Journal: PLoS Neglected Tropical Diseases

Article Title: Therapeutic efficacy of albendazole against soil-transmitted helminthiasis in children measured by five diagnostic methods

doi: 10.1371/journal.pntd.0007471

Figure Lengend Snippet: Equivalence of ALB drug efficacy against Ascaris lumbricoides measured by five diagnostic methods. Mean intensity of infection corresponds with the mean fecal egg counts (FEC; expressed as eggs per gram of stool (EPG)) for single (1x KK) and duplicate (2x KK) Kato-Katz, Mini-FLOTAC and FECPAK G2 and with the mean DNA concentration (expressed as genome equivalents per ml of DNA (GE/ml)) for qPCR. For A . lumbricoides , there is significant evidence that a diagnostic method results in equivalent drug efficacy results compared to a 1x KK when the 95% confidence intervals (95%CI) around the difference in drug efficacy does not include +/-2.5%. Significant evidence of equal drug efficacy results is indicated by ‘*’. For A . lumbricoides , drug efficacy was classified as ‘satisfactory’ when drug efficacy ≥95%, reduced when drug efficacy was less than 85% or doubtful in all other cases.

Article Snippet: For FECPAK G2 (κ Fleiss = 0.65, p = 0.01) there was a substantial agreement (0.61 ≤ κ Fleiss <0.81), for MiniFLOTAC (κ Fleiss = 0.60, p = 0.03) there was a moderate agreement (0.41 ≤ κ Fleiss <0.61).

Techniques: Diagnostic Assay, Infection, Concentration Assay

Journal: PLoS Neglected Tropical Diseases

Article Title: Therapeutic efficacy of albendazole against soil-transmitted helminthiasis in children measured by five diagnostic methods

doi: 10.1371/journal.pntd.0007471

Figure Lengend Snippet: Equivalence of drug efficacy of albendazole against hookworm infections measured by five diagnostic methods. Mean intensity of infection corresponds with the mean fecal egg counts (FEC; expressed as eggs per gram of stool (EPG)) for a single (1x KK) and duplicate (2x KK) Kato-Katz, Mini-FLOTAC and FECPAK G2 and with the mean DNA concentration (expressed as genome equivalents per ml of DNA (GE/ml)) for qPCR. For hookworm, there is significant evidence that a diagnostic method results in equivalent drug efficacy results compared to a 1x KK when the 95% confidence intervals (95%CI) around the difference in drug efficacy does not include +/- 5%. Significant evidence of equal drug efficacy results is indicated by ‘*’. For hookworm, drug efficacy was classified as ‘satisfactory’ when drug efficacy ≥90%, reduced when drug efficacy was less than 80% or doubtful in all other cases.

Article Snippet: For FECPAK G2 (κ Fleiss = 0.65, p = 0.01) there was a substantial agreement (0.61 ≤ κ Fleiss <0.81), for MiniFLOTAC (κ Fleiss = 0.60, p = 0.03) there was a moderate agreement (0.41 ≤ κ Fleiss <0.61).

Techniques: Diagnostic Assay, Infection, Concentration Assay

Journal: PLoS Neglected Tropical Diseases

Article Title: Therapeutic efficacy of albendazole against soil-transmitted helminthiasis in children measured by five diagnostic methods

doi: 10.1371/journal.pntd.0007471

Figure Lengend Snippet: Equivalence of drug efficacy of albendazole against Trichuris trichiura measured by five diagnostic methods. Mean intensity of infection corresponds with the mean fecal egg counts (FEC; expressed as eggs per gram of stool (EPG)) for single (1x KK) and duplicate (2x KK) Kato-Katz, Mini-FLOTAC and FECPAK G2 and with the mean DNA concentration (expressed as genome equivalents per ml of DNA (GE/ml)) for qPCR. For T . trichiura , there is significant evidence that a diagnostic method results in equivalent drug efficacy results compared to a 1x KK when the 95% confidence intervals (95%CI) around the difference in drug efficacy does not include +/-10%. Significant evidence of equal drug efficacy results is indicated by ‘*’. For T . trichiura , drug efficacy was classified as ‘satisfactory’ when drug efficacy ≥50%, reduced when drug efficacy was less than 40% or doubtful in all other cases.

Article Snippet: For FECPAK G2 (κ Fleiss = 0.65, p = 0.01) there was a substantial agreement (0.61 ≤ κ Fleiss <0.81), for MiniFLOTAC (κ Fleiss = 0.60, p = 0.03) there was a moderate agreement (0.41 ≤ κ Fleiss <0.61).

