flotillin 1 Search Results


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anti flotillin 1  (Novus Biologicals)
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flotilin 1  (Novus Biologicals)
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flotillin  (Novus Biologicals)
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FIGURE 3 | SEC qEV single 35 nm columns are optimal for separating CSF EVs. Pools of SEC fractions (Fxs): 1–5 (column void volume), 6–9, 10–13, and 14–17 were generated using either the qEV Single 35 nm or 70 nm columns. (A) Equal concentration loading of protein lysate (0.1 µg) from SEC pools immunoblotted for flotillin and CD81. (B) Equal volume loading of protein lysate (37 µL) from SEC pools immunoblotted for APOA1 and albumin. (C) Size and concentration histograms of Fxs 6–9 generated using either the qEV Single 35 nm (white) or 70 nm (black) columns acquired by tunable resistive pulse sensing (TRPS).
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antibodies against flotillin 1  (Novus Biologicals)
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FIGURE 3 | SEC qEV single 35 nm columns are optimal for separating CSF EVs. Pools of SEC fractions (Fxs): 1–5 (column void volume), 6–9, 10–13, and 14–17 were generated using either the qEV Single 35 nm or 70 nm columns. (A) Equal concentration loading of protein lysate (0.1 µg) from SEC pools immunoblotted for flotillin and CD81. (B) Equal volume loading of protein lysate (37 µL) from SEC pools immunoblotted for APOA1 and albumin. (C) Size and concentration histograms of Fxs 6–9 generated using either the qEV Single 35 nm (white) or 70 nm (black) columns acquired by tunable resistive pulse sensing (TRPS).
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FIGURE 3 | SEC qEV single 35 nm columns are optimal for separating CSF EVs. Pools of SEC fractions (Fxs): 1–5 (column void volume), 6–9, 10–13, and 14–17 were generated using either the qEV Single 35 nm or 70 nm columns. (A) Equal concentration loading of protein lysate (0.1 µg) from SEC pools immunoblotted for flotillin and CD81. (B) Equal volume loading of protein lysate (37 µL) from SEC pools immunoblotted for APOA1 and albumin. (C) Size and concentration histograms of Fxs 6–9 generated using either the qEV Single 35 nm (white) or 70 nm (black) columns acquired by tunable resistive pulse sensing (TRPS).

Journal: Frontiers in cell and developmental biology

Article Title: Differential Effects of APOE Genotype on MicroRNA Cargo of Cerebrospinal Fluid Extracellular Vesicles in Females With Alzheimer's Disease Compared to Males.

doi: 10.3389/fcell.2022.864022

Figure Lengend Snippet: FIGURE 3 | SEC qEV single 35 nm columns are optimal for separating CSF EVs. Pools of SEC fractions (Fxs): 1–5 (column void volume), 6–9, 10–13, and 14–17 were generated using either the qEV Single 35 nm or 70 nm columns. (A) Equal concentration loading of protein lysate (0.1 µg) from SEC pools immunoblotted for flotillin and CD81. (B) Equal volume loading of protein lysate (37 µL) from SEC pools immunoblotted for APOA1 and albumin. (C) Size and concentration histograms of Fxs 6–9 generated using either the qEV Single 35 nm (white) or 70 nm (black) columns acquired by tunable resistive pulse sensing (TRPS).

Article Snippet: The following antibodies were used for immunoblotting: albumin 1:1,000 (#4929, Cell Signaling Technology, Danvers, MA), APOA1 (12C8) 1:200 (sc-080551, Santa Cruz Biotechnology, Dallas, TX), APOE 1:2000 (50A-G1A, Academy Bio-medical Company, Inc., Houston, TX), AnnV 1: 5,000 (GTX103250, GeneTex, Irvine, CA), CD9 (C-4) 1:200 (sc13118, Santa Cruz Biotechnology), CD11b 1:1,000 (ab133357, Abcam, Cambridge, United Kingdom), CD63 1:1,000 (ab134045, Abcam), CD81 (B-11) 1:100 (sc-166029, Santa Cruz Biotechnology), flotillin 1:10,000 (ab133497, Abcam), GLAST 1:500 (NB100-1869, Novus Biologicals, Littleton, CO), NCAM1 1:125 (NBP2-38452, Novus Biologicals), SYP 1:1,000 (#36406, Cell Signaling Technology), TMEM119 (#66948-1-Ig, Proteintech, Rosemont, IL), and TSG101 1:1,000 (ab125011, Abcam).

