fluorogenic acetylated hdac substrate 3 Search Results


3.3,5.5-tetramethylbenzidine substrate  (BioFX Laboratories Inc)
90
BioFX Laboratories Inc 3.3,5.5-tetramethylbenzidine substrate
3.3,5.5 Tetramethylbenzidine Substrate, supplied by BioFX Laboratories Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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substrate 3 3 5 5 tetramethylbenzidine tmb  (Thermo Fisher)
99
Thermo Fisher substrate 3 3 5 5 tetramethylbenzidine tmb
Substrate 3 3 5 5 Tetramethylbenzidine Tmb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3-amino-9-ethylcarbazole (aec  (Becton Dickinson)
90
Becton Dickinson 3-amino-9-ethylcarbazole (aec
3 Amino 9 Ethylcarbazole (Aec, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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color substrate 3 3 5 5 tetramethylbenzidine  (Millipore)
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Millipore color substrate 3 3 5 5 tetramethylbenzidine
Color Substrate 3 3 5 5 Tetramethylbenzidine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quartz substrate  (Ted Pella)
86
Ted Pella quartz substrate
Quartz Substrate, supplied by Ted Pella, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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insulin receptor substrate 1 irs 1 phospho tyr608  (Danaher Inc)
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Danaher Inc insulin receptor substrate 1 irs 1 phospho tyr608
Insulin Receptor Substrate 1 Irs 1 Phospho Tyr608, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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colorimetric peroxide enzyme immunoassay substrate  (Bio-Rad)
95
Bio-Rad colorimetric peroxide enzyme immunoassay substrate
Colorimetric Peroxide Enzyme Immunoassay Substrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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insulin receptor substrate 1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology insulin receptor substrate 1
Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) <t>Insulin</t> <t>receptor</t> <t>β</t> (IRβ), insulin receptor substrate 1 <t>(IRS1),</t> phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.
Insulin Receptor Substrate 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3,3 ,5,5 -tetramethylbenzidine peroxidase substrate  (SeraCare Life Sciences)
90
SeraCare Life Sciences 3,3 ,5,5 -tetramethylbenzidine peroxidase substrate
Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) <t>Insulin</t> <t>receptor</t> <t>β</t> (IRβ), insulin receptor substrate 1 <t>(IRS1),</t> phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.
3,3 ,5,5 Tetramethylbenzidine Peroxidase Substrate, supplied by SeraCare Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorogenic+acetylated+hdac+substrate+3/abts+peroxidase+substrate/10__1128_slash_iai__73__11__7485___7494__2005-81-61-68
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3-phenyl-propionitrile  (Vorwerk Elektrowerke GmbH Co KG)
90
Vorwerk Elektrowerke GmbH Co KG 3-phenyl-propionitrile
Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) <t>Insulin</t> <t>receptor</t> <t>β</t> (IRβ), insulin receptor substrate 1 <t>(IRS1),</t> phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.
3 Phenyl Propionitrile, supplied by Vorwerk Elektrowerke GmbH Co KG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eagle2000tm glass substrate  (Corning Life Sciences)
90
Corning Life Sciences eagle2000tm glass substrate
Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) <t>Insulin</t> <t>receptor</t> <t>β</t> (IRβ), insulin receptor substrate 1 <t>(IRS1),</t> phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.
Eagle2000tm Glass Substrate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) Insulin receptor β (IRβ), insulin receptor substrate 1 (IRS1), phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.

Journal: The journals of gerontology. Series A, Biological sciences and medical sciences

Article Title: Differential Development of Inflammation and Insulin Resistance in Different Adipose Tissue Depots Along Aging in Wistar Rats: Effects of Caloric Restriction.

doi: 10.1093/gerona/glv117

Figure Lengend Snippet: Figure 2. Effect of aging and caloric restriction (CR) on insulin-stimulated insulin receptor (IR) and AKT phosphorylation and on insulin signaling pathway in different adipose tissue depots. (A) Ex vivo adipose tissue explants from epididymal white adipose tissue (eWAT), perirenal WAT (pWAT), subcutaneous WAT (sWAT), and brown adipose tissue (BAT) of 3-, 8-, and 24-month-old rats fed ad libitum (AL), and 8- and 24-month-old caloric-restricted rats were stimulated with 80 nM insulin or saline for 10 minutes as described in Methods section. Whole extracts were subjected to Western blot analysis. For insulin-stimulated IR, Tyr phosphorylation membranes were blotted with anti-phospho-Tyr-IRβ (P-Tyr-IRβ) and anti-IRβ antibodies. For insulin-stimulated AKT phosphorylation, membranes were blotted with anti-phospho-AKT (P-AKT) and anti-AKT antibodies. Upper panels show representative Western blots. Bars, in the lower panels, represent quantification of P-Tyr-IRβ and P-AKT after correction for IRβ and AKT, respectively, by scanning densitometry. Results are expressed as fold increase of IR and AKT phosphorylation. (B) Insulin receptor β (IRβ), insulin receptor substrate 1 (IRS1), phospho-Ser IRS1 (P-Ser-IRS1), and PTP1B levels were analyzed in whole adipose extracts by Western blot. Membranes were blotted with anti-IRβ, anti-IRS1, anti-P-Ser-IRS1, and anti-PTP1B antibodies. Anti-β-actin antibodies were used as a loading control. Upper panels show representative Western blots. Bars, in the lower panels, represent quantifications by scanning densitometry after correction for β-actin. Results, expressed as arbitrary densitometric units (A.D.U.) relative to 3-month-old rats equaled to 100, are means ± SEM of 5–8 animals per group. The data AL were evaluated by one-way analysis of variance test followed by Tukey’s post hoc test. *p < .05, **p < .01, ***p < .001 vs 3-month- old rats. CR was compared with its AL age-mate group using nonpaired Student’s t test. #p < .05, ##p < .01 vs same age-fed AL.

Article Snippet: Antibodies directed toward phospho IRβ (Tyr 1162/1163), insulin-Rβ (C-19), insulin receptor substrate 1 (IRS1; C-20), and PTP1B (H135) were from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Phospho-proteomics, Ex Vivo, Saline, Western Blot, Control