Techniques: Diagnostic Assay, Infection, Concentration Assay

Journal: PLoS Neglected Tropical Diseases

Article Title: Therapeutic efficacy of albendazole against soil-transmitted helminthiasis in children measured by five diagnostic methods

doi: 10.1371/journal.pntd.0007471

Figure Lengend Snippet: Agreement in classifying the efficacy of albendazole between single Kato-Katz and duplicate Kato-Katz, Mini-FLOTAC, FECPAK G2 and qPCR. The cross tables represent the agreement in classifying the efficacy of albendazole as ‘satisfactory’, ‘doubtful’ or ‘reduced’, between a single Kato-Katz (1x KK) and a duplicate Kato-Katz (2x KK), Mini-FLOTAC, FECPAK G2 and qPCR across the three study sites and for each soil-transmitted helminth species (9 observations). Drug efficacy is based on egg reduction rate (ERR) for microscopic methods and by means of reduction in genome equivalents (GERR) for qPCR. World Health Organization criteria to define drug efficacy into ‘satisfactory’, ‘doubtful’ and ‘reduced’ were previously recommended for a single Kato Katz (see Table 2 ).

Article Snippet: For FECPAK G2 (κ Fleiss = 0.65, p = 0.01) there was a substantial agreement (0.61 ≤ κ Fleiss <0.81), for MiniFLOTAC (κ Fleiss = 0.60, p = 0.03) there was a moderate agreement (0.41 ≤ κ Fleiss <0.61).

Techniques:

Journal: PLoS Neglected Tropical Diseases

Article Title: Therapeutic efficacy of albendazole against soil-transmitted helminthiasis in children measured by five diagnostic methods

doi: 10.1371/journal.pntd.0007471

Figure Lengend Snippet: The upper three bar plots illustrate the different classifications of individual albendazole efficacy for A . lumbricoides detected in the study population (n = 441; Ethiopia: 137, Lao PDR: 111, Pemba (Tanzania): 193) in each of the 3 countries using 5 different diagnostic methods (single (1x KK) and duplicate Kato-Katz (2x KK), Mini-FLOTAC (MF), FECPAK G2 (FP) and qPCR). The bars represent the number of cases. The color of the bars reflects the individual egg or genome equivalent reduction rate (i(G)ERR). The bottom three bar plots represent the classification of the i(G)ERRs for A . lumbricoides for those individuals for which i(G)ERR data was available for all five diagnostic methods (n = 258; Ethiopia: 80, Lao PDR: 50, Pemba (Tanzania): 128).

Article Snippet: For FECPAK G2 (κ Fleiss = 0.65, p = 0.01) there was a substantial agreement (0.61 ≤ κ Fleiss <0.81), for MiniFLOTAC (κ Fleiss = 0.60, p = 0.03) there was a moderate agreement (0.41 ≤ κ Fleiss <0.61).

Techniques: Diagnostic Assay

Journal: PLoS Neglected Tropical Diseases

Article Title: Therapeutic efficacy of albendazole against soil-transmitted helminthiasis in children measured by five diagnostic methods

doi: 10.1371/journal.pntd.0007471

Figure Lengend Snippet: The upper three bar plots illustrate the different classifications of individual ALB efficacy for hookworm detected in the study population (n = 457; Ethiopia: 90, Lao PDR: 228, Pemba, Tanzania: 139) in each of the 3 countries using 5 different diagnostic methods (single (1x KK) and duplicate Kato-Katz (2x KK), Mini-FLOTAC (MF), FECPAK G2 (FP) and qPCR). The bars represent the number of cases. The color of the bars reflects the individual egg or genome equivalent reduction rate (i(G)ERR). The bottom three bar plots represent the classification of the i(G)ERRs for hookworm for those individuals for which i(G)ERR data was available for all five diagnostic methods (n = 256; Ethiopia: 34, Lao PDR: 166, Pemba, Tanzania: 56).

Article Snippet: For FECPAK G2 (κ Fleiss = 0.65, p = 0.01) there was a substantial agreement (0.61 ≤ κ Fleiss <0.81), for MiniFLOTAC (κ Fleiss = 0.60, p = 0.03) there was a moderate agreement (0.41 ≤ κ Fleiss <0.61).

Techniques: Diagnostic Assay