Journal: Therapeutic Advances in Chronic Disease

Article Title: Flibanserin for hypoactive sexual desire disorder: place in therapy

doi: 10.1177/2040622316679933

Figure Lengend Snippet: Structure of Flibanserin. Sprout Pharmaceuticals Inc., 2015.

Article Snippet: [ PubMed ] Sprout Pharmaceuticals Inc (2015) Addyi (Flibanserin) Tablets, for Oral Use: Us Prescribing Information 2015 [Online] .

Techniques:

Journal: Therapeutic Advances in Chronic Disease

Article Title: Flibanserin for hypoactive sexual desire disorder: place in therapy

doi: 10.1177/2040622316679933

Figure Lengend Snippet: Mean + SD Plasma Flibanserin Concentration-Time Profiles in Healthy Female Subjects Following a Single Oral Dose of 100mg of Flibanserin (Linear Scale). Sprout Pharmaceuticals Inc., 2015.

Article Snippet: [ PubMed ] Sprout Pharmaceuticals Inc (2015) Addyi (Flibanserin) Tablets, for Oral Use: Us Prescribing Information 2015 [Online] .

Techniques: Clinical Proteomics, Concentration Assay

Journal: Molecules

Article Title: Development and Greenness Evaluation of Spectrofluorometric Methods for Flibanserin Determination in Dosage Form and Human Urine Samples

doi: 10.3390/molecules25214932

Figure Lengend Snippet: Chemical structure of flibanserin.

Article Snippet: Hikma Pharmaceutical Industries (Cairo, Egypt) kindly supplied the study with flibanserin of purity 99.98% and Veroxeserin ® tablets (Batch 001) (each tablet contained 100-mg FB).

Techniques:

Journal: Molecules

Article Title: Development and Greenness Evaluation of Spectrofluorometric Methods for Flibanserin Determination in Dosage Form and Human Urine Samples

doi: 10.3390/molecules25214932

Figure Lengend Snippet: ( A ) Flibanserin (FB) excitation spectrum, ( B ) FB emission spectrum, ( C ) emission spectrum of urine as the blank and ( D ) emission spectrum of ethanol as the blank.

Article Snippet: Hikma Pharmaceutical Industries (Cairo, Egypt) kindly supplied the study with flibanserin of purity 99.98% and Veroxeserin ® tablets (Batch 001) (each tablet contained 100-mg FB).

Techniques:

Journal: Molecules

Article Title: Development and Greenness Evaluation of Spectrofluorometric Methods for Flibanserin Determination in Dosage Form and Human Urine Samples

doi: 10.3390/molecules25214932

Figure Lengend Snippet: Effect of the solvents on the relative fluorescence intensity of flibanserin (FB).

Article Snippet: Hikma Pharmaceutical Industries (Cairo, Egypt) kindly supplied the study with flibanserin of purity 99.98% and Veroxeserin ® tablets (Batch 001) (each tablet contained 100-mg FB).

Techniques: Fluorescence, Solvent

Journal: Therapeutic Advances in Chronic Disease

Article Title: Flibanserin for hypoactive sexual desire disorder: place in therapy

doi: 10.1177/2040622316679933

Figure Lengend Snippet: Structure of Flibanserin. Sprout Pharmaceuticals Inc., 2015.

Article Snippet: Taking flibanserin with food was noted to increase the amount absorbed by slowing the rate of transit [ Sprout Pharmaceuticals Inc, 2015 ].

Techniques:

Journal: Therapeutic Advances in Chronic Disease

Article Title: Flibanserin for hypoactive sexual desire disorder: place in therapy

doi: 10.1177/2040622316679933

Figure Lengend Snippet: Mean + SD Plasma Flibanserin Concentration-Time Profiles in Healthy Female Subjects Following a Single Oral Dose of 100mg of Flibanserin (Linear Scale). Sprout Pharmaceuticals Inc., 2015.

Article Snippet: Taking flibanserin with food was noted to increase the amount absorbed by slowing the rate of transit [ Sprout Pharmaceuticals Inc, 2015 ].

