fluorescein Search Results


qpcrbio sygreen mix  (PCR Biosystems Ltd)
97
PCR Biosystems Ltd qpcrbio sygreen mix
Qpcrbio Sygreen Mix, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/qpcrbio sygreen mix/product/PCR Biosystems Ltd
Average 97 stars, based on 1 article reviews
qpcrbio sygreen mix - by Bioz Stars, 2026-04
97/100 stars
  Buy from Supplier
fluorescein labeled sirna  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology fluorescein labeled sirna
FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the <t>siRNA-free</t> (only H2O) control. All data are the means S.E.
Fluorescein Labeled Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescein labeled sirna/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
fluorescein labeled sirna - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier
pentafluorobenzenesulfonyl fluorescein  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology pentafluorobenzenesulfonyl fluorescein
FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the <t>siRNA-free</t> (only H2O) control. All data are the means S.E.
Pentafluorobenzenesulfonyl Fluorescein, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pentafluorobenzenesulfonyl fluorescein/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
pentafluorobenzenesulfonyl fluorescein - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
goat anti chicken fitc  (AvesLabs)
98
AvesLabs goat anti chicken fitc
FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the <t>siRNA-free</t> (only H2O) control. All data are the means S.E.
Goat Anti Chicken Fitc, supplied by AvesLabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti chicken fitc/product/AvesLabs
Average 98 stars, based on 1 article reviews
goat anti chicken fitc - by Bioz Stars, 2026-04
98/100 stars
  Buy from Supplier
fda approved pis  (Biosynth Carbosynth)
93
Biosynth Carbosynth fda approved pis
FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the <t>siRNA-free</t> (only H2O) control. All data are the means S.E.
Fda Approved Pis, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fda approved pis/product/Biosynth Carbosynth
Average 93 stars, based on 1 article reviews
fda approved pis - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
fluorescein isothiocyanate fitc conjugated goat anti mouse secondary antibody  (Biosynth Carbosynth)
90
Biosynth Carbosynth fluorescein isothiocyanate fitc conjugated goat anti mouse secondary antibody
FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the <t>siRNA-free</t> (only H2O) control. All data are the means S.E.
Fluorescein Isothiocyanate Fitc Conjugated Goat Anti Mouse Secondary Antibody, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescein isothiocyanate fitc conjugated goat anti mouse secondary antibody/product/Biosynth Carbosynth
Average 90 stars, based on 1 article reviews
fluorescein isothiocyanate fitc conjugated goat anti mouse secondary antibody - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
fitc conjugated goat anti rabbit  (Jackson Immuno)
96
Jackson Immuno fitc conjugated goat anti rabbit
FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the <t>siRNA-free</t> (only H2O) control. All data are the means S.E.
Fitc Conjugated Goat Anti Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc conjugated goat anti rabbit/product/Jackson Immuno
Average 96 stars, based on 1 article reviews
fitc conjugated goat anti rabbit - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier
anti mouse antibody  (Jackson Immuno)
85
Jackson Immuno anti mouse antibody
FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the <t>siRNA-free</t> (only H2O) control. All data are the means S.E.
Anti Mouse Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mouse antibody/product/Jackson Immuno
Average 85 stars, based on 1 article reviews
anti mouse antibody - by Bioz Stars, 2026-04
85/100 stars
  Buy from Supplier
fitc goat anti mouse  (Jackson Immuno)
93
Jackson Immuno fitc goat anti mouse
FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the <t>siRNA-free</t> (only H2O) control. All data are the means S.E.
Fitc Goat Anti Mouse, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc goat anti mouse/product/Jackson Immuno
Average 93 stars, based on 1 article reviews
fitc goat anti mouse - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
alexafluor 488 donkey anti chicken  (Jackson Immuno)
93
Jackson Immuno alexafluor 488 donkey anti chicken
FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the <t>siRNA-free</t> (only H2O) control. All data are the means S.E.
Alexafluor 488 Donkey Anti Chicken, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexafluor 488 donkey anti chicken/product/Jackson Immuno
Average 93 stars, based on 1 article reviews
alexafluor 488 donkey anti chicken - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
goat anti mouse igg fitc conjugated  (Jackson Immuno)
92
Jackson Immuno goat anti mouse igg fitc conjugated
FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the <t>siRNA-free</t> (only H2O) control. All data are the means S.E.
Goat Anti Mouse Igg Fitc Conjugated, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti mouse igg fitc conjugated/product/Jackson Immuno
Average 92 stars, based on 1 article reviews
goat anti mouse igg fitc conjugated - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier
mouse f ab 2  (Jackson Immuno)
93
Jackson Immuno mouse f ab 2
FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the <t>siRNA-free</t> (only H2O) control. All data are the means S.E.
Mouse F Ab 2, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse f ab 2/product/Jackson Immuno
Average 93 stars, based on 1 article reviews
mouse f ab 2 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier

Image Search Results


FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the siRNA-free (only H2O) control. All data are the means S.E.

Journal: Journal of Biological Chemistry

Article Title: The Leukocyte Chemotactic Receptor FPR1 Is Functionally Expressed on Human Lens Epithelial Cells

doi: 10.1074/jbc.m112.411181

Figure Lengend Snippet: FIGURE 1. Lens epithelial cells constitutively express FPR1; RNA and immunophenotypic evidence. A, RNA is shown. FHL 124 cells nucleofected with the shRNA indicated on the x axis were analyzed for FPR1 mRNA expression by PCR. B, immunophenotype is shown. FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with a PE-labeled anti-FPR1 mAb. C, ligand binding; FHL 124 cells nucleofected with the shRNAs indicated on the x axis were analyzed by FACS with the fluorescent agonist ligand fNLFNYK-Fl (10 nM). D, Ca2 assay; FHL 124 cells nucleofected with the shRNAs indicated in the legend were stimulated with 1 M fMLF, and the Ca2 response was followed for 150 s. The base line (first 20 s) of each individual curve and a background curve recorded with only buffer (no fMLF) were subtracted from each signal. Then all experiments were pooled and transformed to % of the peak signal intensity of the siRNA-free (only H2O) control. All data are the means S.E.

Article Snippet: 100 pmol of FPR1 siRNA (three 20–25-nucleotide siRNAs, catalog no. sc-40121, Santa Cruz Biotechnology, Santa Cruz, CA), 100 pmol of scrambled fluorescein-labeled siRNA (Santa Cruz Biotechnology, catalog no. sc-36869), or water was added to 100 l of cell suspension and nucleofected with program X-005 (FHL 124) or Q-001 (HEK 293-FPR1 ) of the Nucleofector II Device (Lonza).

Techniques: shRNA, Expressing, Labeling, Ligand Binding Assay, Transformation Assay, Control