Techniques: Generated, Concentration Assay, Tunable Resistive Pulse Sensing

FIGURE 5 | CSF EVs isolated by SEC are enriched for exosome and MV markers. (A) Equal volume loading of protein lysate (37 µL) from pools of SEC Fractions (Fxs): 1–5 (column void volume), 6–9, 10–13, and 14–17 stained for total protein, and immunoblotted for albumin, APOA1, and APOE. (B) Equal concentration loading of protein lysate (0.1 µg) from pools of Fxs 1–5, 6–9, 10–13, and 14–17 immunoblotted for CD9, CD63, CD81, flotillin, TSG101, AnnV, SYP, NCAM-1, GLAST, CD11b, and TMEM119. (C) Equal concentration loading of (1 µg) from pools of Fxs 1–5, 6–9, 10–13, and 14–17 immunoblotted for SYP and CD11b. Postmortem human cerebral cortex protein lysate (0.1 µg and 1 µg) was run as a positive control for each gel ((A-C): Brain).

Journal: Frontiers in cell and developmental biology

Article Title: Differential Effects of APOE Genotype on MicroRNA Cargo of Cerebrospinal Fluid Extracellular Vesicles in Females With Alzheimer's Disease Compared to Males.

doi: 10.3389/fcell.2022.864022

Figure Lengend Snippet: FIGURE 5 | CSF EVs isolated by SEC are enriched for exosome and MV markers. (A) Equal volume loading of protein lysate (37 µL) from pools of SEC Fractions (Fxs): 1–5 (column void volume), 6–9, 10–13, and 14–17 stained for total protein, and immunoblotted for albumin, APOA1, and APOE. (B) Equal concentration loading of protein lysate (0.1 µg) from pools of Fxs 1–5, 6–9, 10–13, and 14–17 immunoblotted for CD9, CD63, CD81, flotillin, TSG101, AnnV, SYP, NCAM-1, GLAST, CD11b, and TMEM119. (C) Equal concentration loading of (1 µg) from pools of Fxs 1–5, 6–9, 10–13, and 14–17 immunoblotted for SYP and CD11b. Postmortem human cerebral cortex protein lysate (0.1 µg and 1 µg) was run as a positive control for each gel ((A-C): Brain).

Article Snippet: The following antibodies were used for immunoblotting: albumin 1:1,000 (#4929, Cell Signaling Technology, Danvers, MA), APOA1 (12C8) 1:200 (sc-080551, Santa Cruz Biotechnology, Dallas, TX), APOE 1:2000 (50A-G1A, Academy Bio-medical Company, Inc., Houston, TX), AnnV 1: 5,000 (GTX103250, GeneTex, Irvine, CA), CD9 (C-4) 1:200 (sc13118, Santa Cruz Biotechnology), CD11b 1:1,000 (ab133357, Abcam, Cambridge, United Kingdom), CD63 1:1,000 (ab134045, Abcam), CD81 (B-11) 1:100 (sc-166029, Santa Cruz Biotechnology), flotillin 1:10,000 (ab133497, Abcam), GLAST 1:500 (NB100-1869, Novus Biologicals, Littleton, CO), NCAM1 1:125 (NBP2-38452, Novus Biologicals), SYP 1:1,000 (#36406, Cell Signaling Technology), TMEM119 (#66948-1-Ig, Proteintech, Rosemont, IL), and TSG101 1:1,000 (ab125011, Abcam).

Techniques: Isolation, Staining, Concentration Assay, Positive Control