Techniques: Clinical Proteomics, Concentration Assay

Journal:

Article Title: Flibanserin, a potential antidepressant drug, lowers 5-HT and raises dopamine and noradrenaline in the rat prefrontal cortex dialysate: role of 5-HT 1A receptors

doi: 10.1038/sj.bjp.0705341

Figure Lengend Snippet: Extracellular 5-HT in the prefrontal cortex (PFC), dorsal raphe (DR) and ventral hippocampus (VH) of rats given 3 and 10 mg kg−1 flibanserin or vehicle intraperitoneally. Mean±s.e.m. of 5–7 rats. The arrows indicate the time of drug or vehicle injection. Solid symbols indicate P<0.05 vs basal values (Tukey–Kramer's test).

Article Snippet: Drug treatment Flibanserin (previously BIMT17; 1-[2-[4-(3-trifluoromethylphenyl)piperazin-1-yl] ethyl] benzimidazol-[1 H]-2-one) (Boehringer-Ingelheim, Milan, Italy) was dissolved in a vehicle containing 250 ml l −1 polyethylene glycol-400 and 22.7 ml l −1 .

Techniques: Injection

Journal:

Article Title: Flibanserin, a potential antidepressant drug, lowers 5-HT and raises dopamine and noradrenaline in the rat prefrontal cortex dialysate: role of 5-HT 1A receptors

doi: 10.1038/sj.bjp.0705341

Figure Lengend Snippet: Extracellular DA and NA in the prefrontal cortex (PFC) of rats given 3 and 10 mg kg−1 flibanserin or vehicle intraperitoneally. Mean±s.e.m. of 5–6 rats. The arrows indicate the time of drug or vehicle injection. Solid symbols indicate P<0.05 vs basal values (Tukey–Kramer's test).

Article Snippet: Drug treatment Flibanserin (previously BIMT17; 1-[2-[4-(3-trifluoromethylphenyl)piperazin-1-yl] ethyl] benzimidazol-[1 H]-2-one) (Boehringer-Ingelheim, Milan, Italy) was dissolved in a vehicle containing 250 ml l −1 polyethylene glycol-400 and 22.7 ml l −1 .

Techniques: Injection

Journal:

Article Title: Flibanserin, a potential antidepressant drug, lowers 5-HT and raises dopamine and noradrenaline in the rat prefrontal cortex dialysate: role of 5-HT 1A receptors

doi: 10.1038/sj.bjp.0705341

Figure Lengend Snippet: Effect of flibanserin alone and in combination with the selective 5-HT1A autoreceptor antagonist WAY100,635 on extracellular 5-HT in the prefrontal cortex. Rats were pretreated subcutaneously with saline (SAL) or 0.3 mg kg−1 WAY100,635 (WAY; first arrow). After 30 min, they received 10 mg kg−1 flibanserin (FLIB) or vehicle (VEH) intraperitoneally (second arrow) and extracellular 5-HT was measured for 2 h. Mean±s.e.m. of 3–6 rats. Solid symbols indicate P<0.05 vs basal values (Tukey–Kramer's test).

Article Snippet: Drug treatment Flibanserin (previously BIMT17; 1-[2-[4-(3-trifluoromethylphenyl)piperazin-1-yl] ethyl] benzimidazol-[1 H]-2-one) (Boehringer-Ingelheim, Milan, Italy) was dissolved in a vehicle containing 250 ml l −1 polyethylene glycol-400 and 22.7 ml l −1 .

Techniques: Saline

Journal:

Article Title: Flibanserin, a potential antidepressant drug, lowers 5-HT and raises dopamine and noradrenaline in the rat prefrontal cortex dialysate: role of 5-HT 1A receptors

doi: 10.1038/sj.bjp.0705341

Figure Lengend Snippet: Effect of flibanserin alone and in combination with the selective 5-HT1A autoreceptor antagonist WAY100,635 on extracellular DA and NA in the prefrontal cortex. Rats were injected subcutaneously with saline (SAL) or 0.3 mg kg−1 WAY100,635 (WAY; first arrow) and 30 min later received 10 mg kg−1 flibanserin (FLIB) or vehicle (VEH) intraperitoneally (second arrow); extracellular DA and NA were measured for 2 h. Mean±s.e.m. of 2–6 rats. Solid symbols indicate P<0.05 vs basal values (Tukey–Kramer's test).

