flag yap1 Search Results


human yap1 gene orf cdna clone expression plasmid, c-flag tag  (Sino Biological)
N/A
Full length Clone DNA of Human Yes associated protein 1
   Buy from Supplier
human yap1  (Addgene inc)
94
Addgene inc human yap1
FIGURE 9 SIRT6 deacetylates <t>YAP1</t> at multiple lysine residues. (A) A diagram of human YAP1 domain structure and several known acetylated lysine residues. (B) Real-time PCR analysis of CYR61, CTGF, and ANKRD1 mRNAs in the LX-2 cells transfected with either WT or mutant human YAP1 plasmids in the presence of 5 ng/ml TGF-β1 (n = 3). (C) YAP1 acetylation analysis in LX-2 cells transfected with WT or mutant human YAP1 plasmids together with vector or SIRT6 plasmids in the presence of 5 ng/ml TGF-β1. Data are presented as mean ± SEM. *p < .05, **p < .01, ***p < .001 versus vector control, and #p < .05, ##p < .001, ###p < .001 versus WT YAP.
Human Yap1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+yap1/pcDNA+Flag+Yap1+(Plasmid+%2318881)/10__1096_slash_fj__202200522r-23-0-8
Average 94 stars, based on 1 article reviews
human yap1 - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
pcdna flag yap1 y357f  (Addgene inc)
92
Addgene inc pcdna flag yap1 y357f
(A) Cre-mediated depletion of Csk in MEF cells led to the loss of pSrc Y527 inhibitory phosphorylation and a concomitant increase in pSrc Y416 activating phosphorylation without perturbing ERK phosphorylation. (B) CRISPR-Cas9 genome editing was used to mutate two tyrosine phosphorylation sites ( Yap <t>Y341F/Y357F</t> or Yap Y2F ). (C) Luminal dilation and Mist1 expression defect were not rescued in Csk CKO ; Yap Y2F mutants. (D) Yap tyrosine phosphorylation was reduced but not lost in Yap Y2F homozygous MEF cells. (E) Mutating all three tyrosine residues in a Flag-tagged Yap (Yap Y3F ) led to a complete loss of tyrosine phosphorylation as revealed by Flag or phospho-tyrosine (pY) immunoprecipitation. (F) Schematic diagram of CRISPR-Cas9 genome editing to generate Yap Y341F/Y357F/Y394F ( Yap Y3F ) and Taz Y321F ( Taz YF ) mutant mice. (G) Yap protein remained nuclear in Csk CKO ; Yap Y3F ; Taz YF mutants, resulting in luminal dilation and loss of Mist1 expression. (H) The lumen size was quantified as the ratio of the lumen area versus the overall epithelial area. One-way ANOVA, n=5, *P<0.01. (I) Quantification of Mist1+ cells. One-way ANOVA, n=5, *P<0.0001. Scale bars: 100 µm.
Pcdna Flag Yap1 Y357f, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+yap1/pcDNA+Flag+Yap1+Y357F+(Plasmid+%2318882)/bio_rxiv__2024__06__27__601065-233-10-13
Average 92 stars, based on 1 article reviews
pcdna flag yap1 y357f - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
yap tead reporter plasmid  (Addgene inc)
93
Addgene inc yap tead reporter plasmid
(A) Cre-mediated depletion of Csk in MEF cells led to the loss of pSrc Y527 inhibitory phosphorylation and a concomitant increase in pSrc Y416 activating phosphorylation without perturbing ERK phosphorylation. (B) CRISPR-Cas9 genome editing was used to mutate two tyrosine phosphorylation sites ( Yap <t>Y341F/Y357F</t> or Yap Y2F ). (C) Luminal dilation and Mist1 expression defect were not rescued in Csk CKO ; Yap Y2F mutants. (D) Yap tyrosine phosphorylation was reduced but not lost in Yap Y2F homozygous MEF cells. (E) Mutating all three tyrosine residues in a Flag-tagged Yap (Yap Y3F ) led to a complete loss of tyrosine phosphorylation as revealed by Flag or phospho-tyrosine (pY) immunoprecipitation. (F) Schematic diagram of CRISPR-Cas9 genome editing to generate Yap Y341F/Y357F/Y394F ( Yap Y3F ) and Taz Y321F ( Taz YF ) mutant mice. (G) Yap protein remained nuclear in Csk CKO ; Yap Y3F ; Taz YF mutants, resulting in luminal dilation and loss of Mist1 expression. (H) The lumen size was quantified as the ratio of the lumen area versus the overall epithelial area. One-way ANOVA, n=5, *P<0.01. (I) Quantification of Mist1+ cells. One-way ANOVA, n=5, *P<0.0001. Scale bars: 100 µm.
Yap Tead Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+yap1/pLL3%2E7+FLAG-YAP1-TEAD-P-H2B-mCherry+(Plasmid+%23128327)/pmc10489108-703-1-9
Average 93 stars, based on 1 article reviews
yap tead reporter plasmid - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
pcdna flag yap1 y357e (plasmid #18883)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
   Buy from Supplier

Image Search Results


FIGURE 9 SIRT6 deacetylates YAP1 at multiple lysine residues. (A) A diagram of human YAP1 domain structure and several known acetylated lysine residues. (B) Real-time PCR analysis of CYR61, CTGF, and ANKRD1 mRNAs in the LX-2 cells transfected with either WT or mutant human YAP1 plasmids in the presence of 5 ng/ml TGF-β1 (n = 3). (C) YAP1 acetylation analysis in LX-2 cells transfected with WT or mutant human YAP1 plasmids together with vector or SIRT6 plasmids in the presence of 5 ng/ml TGF-β1. Data are presented as mean ± SEM. *p < .05, **p < .01, ***p < .001 versus vector control, and #p < .05, ##p < .001, ###p < .001 versus WT YAP.

Journal: The FASEB Journal

Article Title: Sirtuin 6 protects against hepatic fibrogenesis by suppressing the YAP and TAZ function

doi: 10.1096/fj.202200522r

Figure Lengend Snippet: FIGURE 9 SIRT6 deacetylates YAP1 at multiple lysine residues. (A) A diagram of human YAP1 domain structure and several known acetylated lysine residues. (B) Real-time PCR analysis of CYR61, CTGF, and ANKRD1 mRNAs in the LX-2 cells transfected with either WT or mutant human YAP1 plasmids in the presence of 5 ng/ml TGF-β1 (n = 3). (C) YAP1 acetylation analysis in LX-2 cells transfected with WT or mutant human YAP1 plasmids together with vector or SIRT6 plasmids in the presence of 5 ng/ml TGF-β1. Data are presented as mean ± SEM. *p < .05, **p < .01, ***p < .001 versus vector control, and #p < .05, ##p < .001, ###p < .001 versus WT YAP.

Article Snippet: Human YAP1 was a gift from Yosef Shaul (Addgene, Cambridge, MA; plasmid # 18881; http:// n2t.net/addge ne:18881; RRID:Addgene_18881).

Techniques: Real-time Polymerase Chain Reaction, Transfection, Mutagenesis, Plasmid Preparation, Control

(A) Cre-mediated depletion of Csk in MEF cells led to the loss of pSrc Y527 inhibitory phosphorylation and a concomitant increase in pSrc Y416 activating phosphorylation without perturbing ERK phosphorylation. (B) CRISPR-Cas9 genome editing was used to mutate two tyrosine phosphorylation sites ( Yap Y341F/Y357F or Yap Y2F ). (C) Luminal dilation and Mist1 expression defect were not rescued in Csk CKO ; Yap Y2F mutants. (D) Yap tyrosine phosphorylation was reduced but not lost in Yap Y2F homozygous MEF cells. (E) Mutating all three tyrosine residues in a Flag-tagged Yap (Yap Y3F ) led to a complete loss of tyrosine phosphorylation as revealed by Flag or phospho-tyrosine (pY) immunoprecipitation. (F) Schematic diagram of CRISPR-Cas9 genome editing to generate Yap Y341F/Y357F/Y394F ( Yap Y3F ) and Taz Y321F ( Taz YF ) mutant mice. (G) Yap protein remained nuclear in Csk CKO ; Yap Y3F ; Taz YF mutants, resulting in luminal dilation and loss of Mist1 expression. (H) The lumen size was quantified as the ratio of the lumen area versus the overall epithelial area. One-way ANOVA, n=5, *P<0.01. (I) Quantification of Mist1+ cells. One-way ANOVA, n=5, *P<0.0001. Scale bars: 100 µm.

Journal: bioRxiv

Article Title: Crk mediates Csk-Hippo signaling independently of Yap tyrosine phosphorylation to induce cell extrusion

doi: 10.1101/2024.06.27.601065

Figure Lengend Snippet: (A) Cre-mediated depletion of Csk in MEF cells led to the loss of pSrc Y527 inhibitory phosphorylation and a concomitant increase in pSrc Y416 activating phosphorylation without perturbing ERK phosphorylation. (B) CRISPR-Cas9 genome editing was used to mutate two tyrosine phosphorylation sites ( Yap Y341F/Y357F or Yap Y2F ). (C) Luminal dilation and Mist1 expression defect were not rescued in Csk CKO ; Yap Y2F mutants. (D) Yap tyrosine phosphorylation was reduced but not lost in Yap Y2F homozygous MEF cells. (E) Mutating all three tyrosine residues in a Flag-tagged Yap (Yap Y3F ) led to a complete loss of tyrosine phosphorylation as revealed by Flag or phospho-tyrosine (pY) immunoprecipitation. (F) Schematic diagram of CRISPR-Cas9 genome editing to generate Yap Y341F/Y357F/Y394F ( Yap Y3F ) and Taz Y321F ( Taz YF ) mutant mice. (G) Yap protein remained nuclear in Csk CKO ; Yap Y3F ; Taz YF mutants, resulting in luminal dilation and loss of Mist1 expression. (H) The lumen size was quantified as the ratio of the lumen area versus the overall epithelial area. One-way ANOVA, n=5, *P<0.01. (I) Quantification of Mist1+ cells. One-way ANOVA, n=5, *P<0.0001. Scale bars: 100 µm.

Article Snippet: Plasmids encoding Yap 2YF and Yap 3YF were constructed from pcDNA Flag-Yap1 Y357F (Addgene #18882) through site-directed mutagenesis using Neb Q5 high-fidelity polymerase (New England Biolab #M0491).

Techniques: Phospho-proteomics, CRISPR, Expressing, Immunoprecipitation, Mutagenesis