fluorescent ubiquitin Search Results


fluorescent ubiquitin lifesensors  (LifeSensors)
90
LifeSensors fluorescent ubiquitin lifesensors
Chemical activation of the C terminus of <t>ubiquitin</t> as a thioester (e.g. UbMES or UbFluor) can bypass the need for E1, E2, and ATP, downsizing the 5-component native cascade reaction (E1, E2, ATP, PARKIN, and Ub) to 2 components (PARKIN and ByS probes). Transthiolation with UbMES releases a mercaptoethanesulfonate group (MES), whereas transthiolation with UbFluor releases fluorescein thiol.
Fluorescent Ubiquitin Lifesensors, supplied by LifeSensors, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ubiquitin/fluorescent+ubiquitin+lifesensors/pmc05633118-567-18-21
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k63 linked internally quenched fluorescence based di-ubiquitin substrate  (LifeSensors)
90
LifeSensors k63 linked internally quenched fluorescence based di-ubiquitin substrate
DUBs examined for behavior against ubiquitylated substrates were active enzymes. A. The fourteen DUBs examined in <xref ref-type=Fig. 2 all showed activity (signal versus time over 1 h) by CHOP reporter assay (LifeSensors, Inc.), except AMSH. B. AMSH was examined for activity (versus time over 1 h) against different substrates, di-ubiquitin substrates labeled with an internally quenched fluorophore pair. As expected, activity was specific to K63-linkage of the diubiquitin. C. E3Lite assay data (endpoint RFU signal) for triplicate wells either ubiquitylated with E3 mix (Praja1, Carp2, Murf1) and then mock treated with DUB (“No DUB”); mock ubiquitylated using reaction mixture lacking an E3 and then mock treated with DUB (“Not UB'd”); or ubiquitylated with E3 mix and then treated with DUB enzyme (as listed). " width="250" height="auto" />
K63 Linked Internally Quenched Fluorescence Based Di Ubiquitin Substrate, supplied by LifeSensors, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ubiquitin/k63+linked+internally+quenched+fluorescence+based+di+ubiquitin+substrate/pmc07113913-122-15-23
Average 90 stars, based on 1 article reviews
k63 linked internally quenched fluorescence based di-ubiquitin substrate - by Bioz Stars, 2026-09
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fluorescent ubiquitination-based cell cycle indicator (fucci) h1 hescs  (GenScript corporation)
90
GenScript corporation fluorescent ubiquitination-based cell cycle indicator (fucci) h1 hescs
DUBs examined for behavior against ubiquitylated substrates were active enzymes. A. The fourteen DUBs examined in <xref ref-type=Fig. 2 all showed activity (signal versus time over 1 h) by CHOP reporter assay (LifeSensors, Inc.), except AMSH. B. AMSH was examined for activity (versus time over 1 h) against different substrates, di-ubiquitin substrates labeled with an internally quenched fluorophore pair. As expected, activity was specific to K63-linkage of the diubiquitin. C. E3Lite assay data (endpoint RFU signal) for triplicate wells either ubiquitylated with E3 mix (Praja1, Carp2, Murf1) and then mock treated with DUB (“No DUB”); mock ubiquitylated using reaction mixture lacking an E3 and then mock treated with DUB (“Not UB'd”); or ubiquitylated with E3 mix and then treated with DUB enzyme (as listed). " width="250" height="auto" />
Fluorescent Ubiquitination Based Cell Cycle Indicator (Fucci) H1 Hescs, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ubiquitin/fluorescent+ubiquitination+based+cell+cycle+indicator++fucci++h1+hescs/pmc04589503-68-0-26
Average 90 stars, based on 1 article reviews
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fluorescent ubiquitination-based cell cycle indicator fucci  (MBL Life science)
90
MBL Life science fluorescent ubiquitination-based cell cycle indicator fucci
DUBs examined for behavior against ubiquitylated substrates were active enzymes. A. The fourteen DUBs examined in <xref ref-type=Fig. 2 all showed activity (signal versus time over 1 h) by CHOP reporter assay (LifeSensors, Inc.), except AMSH. B. AMSH was examined for activity (versus time over 1 h) against different substrates, di-ubiquitin substrates labeled with an internally quenched fluorophore pair. As expected, activity was specific to K63-linkage of the diubiquitin. C. E3Lite assay data (endpoint RFU signal) for triplicate wells either ubiquitylated with E3 mix (Praja1, Carp2, Murf1) and then mock treated with DUB (“No DUB”); mock ubiquitylated using reaction mixture lacking an E3 and then mock treated with DUB (“Not UB'd”); or ubiquitylated with E3 mix and then treated with DUB enzyme (as listed). " width="250" height="auto" />