Article Snippet: Drug treatment Flibanserin (previously BIMT17; 1-[2-[4-(3-trifluoromethylphenyl)piperazin-1-yl] ethyl] benzimidazol-[1 H]-2-one) (Boehringer-Ingelheim, Milan, Italy) was dissolved in a vehicle containing 250 ml l −1 polyethylene glycol-400 and 22.7 ml l −1 .

Techniques: Injection, Saline

Journal: Pharmaceuticals

Article Title: Cardioprotective Effect of Flibanserin against Isoproterenol-Induced Myocardial Infarction in Female Rats: Role of Cardiac 5-HT2A Receptor Gene/5-HT/Ca 2+ Pathway

doi: 10.3390/ph16040502

Figure Lengend Snippet: The ECG examination in different treated groups. ( a ): The electrocardiographic effect of FLP in different doses on ISO-induced MI in rats. ( b ): ECG in experimental rats pretreated with FLP (15, 30, and 45 mg/kg) in an ISO-induced MI model on HR, QT interval, R amplitude, and ST segment amplitude. ( A ) Normal group, ( B ) Iso group, ( C ) FLB 15 mg + Iso group, ( D ) FLB 30 mg + Iso group and FLB 45 mg + Iso group. Bars with different letters are significantly different according to DMRT at p < 0.001 level ( n = 5). Data are expressed as Mean ± SD. ECG = electrocardiogram, FLP = flibanserin, ISO = isoproterenol, MI = myocardial infarction, HR = heart rate, DMRT = Duncan’s multiple range test.

Article Snippet: Flibanserin was purchased from Rameda (Giza, Egypt) and was suspended in water.

Techniques:

Journal: Pharmaceuticals

Article Title: Cardioprotective Effect of Flibanserin against Isoproterenol-Induced Myocardial Infarction in Female Rats: Role of Cardiac 5-HT2A Receptor Gene/5-HT/Ca 2+ Pathway

doi: 10.3390/ph16040502

Figure Lengend Snippet: Histopathological examination in the different treated groups. ( A ): Photomicrograph for specimens from the heart stained with H&E 10× & 40× in different treated groups. In the normal group: [H&E 10×] shows Compact, uniformly arranged myocardial fibers (Black arrows), [H&E 40×] myocytes show preserved uniform nuclei (Black arrows) and cytoplasmic cross striation (Red arrows). In ISO group: [H&E 10×] shows a wide separation of myocardial fibers due to interstitial edema (Black arrows), areas of myocardial fibers loss of eosinophilic staining and becoming pallor in appearance (Arrowheads), the influx of inflammatory cells into myocardial fibers (Red arrows), [H&E 40×] shows pathological changes of MI, interstitial edema between myocardial fibers (Black arrows), loss of cytoplasmic striation of myocytes with shrinkage of nuclei and prominence of the cell membrane (Arrowheads), some myocytes show an absence of nuclei (Yellow arrows), the influx of macrophages, indicating an inflammatory response to remove dead fibers (Red arrows). In FLP (15 mg/kg) + ISO group: [H&E 10×] shows no significant difference when compared to the ISO group as there is still a wide separation of myocardial fibers due to interstitial edema (Black arrows), the influx of inflammatory cells into myocardial fibers (Red arrows), [H&E 40×] shows persistent pathological changes of MI, interstitial edema between myocardial fibers (Black arrows), loss of cytoplasmic striation of myocytes with shrinkage of nuclei and prominence of the cell membrane (Arrowheads), some myocytes show an absence of nuclei (Yellow arrows), the influx of macrophages indicating an inflammatory response to remove dead fibers (Red arrows). In FLP (30 mg/kg) + ISO group: [H&E 10×] still shows interstitial edema (Black arrows); however, a slight reduction in inflammatory cell influx is noticed (Red arrow), [H&E 40×] shows persistent pathological changes of MI, yet to a milder degree: interstitial edema between myocardial fibers (Black arrows), loss of cytoplasmic striation of few myocytes with shrinkage of nuclei and prominence of the cell membrane (Arrowheads), absence of nuclei in few myocytes (Yellow arrows), reduction in macrophages influx (Red arrows). In FLP (45 mg/kg) + ISO group: [H&E 10×] shows compact uniformly arranged myocardial fibers and absence of interstitial edema (Black arrows); few areas of chronic inflammatory cells are seen (Red arrow), [H&E 40×] myocytes show uniform nuclei and eosinophilic cytoplasm (Black arrows), few macrophages are seen (Red arrows). ( B ): histopathologic score in experimental rats pretreated with FLP (15, 30, and 45 mg/kg) in an ISO-induced MI model. Data were analyzed using Kruskal-Wallis followed by Dunn’s test for multiple comparisons against the normal group at p < 0.05., ** p < 0.01, *** p < 0.001, n = 6. H&E = hematoxylin and eosin, FLP = flibanserin, ISO = isoproterenol, MI = myocardial infarction.

