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Image Search Results
Journal: Carcinogenesis
Article Title: CHRNA5 as negative regulator of nicotine signaling in normal and cancer bronchial cells: effects on motility, migration and p63 expression
doi: 10.1093/carcin/bgr090
Figure Lengend Snippet: Effects of nicotine and antagonists on calcium influx in cells with silenced CHRNA5. Calcium influx in H1299 (A–D), 16HBE (E–F) and NHBE (G–E) was detected using the fluorescent marker Fluo-4. Panels A, C, E and G: microphographs of cells with artificial colors expressing Ca2+ levels. Color ranges from blue (low amount of Ca2+) to green, yellow and red (high amount of Ca2+). Panels B, D, F and G: quantitative analysis of Ca2+ influx in H1299 was performed using the Image J analysis program (http://rsbweb.nih.gov/ij/, Jan 2010). Nicotine was added at t = 0 sec (final concentration of nicotine: 1 mM) to cells transfected with siRNA CHRNA5 (A and B) or treated with α-conotoxin MII (Ctx, 10 nM) or α-bungarotoxin (Bgtx, 1 μM) (C–G).
Article Snippet: The
Techniques: Marker, Expressing, Concentration Assay, Transfection