Fluorescent Ubiquitination Based Cell Cycle Indicator Fucci, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ubiquitin/fluorescent+ubiquitination+based+cell+cycle+indicator++fucci/pm33813383-84-14-22
Average 90 stars, based on 1 article reviews
fluorescent ubiquitination-based cell cycle indicator fucci - by Bioz Stars, 2026-09
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fluorescent ubiquitinated cell cycle indicator (fucci)  (Monsanto Technology LLC)
90
Monsanto Technology LLC fluorescent ubiquitinated cell cycle indicator (fucci)
DUBs examined for behavior against ubiquitylated substrates were active enzymes. A. The fourteen DUBs examined in <xref ref-type=Fig. 2 all showed activity (signal versus time over 1 h) by CHOP reporter assay (LifeSensors, Inc.), except AMSH. B. AMSH was examined for activity (versus time over 1 h) against different substrates, di-ubiquitin substrates labeled with an internally quenched fluorophore pair. As expected, activity was specific to K63-linkage of the diubiquitin. C. E3Lite assay data (endpoint RFU signal) for triplicate wells either ubiquitylated with E3 mix (Praja1, Carp2, Murf1) and then mock treated with DUB (“No DUB”); mock ubiquitylated using reaction mixture lacking an E3 and then mock treated with DUB (“Not UB'd”); or ubiquitylated with E3 mix and then treated with DUB enzyme (as listed). " width="250" height="auto" />
Fluorescent Ubiquitinated Cell Cycle Indicator (Fucci), supplied by Monsanto Technology LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ubiquitin/fluorescent+ubiquitinated+cell+cycle+indicator++fucci+/pm31757861-335-36-19
Average 90 stars, based on 1 article reviews
fluorescent ubiquitinated cell cycle indicator (fucci) - by Bioz Stars, 2026-09
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fluorescence ubiquitination-based cell-cycle indicator (fucci)  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare fluorescence ubiquitination-based cell-cycle indicator (fucci)
DUBs examined for behavior against ubiquitylated substrates were active enzymes. A. The fourteen DUBs examined in <xref ref-type=Fig. 2 all showed activity (signal versus time over 1 h) by CHOP reporter assay (LifeSensors, Inc.), except AMSH. B. AMSH was examined for activity (versus time over 1 h) against different substrates, di-ubiquitin substrates labeled with an internally quenched fluorophore pair. As expected, activity was specific to K63-linkage of the diubiquitin. C. E3Lite assay data (endpoint RFU signal) for triplicate wells either ubiquitylated with E3 mix (Praja1, Carp2, Murf1) and then mock treated with DUB (“No DUB”); mock ubiquitylated using reaction mixture lacking an E3 and then mock treated with DUB (“Not UB'd”); or ubiquitylated with E3 mix and then treated with DUB enzyme (as listed). " width="250" height="auto" />
Fluorescence Ubiquitination Based Cell Cycle Indicator (Fucci), supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ubiquitin/fluorescence+ubiquitination+based+cell+cycle+indicator++fucci+/pm28245218-21-30-7
Average 90 stars, based on 1 article reviews
fluorescence ubiquitination-based cell-cycle indicator (fucci) - by Bioz Stars, 2026-09
90/100 stars
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fluorescently labelled ubiquitin  (LifeSensors)
90
LifeSensors fluorescently labelled ubiquitin
DUBs examined for behavior against ubiquitylated substrates were active enzymes. A. The fourteen DUBs examined in <xref ref-type=Fig. 2 all showed activity (signal versus time over 1 h) by CHOP reporter assay (LifeSensors, Inc.), except AMSH. B. AMSH was examined for activity (versus time over 1 h) against different substrates, di-ubiquitin substrates labeled with an internally quenched fluorophore pair. As expected, activity was specific to K63-linkage of the diubiquitin. C. E3Lite assay data (endpoint RFU signal) for triplicate wells either ubiquitylated with E3 mix (Praja1, Carp2, Murf1) and then mock treated with DUB (“No DUB”); mock ubiquitylated using reaction mixture lacking an E3 and then mock treated with DUB (“Not UB'd”); or ubiquitylated with E3 mix and then treated with DUB enzyme (as listed). " width="250" height="auto" />
Fluorescently Labelled Ubiquitin, supplied by LifeSensors, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ubiquitin/fluorescently+labelled+ubiquitin/pmc04965700__SC___007___C6SC01167E___s001-168-2-9
Average 90 stars, based on 1 article reviews
fluorescently labelled ubiquitin - by Bioz Stars, 2026-09
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fluorescent ubiquitination-based cell cycle indicator  (Future Medicine Ltd)