Article Snippet: Flibanserin was purchased from Rameda (Giza, Egypt) and was suspended in water.

Techniques: Staining, Membrane

Journal: Pharmaceuticals

Article Title: Cardioprotective Effect of Flibanserin against Isoproterenol-Induced Myocardial Infarction in Female Rats: Role of Cardiac 5-HT2A Receptor Gene/5-HT/Ca 2+ Pathway

doi: 10.3390/ph16040502

Figure Lengend Snippet: Serum cardiac enzymes levels in experimental rats pretreated with FLP (15, 30, and 45 mg/kg) in an ISO-induced MI model ( A ): The cardiac serum level of Tnl ( B ): The cardiac serum level of CK-MB, ( C ): The cardiac serum level of LDH. ( D ): The cardiac serum level of CK. Bars with different letters are significantly different according to DMRT at p < 0.001 level ( n = 5). Data are expressed as Mean ± SD. FLP = flibanserin, ISO = isoproterenol, MI = myocardial infarction, Tnl = troponin I, CK-MB = creatinine kinase-MB, LDH = lactate dehydrogenase, CK = creatine kinase, DMRT = Duncan’s multiple range test.

Article Snippet: Flibanserin was purchased from Rameda (Giza, Egypt) and was suspended in water.

Techniques:

Journal: Pharmaceuticals

Article Title: Cardioprotective Effect of Flibanserin against Isoproterenol-Induced Myocardial Infarction in Female Rats: Role of Cardiac 5-HT2A Receptor Gene/5-HT/Ca 2+ Pathway

doi: 10.3390/ph16040502

Figure Lengend Snippet: Effect of FLP in different doses on heart tissue level of GSH and MDA. ( A ): Heart level of GSH in experimental rats pretreated with FLP (15, 30, and 45 mg/kg) in an ISO-induced MI model. ( B ) Heart level of MDA in experimental rats pretreated with FLP (15, 30, and 45 mg/kg) in an ISO-induced MI model. Bars with different letters are significantly different according to DMRT at p < 0.001 level ( n = 5). Data are expressed as Mean ± SD. FLP = flibanserin, GSH = reduced glutathione, MDA = malondialdehyde, ISO = isoproterenol, DMRT = Duncan’s multiple range test.

Article Snippet: Flibanserin was purchased from Rameda (Giza, Egypt) and was suspended in water.

Techniques:

Journal: Pharmaceuticals

Article Title: Cardioprotective Effect of Flibanserin against Isoproterenol-Induced Myocardial Infarction in Female Rats: Role of Cardiac 5-HT2A Receptor Gene/5-HT/Ca 2+ Pathway

doi: 10.3390/ph16040502

Figure Lengend Snippet: Proposed FLP action on DA, NE, and 5-HT centers of the brain. The figure represents the dopaminergic system ( A ) before and ( B ) after the administration of flibanserin. While noradrenergic system ( C ) before and ( D ) after administration of flibanserin. Finally, serotonergic system ( E ) before and ( F ) after administration of flibanserin. Glu = Glutamate, GABA = Gamma-aminobutyric acid, NE = Norepinephrine, DA = Dopamine, 5-HT = Serotonin, FLP = Flibanserin.

Article Snippet: Flibanserin was purchased from Rameda (Giza, Egypt) and was suspended in water.

Techniques: