flow cytometric analysis Search Results


flow cytometry assays  (Merck KGaA)
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Merck KGaA flow cytometry assays
Flow Cytometry Assays, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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facsverse laser flow cytometric analysis system  (Becton Dickinson)
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Becton Dickinson facsverse laser flow cytometric analysis system
Facsverse Laser Flow Cytometric Analysis System, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometric analysis using facs caliber  (Becton Dickinson)
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Becton Dickinson flow cytometric analysis using facs caliber
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flow cytometric analysis facscan fl2 channel  (Becton Dickinson)
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Becton Dickinson flow cytometric analysis facscan fl2 channel
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flow cytometric analysis facscan  (Becton Dickinson)
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Becton Dickinson flow cytometric analysis facscan
Knockdown of PD-1, CTLA-4 or both decreases arginase I activity and expression. a PD-1 and CTLA4 siRNA reduced the level of PD-1 and CTLA-4, respectively, but not that of GAPDH transcript expression in the transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by RT-PCR. b PD-1, CTLA4 or both siRNA but not non-specific siRNA, reduced the level of surface PD-1 and CTLA4 expression in transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by flow <t>cytometric</t> analysis, respectively. B1, B2 Expression of PD-1 and CTLA-4 on the siRNA transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, respectively. Black line Isotype control, dark black line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected with PD-1 and CTLA-4 specific siRNA, respectively, dotted line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected by control mock siRNA. c Knockdown of PD-1, CTLA-4 or both reduced arginase I activity (C1) and level of transcription (C2). PD-1, CTLA-4 or both siRNA transfected PD1+CTLA-4+ MDSCs were cultured in the conditioned medium for 24 h and then lysed for analysis of arginase I activity. The total RNA was extracted for transcription assay according to the described protocol in “Materials and methods”. T. Sup. Conditioned medium containing 25% murine ovarian carcinoma supernatant (V/V), PD1siRNA, CTLA4siRNA and PD1+CTLA4siRNA, respectively, were PD1 siRNA, CTLA4 siRNA or both transfected PD1+CTLA4+MDSCs, Mock siRNA control siRNA transfected PD1+CTLA4+MDSCs
Flow Cytometric Analysis Facscan, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multicolor flow cytometry analysis  (Verlag GmbH)
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Verlag GmbH multicolor flow cytometry analysis
Knockdown of PD-1, CTLA-4 or both decreases arginase I activity and expression. a PD-1 and CTLA4 siRNA reduced the level of PD-1 and CTLA-4, respectively, but not that of GAPDH transcript expression in the transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by RT-PCR. b PD-1, CTLA4 or both siRNA but not non-specific siRNA, reduced the level of surface PD-1 and CTLA4 expression in transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by flow <t>cytometric</t> analysis, respectively. B1, B2 Expression of PD-1 and CTLA-4 on the siRNA transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, respectively. Black line Isotype control, dark black line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected with PD-1 and CTLA-4 specific siRNA, respectively, dotted line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected by control mock siRNA. c Knockdown of PD-1, CTLA-4 or both reduced arginase I activity (C1) and level of transcription (C2). PD-1, CTLA-4 or both siRNA transfected PD1+CTLA-4+ MDSCs were cultured in the conditioned medium for 24 h and then lysed for analysis of arginase I activity. The total RNA was extracted for transcription assay according to the described protocol in “Materials and methods”. T. Sup. Conditioned medium containing 25% murine ovarian carcinoma supernatant (V/V), PD1siRNA, CTLA4siRNA and PD1+CTLA4siRNA, respectively, were PD1 siRNA, CTLA4 siRNA or both transfected PD1+CTLA4+MDSCs, Mock siRNA control siRNA transfected PD1+CTLA4+MDSCs
Multicolor Flow Cytometry Analysis, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometrical analysis  (Becton Dickinson)
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Becton Dickinson flow cytometrical analysis
Knockdown of PD-1, CTLA-4 or both decreases arginase I activity and expression. a PD-1 and CTLA4 siRNA reduced the level of PD-1 and CTLA-4, respectively, but not that of GAPDH transcript expression in the transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by RT-PCR. b PD-1, CTLA4 or both siRNA but not non-specific siRNA, reduced the level of surface PD-1 and CTLA4 expression in transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by flow <t>cytometric</t> analysis, respectively. B1, B2 Expression of PD-1 and CTLA-4 on the siRNA transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, respectively. Black line Isotype control, dark black line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected with PD-1 and CTLA-4 specific siRNA, respectively, dotted line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected by control mock siRNA. c Knockdown of PD-1, CTLA-4 or both reduced arginase I activity (C1) and level of transcription (C2). PD-1, CTLA-4 or both siRNA transfected PD1+CTLA-4+ MDSCs were cultured in the conditioned medium for 24 h and then lysed for analysis of arginase I activity. The total RNA was extracted for transcription assay according to the described protocol in “Materials and methods”. T. Sup. Conditioned medium containing 25% murine ovarian carcinoma supernatant (V/V), PD1siRNA, CTLA4siRNA and PD1+CTLA4siRNA, respectively, were PD1 siRNA, CTLA4 siRNA or both transfected PD1+CTLA4+MDSCs, Mock siRNA control siRNA transfected PD1+CTLA4+MDSCs
Flow Cytometrical Analysis, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometry analyses  (CEM Corporation)
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CEM Corporation flow cytometry analyses
Knockdown of PD-1, CTLA-4 or both decreases arginase I activity and expression. a PD-1 and CTLA4 siRNA reduced the level of PD-1 and CTLA-4, respectively, but not that of GAPDH transcript expression in the transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by RT-PCR. b PD-1, CTLA4 or both siRNA but not non-specific siRNA, reduced the level of surface PD-1 and CTLA4 expression in transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by flow <t>cytometric</t> analysis, respectively. B1, B2 Expression of PD-1 and CTLA-4 on the siRNA transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, respectively. Black line Isotype control, dark black line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected with PD-1 and CTLA-4 specific siRNA, respectively, dotted line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected by control mock siRNA. c Knockdown of PD-1, CTLA-4 or both reduced arginase I activity (C1) and level of transcription (C2). PD-1, CTLA-4 or both siRNA transfected PD1+CTLA-4+ MDSCs were cultured in the conditioned medium for 24 h and then lysed for analysis of arginase I activity. The total RNA was extracted for transcription assay according to the described protocol in “Materials and methods”. T. Sup. Conditioned medium containing 25% murine ovarian carcinoma supernatant (V/V), PD1siRNA, CTLA4siRNA and PD1+CTLA4siRNA, respectively, were PD1 siRNA, CTLA4 siRNA or both transfected PD1+CTLA4+MDSCs, Mock siRNA control siRNA transfected PD1+CTLA4+MDSCs
Flow Cytometry Analyses, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lyoplate™ flow cytometric analysis  (Becton Dickinson)
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Becton Dickinson lyoplate™ flow cytometric analysis
Knockdown of PD-1, CTLA-4 or both decreases arginase I activity and expression. a PD-1 and CTLA4 siRNA reduced the level of PD-1 and CTLA-4, respectively, but not that of GAPDH transcript expression in the transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by RT-PCR. b PD-1, CTLA4 or both siRNA but not non-specific siRNA, reduced the level of surface PD-1 and CTLA4 expression in transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by flow <t>cytometric</t> analysis, respectively. B1, B2 Expression of PD-1 and CTLA-4 on the siRNA transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, respectively. Black line Isotype control, dark black line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected with PD-1 and CTLA-4 specific siRNA, respectively, dotted line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected by control mock siRNA. c Knockdown of PD-1, CTLA-4 or both reduced arginase I activity (C1) and level of transcription (C2). PD-1, CTLA-4 or both siRNA transfected PD1+CTLA-4+ MDSCs were cultured in the conditioned medium for 24 h and then lysed for analysis of arginase I activity. The total RNA was extracted for transcription assay according to the described protocol in “Materials and methods”. T. Sup. Conditioned medium containing 25% murine ovarian carcinoma supernatant (V/V), PD1siRNA, CTLA4siRNA and PD1+CTLA4siRNA, respectively, were PD1 siRNA, CTLA4 siRNA or both transfected PD1+CTLA4+MDSCs, Mock siRNA control siRNA transfected PD1+CTLA4+MDSCs
Lyoplate™ Flow Cytometric Analysis, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometric analysis  (Transgenic Rabbit Models)
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Transgenic Rabbit Models flow cytometric analysis
Knockdown of PD-1, CTLA-4 or both decreases arginase I activity and expression. a PD-1 and CTLA4 siRNA reduced the level of PD-1 and CTLA-4, respectively, but not that of GAPDH transcript expression in the transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by RT-PCR. b PD-1, CTLA4 or both siRNA but not non-specific siRNA, reduced the level of surface PD-1 and CTLA4 expression in transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by flow <t>cytometric</t> analysis, respectively. B1, B2 Expression of PD-1 and CTLA-4 on the siRNA transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, respectively. Black line Isotype control, dark black line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected with PD-1 and CTLA-4 specific siRNA, respectively, dotted line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected by control mock siRNA. c Knockdown of PD-1, CTLA-4 or both reduced arginase I activity (C1) and level of transcription (C2). PD-1, CTLA-4 or both siRNA transfected PD1+CTLA-4+ MDSCs were cultured in the conditioned medium for 24 h and then lysed for analysis of arginase I activity. The total RNA was extracted for transcription assay according to the described protocol in “Materials and methods”. T. Sup. Conditioned medium containing 25% murine ovarian carcinoma supernatant (V/V), PD1siRNA, CTLA4siRNA and PD1+CTLA4siRNA, respectively, were PD1 siRNA, CTLA4 siRNA or both transfected PD1+CTLA4+MDSCs, Mock siRNA control siRNA transfected PD1+CTLA4+MDSCs
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liquid counting beads (4.5 μm size) for flow cytometric analysis (335925)  (Becton Dickinson)
90
Becton Dickinson liquid counting beads (4.5 μm size) for flow cytometric analysis (335925)
Knockdown of PD-1, CTLA-4 or both decreases arginase I activity and expression. a PD-1 and CTLA4 siRNA reduced the level of PD-1 and CTLA-4, respectively, but not that of GAPDH transcript expression in the transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by RT-PCR. b PD-1, CTLA4 or both siRNA but not non-specific siRNA, reduced the level of surface PD-1 and CTLA4 expression in transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by flow <t>cytometric</t> analysis, respectively. B1, B2 Expression of PD-1 and CTLA-4 on the siRNA transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, respectively. Black line Isotype control, dark black line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected with PD-1 and CTLA-4 specific siRNA, respectively, dotted line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected by control mock siRNA. c Knockdown of PD-1, CTLA-4 or both reduced arginase I activity (C1) and level of transcription (C2). PD-1, CTLA-4 or both siRNA transfected PD1+CTLA-4+ MDSCs were cultured in the conditioned medium for 24 h and then lysed for analysis of arginase I activity. The total RNA was extracted for transcription assay according to the described protocol in “Materials and methods”. T. Sup. Conditioned medium containing 25% murine ovarian carcinoma supernatant (V/V), PD1siRNA, CTLA4siRNA and PD1+CTLA4siRNA, respectively, were PD1 siRNA, CTLA4 siRNA or both transfected PD1+CTLA4+MDSCs, Mock siRNA control siRNA transfected PD1+CTLA4+MDSCs
Liquid Counting Beads (4.5 μm Size) For Flow Cytometric Analysis (335925), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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annexin v/pi staining and flow cytometric analysis  (Becton Dickinson)
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Becton Dickinson annexin v/pi staining and flow cytometric analysis