90
Future Medicine Ltd fluorescent ubiquitination-based cell cycle indicator
DUBs examined for behavior against ubiquitylated substrates were active enzymes. A. The fourteen DUBs examined in <xref ref-type=Fig. 2 all showed activity (signal versus time over 1 h) by CHOP reporter assay (LifeSensors, Inc.), except AMSH. B. AMSH was examined for activity (versus time over 1 h) against different substrates, di-ubiquitin substrates labeled with an internally quenched fluorophore pair. As expected, activity was specific to K63-linkage of the diubiquitin. C. E3Lite assay data (endpoint RFU signal) for triplicate wells either ubiquitylated with E3 mix (Praja1, Carp2, Murf1) and then mock treated with DUB (“No DUB”); mock ubiquitylated using reaction mixture lacking an E3 and then mock treated with DUB (“Not UB'd”); or ubiquitylated with E3 mix and then treated with DUB enzyme (as listed). " width="250" height="auto" />
Fluorescent Ubiquitination Based Cell Cycle Indicator, supplied by Future Medicine Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ubiquitin/fluorescent+ubiquitination+based+cell+cycle+indicator/pmc06094608-2-64-83
Average 90 stars, based on 1 article reviews
fluorescent ubiquitination-based cell cycle indicator - by Bioz Stars, 2026-09
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visualization ubiquitin conjugates ubiquitin-mediated fluorescence  (Cold Spring Harbor Laboratory Press)
90
Cold Spring Harbor Laboratory Press visualization ubiquitin conjugates ubiquitin-mediated fluorescence
DUBs examined for behavior against ubiquitylated substrates were active enzymes. A. The fourteen DUBs examined in <xref ref-type=Fig. 2 all showed activity (signal versus time over 1 h) by CHOP reporter assay (LifeSensors, Inc.), except AMSH. B. AMSH was examined for activity (versus time over 1 h) against different substrates, di-ubiquitin substrates labeled with an internally quenched fluorophore pair. As expected, activity was specific to K63-linkage of the diubiquitin. C. E3Lite assay data (endpoint RFU signal) for triplicate wells either ubiquitylated with E3 mix (Praja1, Carp2, Murf1) and then mock treated with DUB (“No DUB”); mock ubiquitylated using reaction mixture lacking an E3 and then mock treated with DUB (“Not UB'd”); or ubiquitylated with E3 mix and then treated with DUB enzyme (as listed). " width="250" height="auto" />
Visualization Ubiquitin Conjugates Ubiquitin Mediated Fluorescence, supplied by Cold Spring Harbor Laboratory Press, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ubiquitin/visualization+ubiquitin+conjugates+ubiquitin+mediated+fluorescence/pm24086058-3-12-87
Average 90 stars, based on 1 article reviews
visualization ubiquitin conjugates ubiquitin-mediated fluorescence - by Bioz Stars, 2026-09
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fluorescence-ubiquitin-based cell cycle indicator system  (BioResource International Inc)
90
BioResource International Inc fluorescence-ubiquitin-based cell cycle indicator system
DUBs examined for behavior against ubiquitylated substrates were active enzymes. A. The fourteen DUBs examined in <xref ref-type=Fig. 2 all showed activity (signal versus time over 1 h) by CHOP reporter assay (LifeSensors, Inc.), except AMSH. B. AMSH was examined for activity (versus time over 1 h) against different substrates, di-ubiquitin substrates labeled with an internally quenched fluorophore pair. As expected, activity was specific to K63-linkage of the diubiquitin. C. E3Lite assay data (endpoint RFU signal) for triplicate wells either ubiquitylated with E3 mix (Praja1, Carp2, Murf1) and then mock treated with DUB (“No DUB”); mock ubiquitylated using reaction mixture lacking an E3 and then mock treated with DUB (“Not UB'd”); or ubiquitylated with E3 mix and then treated with DUB enzyme (as listed). " width="250" height="auto" />
Fluorescence Ubiquitin Based Cell Cycle Indicator System, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+ubiquitin/fluorescence+ubiquitin+based+cell+cycle+indicator+system/pm22365624-22-21-14
Average 90 stars, based on 1 article reviews
fluorescence-ubiquitin-based cell cycle indicator system - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Chemical activation of the C terminus of ubiquitin as a thioester (e.g. UbMES or UbFluor) can bypass the need for E1, E2, and ATP, downsizing the 5-component native cascade reaction (E1, E2, ATP, PARKIN, and Ub) to 2 components (PARKIN and ByS probes). Transthiolation with UbMES releases a mercaptoethanesulfonate group (MES), whereas transthiolation with UbFluor releases fluorescein thiol.

