specific <t>hybridization</t> of fluorescently labeled probes. Labeled intact cells can be then FACS-sorted. b ) Fixed and permeabilized cells can be cryopreserved and labeled by two-step in cell PCR followed by fluorescent probe hybridization. This enables the sorting of cells for downstream applications, including RNA-seq, scRNA-seq and CUT&Tag.
Formamide 2x Ssc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+2xssc/SSC/pm19224609-48-16-38
Average 99 stars, based on 1 article reviews
formamide 2x ssc - by Bioz Stars, 2026-10
99/100 stars
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hybridization buffer  (New England Biolabs)
98
New England Biolabs hybridization buffer
a ) Principle of mutation-based cell labeling with STAR-FACS for TERT promoter hotspot mutation C228T. First, the genomic DNA region of interest (597bp fragment of TERT promoter) in intact fixed cells is subject to PCR amplification. In the second round PCR, mutation-specific primers with unique overhangs are used. The primers contain mismatches, enabling efficient amplification of the mutant, but not the wild-type amplicon. The unique overhangs incorporated into the PCR product in the 2nd round PCR allows for specific <t>hybridization</t> of fluorescently labeled probes. Labeled intact cells can be then FACS-sorted. b ) Fixed and permeabilized cells can be cryopreserved and labeled by two-step in cell PCR followed by fluorescent probe hybridization. This enables the sorting of cells for downstream applications, including RNA-seq, scRNA-seq and CUT&Tag.
Hybridization Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+2xssc/Ribonucleoside-Vanadyl+Complex/pmc05590993-68-24-52
Average 98 stars, based on 1 article reviews
hybridization buffer - by Bioz Stars, 2026-10
98/100 stars
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wash buffer  (Thermo Fisher)
97
Thermo Fisher wash buffer
a ) Principle of mutation-based cell labeling with STAR-FACS for TERT promoter hotspot mutation C228T. First, the genomic DNA region of interest (597bp fragment of TERT promoter) in intact fixed cells is subject to PCR amplification. In the second round PCR, mutation-specific primers with unique overhangs are used. The primers contain mismatches, enabling efficient amplification of the mutant, but not the wild-type amplicon. The unique overhangs incorporated into the PCR product in the 2nd round PCR allows for specific <t>hybridization</t> of fluorescently labeled probes. Labeled intact cells can be then FACS-sorted. b ) Fixed and permeabilized cells can be cryopreserved and labeled by two-step in cell PCR followed by fluorescent probe hybridization. This enables the sorting of cells for downstream applications, including RNA-seq, scRNA-seq and CUT&Tag.
Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+2xssc/SSC+buffer/cullinane_danielle_l__2014__mechanisms_of_translational_repression_of_the_sperm_mitochondria_associated_cysteine_rich_protein-1411-16-23
Average 97 stars, based on 1 article reviews
wash buffer - by Bioz Stars, 2026-10
97/100 stars
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probe mixture  (Eurofins)
86
Eurofins probe mixture
a ) Principle of mutation-based cell labeling with STAR-FACS for TERT promoter hotspot mutation C228T. First, the genomic DNA region of interest (597bp fragment of TERT promoter) in intact fixed cells is subject to PCR amplification. In the second round PCR, mutation-specific primers with unique overhangs are used. The primers contain mismatches, enabling efficient amplification of the mutant, but not the wild-type amplicon. The unique overhangs incorporated into the PCR product in the 2nd round PCR allows for specific <t>hybridization</t> of fluorescently labeled probes. Labeled intact cells can be then FACS-sorted. b ) Fixed and permeabilized cells can be cryopreserved and labeled by two-step in cell PCR followed by fluorescent probe hybridization. This enables the sorting of cells for downstream applications, including RNA-seq, scRNA-seq and CUT&Tag.
Probe Mixture, supplied by Eurofins, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formamide+2xssc/mixture+probe/bio_rxiv__2025__10__23__681605-404-22-38
Average 86 stars, based on 1 article reviews
probe mixture - by Bioz Stars, 2026-10
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Image Search Results


a ) Principle of mutation-based cell labeling with STAR-FACS for TERT promoter hotspot mutation C228T. First, the genomic DNA region of interest (597bp fragment of TERT promoter) in intact fixed cells is subject to PCR amplification. In the second round PCR, mutation-specific primers with unique overhangs are used. The primers contain mismatches, enabling efficient amplification of the mutant, but not the wild-type amplicon. The unique overhangs incorporated into the PCR product in the 2nd round PCR allows for specific hybridization of fluorescently labeled probes. Labeled intact cells can be then FACS-sorted. b ) Fixed and permeabilized cells can be cryopreserved and labeled by two-step in cell PCR followed by fluorescent probe hybridization. This enables the sorting of cells for downstream applications, including RNA-seq, scRNA-seq and CUT&Tag.

Journal: bioRxiv

Article Title: Cell sorting based on single nucleotide variation enables characterization of mutation-dependent transcriptome and chromatin states

doi: 10.1101/2024.07.05.602247

Figure Lengend Snippet: a ) Principle of mutation-based cell labeling with STAR-FACS for TERT promoter hotspot mutation C228T. First, the genomic DNA region of interest (597bp fragment of TERT promoter) in intact fixed cells is subject to PCR amplification. In the second round PCR, mutation-specific primers with unique overhangs are used. The primers contain mismatches, enabling efficient amplification of the mutant, but not the wild-type amplicon. The unique overhangs incorporated into the PCR product in the 2nd round PCR allows for specific hybridization of fluorescently labeled probes. Labeled intact cells can be then FACS-sorted. b ) Fixed and permeabilized cells can be cryopreserved and labeled by two-step in cell PCR followed by fluorescent probe hybridization. This enables the sorting of cells for downstream applications, including RNA-seq, scRNA-seq and CUT&Tag.

Article Snippet: The hybridization mix (sufficient for a total of ∼1 x 10 6 cells) was prepared with 215.31 μL of hybridization buffer (2x SSC, 20% formamide, 10% dextran sulfate), 25.9 μL of 1mg/mL human COT1 DNA (Life Technologies), 2.59 μL of 10 mg/mL shredded salmon sperm DNA (Life Technologies; both COT1 and salmon sperm DNA were biotinylated with EZ-Link Psoralen-PEG3-Biotin via UV activated intercalation prior to their use in the buffer to facilitate their removal when preparing the labelled cells for sequencing) and 6.2 μL mutation specific fluorescent probe (the for the simultaneous labeling of the C228T and C250T mutation, 4 μL of each probe were used).

Techniques: Mutagenesis, Labeling, Amplification, Hybridization, RNA Sequencing