Knockdown of PD-1, CTLA-4 or both decreases arginase I activity and expression. a PD-1 and CTLA4 siRNA reduced the level of PD-1 and CTLA-4, respectively, but not that of GAPDH transcript expression in the transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by RT-PCR. b PD-1, CTLA4 or both siRNA but not non-specific siRNA, reduced the level of surface PD-1 and CTLA4 expression in transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by flow <t>cytometric</t> analysis, respectively. B1, B2 Expression of PD-1 and CTLA-4 on the siRNA transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, respectively. Black line Isotype control, dark black line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected with PD-1 and CTLA-4 specific siRNA, respectively, dotted line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected by control mock siRNA. c Knockdown of PD-1, CTLA-4 or both reduced arginase I activity (C1) and level of transcription (C2). PD-1, CTLA-4 or both siRNA transfected PD1+CTLA-4+ MDSCs were cultured in the conditioned medium for 24 h and then lysed for analysis of arginase I activity. The total RNA was extracted for transcription assay according to the described protocol in “Materials and methods”. T. Sup. Conditioned medium containing 25% murine ovarian carcinoma supernatant (V/V), PD1siRNA, CTLA4siRNA and PD1+CTLA4siRNA, respectively, were PD1 siRNA, CTLA4 siRNA or both transfected PD1+CTLA4+MDSCs, Mock siRNA control siRNA transfected PD1+CTLA4+MDSCs
Annexin V/Pi Staining And Flow Cytometric Analysis, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Knockdown of PD-1, CTLA-4 or both decreases arginase I activity and expression. a PD-1 and CTLA4 siRNA reduced the level of PD-1 and CTLA-4, respectively, but not that of GAPDH transcript expression in the transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by RT-PCR. b PD-1, CTLA4 or both siRNA but not non-specific siRNA, reduced the level of surface PD-1 and CTLA4 expression in transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by flow cytometric analysis, respectively. B1, B2 Expression of PD-1 and CTLA-4 on the siRNA transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, respectively. Black line Isotype control, dark black line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected with PD-1 and CTLA-4 specific siRNA, respectively, dotted line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected by control mock siRNA. c Knockdown of PD-1, CTLA-4 or both reduced arginase I activity (C1) and level of transcription (C2). PD-1, CTLA-4 or both siRNA transfected PD1+CTLA-4+ MDSCs were cultured in the conditioned medium for 24 h and then lysed for analysis of arginase I activity. The total RNA was extracted for transcription assay according to the described protocol in “Materials and methods”. T. Sup. Conditioned medium containing 25% murine ovarian carcinoma supernatant (V/V), PD1siRNA, CTLA4siRNA and PD1+CTLA4siRNA, respectively, were PD1 siRNA, CTLA4 siRNA or both transfected PD1+CTLA4+MDSCs, Mock siRNA control siRNA transfected PD1+CTLA4+MDSCs

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Regulation of arginase I activity and expression by both PD-1 and CTLA-4 on the myeloid-derived suppressor cells

doi: 10.1007/s00262-008-0591-5

Figure Lengend Snippet: Knockdown of PD-1, CTLA-4 or both decreases arginase I activity and expression. a PD-1 and CTLA4 siRNA reduced the level of PD-1 and CTLA-4, respectively, but not that of GAPDH transcript expression in the transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by RT-PCR. b PD-1, CTLA4 or both siRNA but not non-specific siRNA, reduced the level of surface PD-1 and CTLA4 expression in transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, as assessed by flow cytometric analysis, respectively. B1, B2 Expression of PD-1 and CTLA-4 on the siRNA transfected ovarian carcinoma-associated PD-1+CTLA-4+MDSCs, respectively. Black line Isotype control, dark black line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected with PD-1 and CTLA-4 specific siRNA, respectively, dotted line expression of PD-1 (B1) and CTLA-4 (B2) on ovarian carcinoma-associated PD-1+CTLA-4+MDSCs transfected by control mock siRNA. c Knockdown of PD-1, CTLA-4 or both reduced arginase I activity (C1) and level of transcription (C2). PD-1, CTLA-4 or both siRNA transfected PD1+CTLA-4+ MDSCs were cultured in the conditioned medium for 24 h and then lysed for analysis of arginase I activity. The total RNA was extracted for transcription assay according to the described protocol in “Materials and methods”. T. Sup. Conditioned medium containing 25% murine ovarian carcinoma supernatant (V/V), PD1siRNA, CTLA4siRNA and PD1+CTLA4siRNA, respectively, were PD1 siRNA, CTLA4 siRNA or both transfected PD1+CTLA4+MDSCs, Mock siRNA control siRNA transfected PD1+CTLA4+MDSCs

Article Snippet: Cells were then washed twice, resuspended in PBS containing 1% paraformaldehyde and 1% FCS and kept at 4°C prior to flow cytometric analysis (FAScan, Becton Dickson).

Techniques: Knockdown, Activity Assay, Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Control, Cell Culture, Transcription Assay