Journal:

Article Title: Female X-chromosome mosaicism for gp91phox expression diversifies leukocyte responses during endotoxemia

doi: 10.1097/CCM.0b013e3181eb9ed6

Figure Lengend Snippet: Lipopolysaccharide (LPS)-induced changes in bone marrow (BM) and blood cell composition in WT, gp91phox-deficient and mosaic animals. Twenty-four hours after LPS or saline controls, BM cells and blood were collected and processed for flow cytometry analyses, as described in Materials and Methods. Graphs A and B depict BM myeloid cell content identified by CD45/CD11b double positive (A) and B-cell content identified by CD45/CD19 double positive staining (B). Graphs C–F depict the numbers of circulating CD11b+ (mostly neutrophils) and different T-cell (CD4+ and CD8+) and B-cell (CD19+) subsets as indicated. Cell numbers were calculated from percent distribution of cells and total white blood cell yield. Statistically significant difference (p < .05): *compared with control within the same genotype; #compared with WT or heterozygous controls. Mean ± SEM, n = 7–8 animals in each group.

Article Snippet: Flourochrome conjugated antibodies, assay diluents, lysing and permeabilizing flow cytometry solutions and kits were purchased from BD Biosiences (San Jose, CA) and BD Pharmingen (San Diego, CA).

Techniques: Flow Cytometry, Staining

Journal: Frontiers in Pharmacology

Article Title: Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells

doi: 10.3389/fphar.2019.00028

Figure Lengend Snippet: Effect of estradiol on cell cycle. Flow cytometry analysis of propidium iodide-labeled EPN (A) or CPEC (B) cells was done, as described in Methods. Quiescent cells were left under basal conditions or treated with estradiol (20 nM; E 2 ) for the indicated times. Histograms represent the % counts/FL2 areas of labeled cells in the different cell cycle phases after 24 or 48 h of hormonal treatment. Means and SEMs are shown. n represents the number of experiments. ∗ p < 0,05 for each experimental point vs. the corresponding untreated control.

Article Snippet: Permeabilization was performed by incubating 10 6 cells in 0,5 ml of flow cytometry analysis (FACS) permeabilizing solution, which contains RNAses and propidium iodide (Beckton Dickinson).

Techniques: Flow Cytometry, Labeling, Control

Journal: Frontiers in Pharmacology

Article Title: Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells

doi: 10.3389/fphar.2019.00028

Figure Lengend Snippet: Effect of both estradiol and pasireotide on cell cycle. Flow cytometry analysis of propidium iodide-labelled EPN (A) or CPEC (B) was done, as described in Methods. In panel (A,B) , quiescent cells were left under untreated or treated for 24 h with 0,1 μM pasireotide, in the absence or presence of 20 nM estradiol. Histograms in panel (A,B) represent the % counts/FL2 areas of labeled cells in the different cell cycle phases after the indicated treatments. Means and SEMs are shown. n represents the number of experiments. ∗ p < 0,05; ∗∗ p < 0,01. In panel (C) , quiescent EPN (left section) or CPEC (right section) cells were left untreated or treated for 24 h with 0,1 μM pasireotide, in the absence or presence of 20 nM estradiol. Lysate proteins were separated by SDS-PAGE, transferred to PDVF membrane and filters were then analyzed by Western blot, using the anti-activated caspase 3 or anti-tubulin antibodies.

Article Snippet: Permeabilization was performed by incubating 10 6 cells in 0,5 ml of flow cytometry analysis (FACS) permeabilizing solution, which contains RNAses and propidium iodide (Beckton Dickinson).

Techniques: Flow Cytometry, Labeling, SDS Page, Membrane, Western Blot