Journal:

Article Title: AP1 Regulation of Proliferation and Initiation of Apoptosis in Erythropoietin-Dependent Erythroid Cells

doi:

Figure Lengend Snippet: The presence of a dominant negative AP1 mutant blocks apoptosis in HCD57 cells. (A) Western blot analysis of 40 μg of nuclear protein from HCD57, HCD57-TAM-67, and HCD57 cells transfected with vector alone (HCD57-NEOR), using an anti-c-Jun antibody. Upper and lower arrows indicate the presence of the c-Jun and TAM-67 proteins, respectively. (b and c) Growth and survival properties of HCD57 and HCD57-TAM-67 cells exposed to EPO. (b) Proliferative response of HCD57 and HCD57-TAM-67 cells to EPO. Cells were cultured in the absence or presence of 1 U of EPO/ml. Data are indicated as percentages of the starting number of cells. ⧫, HCD57 cells cultured in EPO; ▴, HCD57-TAM-67 cells cultured in EPO; ▪, HCD57 cells cultured in the absence of EPO; ×, HCD57-TAM-67 cells cultured in the absence of EPO for 0, 24, 48, 72, and 96 h. Data are indicated as percentages of the starting number of cells. (d) Prevention of apoptosis by TAM-67 in HCD57 cells. Cells were cultured in the presence of EPO (lanes A to C and G to I) or in the absence of cytokine (lanes D to F and J to L) for the number of hours indicated. Ten micrograms of genomic DNA from HCD57 (lanes A to F) and HCD57-TAM-67 (lanes G to L) cells was resolved on a 2.25% agarose gel. Arrow indicates 100-bp marker.

Article Snippet: The presence of the TAM-67 message was verified by Northern blot analysis, and protein expression was verified by Western blot analysis using an anti-c-Jun antibody (Santa Cruz Biotechnology).

Techniques: Dominant Negative Mutation, Mutagenesis, Western Blot, Transfection, Plasmid Preparation, Cell Culture, Agarose Gel Electrophoresis, Marker

Journal:

Article Title: AP1 Regulation of Proliferation and Initiation of Apoptosis in Erythropoietin-Dependent Erythroid Cells

doi:

Figure Lengend Snippet: Bcl-XL levels are maintained in EPO-deprived HCD57-TAM-67 cells. Total cellular proteins were isolated from HCD57 and HCD57-TAM-67 cells and were subjected to Western blot analysis with an anti-Bcl-XL antibody. Positions of molecular weight markers are indicated at the left. Arrows indicate the presence of the Bcl-XL protein. Bcl-XL levels decreased 72 h following EPO withdrawal in HCD57 cells (top) but were unchanged in HCD57-TAM-67 cells deprived of EPO for 96 h (bottom).

Article Snippet: The presence of the TAM-67 message was verified by Northern blot analysis, and protein expression was verified by Western blot analysis using an anti-c-Jun antibody (Santa Cruz Biotechnology).

Techniques: Isolation, Western Blot, Molecular Weight