Journal: The Journal of Biological Chemistry

Article Title: UbMES and UbFluor: Novel probes for ring-between-ring (RBR) E3 ubiquitin ligase PARKIN

doi: 10.1074/jbc.M116.773200

Figure Lengend Snippet: Chemical activation of the C terminus of ubiquitin as a thioester (e.g. UbMES or UbFluor) can bypass the need for E1, E2, and ATP, downsizing the 5-component native cascade reaction (E1, E2, ATP, PARKIN, and Ub) to 2 components (PARKIN and ByS probes). Transthiolation with UbMES releases a mercaptoethanesulfonate group (MES), whereas transthiolation with UbFluor releases fluorescein thiol.

Article Snippet: UbcH7∼FUb discharge assay UbcH7∼FUb was prepared by incubating Ube1 (0.5 μ m ), UbcH7 (10 μ m ), fluorescent ubiquitin (FUb, LifeSensors, 15 μ m ), and 2 m m ATP in 300 m m NaCl, 8 m m MgCl 2 , 50 m m Tris, pH 7.6, for 30 min at room temperature.

Techniques: Activation Assay, Ubiquitin Proteomics

DUBs examined for behavior against ubiquitylated substrates were active enzymes. A. The fourteen DUBs examined in <xref ref-type=Fig. 2 all showed activity (signal versus time over 1 h) by CHOP reporter assay (LifeSensors, Inc.), except AMSH. B. AMSH was examined for activity (versus time over 1 h) against different substrates, di-ubiquitin substrates labeled with an internally quenched fluorophore pair. As expected, activity was specific to K63-linkage of the diubiquitin. C. E3Lite assay data (endpoint RFU signal) for triplicate wells either ubiquitylated with E3 mix (Praja1, Carp2, Murf1) and then mock treated with DUB (“No DUB”); mock ubiquitylated using reaction mixture lacking an E3 and then mock treated with DUB (“Not UB'd”); or ubiquitylated with E3 mix and then treated with DUB enzyme (as listed). " width="100%" height="100%">

Journal: Biochimica et Biophysica Acta. Molecular Cell Research

Article Title: A microarray of ubiquitylated proteins for profiling deubiquitylase activity reveals the critical roles of both chain and substrate ☆

doi: 10.1016/j.bbamcr.2012.05.006

Figure Lengend Snippet: DUBs examined for behavior against ubiquitylated substrates were active enzymes. A. The fourteen DUBs examined in Fig. 2 all showed activity (signal versus time over 1 h) by CHOP reporter assay (LifeSensors, Inc.), except AMSH. B. AMSH was examined for activity (versus time over 1 h) against different substrates, di-ubiquitin substrates labeled with an internally quenched fluorophore pair. As expected, activity was specific to K63-linkage of the diubiquitin. C. E3Lite assay data (endpoint RFU signal) for triplicate wells either ubiquitylated with E3 mix (Praja1, Carp2, Murf1) and then mock treated with DUB (“No DUB”); mock ubiquitylated using reaction mixture lacking an E3 and then mock treated with DUB (“Not UB'd”); or ubiquitylated with E3 mix and then treated with DUB enzyme (as listed).

Article Snippet: Since this DUB is known to prefer K63 linked chains, we incubated it with a K63 linked internally quenched fluorescence based di-ubiquitin substrate (LifeSensors, Inc.).

Techniques: Activity Assay, Reporter Assay, Ubiquitin Proteomics, Labeling