Journal: Molecular Cancer

Article Title: CREB3L1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by remodeling the tumor microenvironment

doi: 10.1186/s12943-022-01658-x

Figure Lengend Snippet: CREB3L1 is associated with ECM signaling in thyroid cancer. A GSEA determined the enrichment differences of biological processes depending on the CREB3L1 expression. B-C The evaluated activity scores of the extracellular matrix (ECM) and collagen signaling in NT, PTC and ATC samples. D-E The Pearson correlation analysis of CREB3L1 and ECM or collagen signaling. F Immunofluorescence staining of CREB3L1 and fibroblast marker FAP in thyroid cancer samples. G RT - PCR was used to detect the expression of collagen signals after CREB3L1 knockdown. H The expression of COL5A1 was detected, after CREB3L1 knockdown or overexpression in thyroid cancer cell lines. I-J After CREB3L1 knockdown, Masson trichrome staining and IHC staining were used to detect changes in collagen fibril abundance and the expression of α - SMA after CREB3L1 knockdown, respectively. K Immunofluorescence staining of COL5A1 in ATC cell-derived xenografts after CREB3L1 knockdown. Data are shown as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 NC versus CREB3L1 - KD

Article Snippet: To analyze the α - SMA - , FAP - , or PDGFRα - positive CAFs, found in the 8505C - derived sphere or co - culture with 8505C, the samples were digested and resuspended in PBS, containing 1.5% BSA and incubated with the primary antibodies anti - α - SMA (Cat#14,395–1 - AP, Proteintech), anti - FAP (FAB3715A, R&D Systems, Minnesota, USA), and anti - PDGFRα (#567,950, BD biosciences), respectively.

Techniques: Expressing, Activity Assay, Immunofluorescence, Staining, Marker, Reverse Transcription Polymerase Chain Reaction, Knockdown, Over Expression, Immunohistochemistry, Derivative Assay

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 1. Liver-specific FGF21 knockout abrogated OVX-induced central obesity in mice. (A) Body weight profile of mice following OVX or the combination of OVX plus liver-specific FGF21 knockout. (B) The serum FGF21 concentrations in mice at decapitation. (C) Representative figures of mice indicating visceral adipose deposition at decapitation. (D) The average visceral fat weight of mice from each group. (E) Representative H&E staining of visceral adipose tissues of mice at decapitation. In figure A: * denotes p < 0.05 (OVX versus Sham/OVX+FGF21 LKO); in figure B and D: ** p < 0.01; *** p < 0.001, NS p > 0.05.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Knock-Out, Staining

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 2. Liver-specific FGF21 knockout failed to rescue OVX-induced dyslipidemia and hepatic steatosis in mice. (A) Serum concentration of triglyceride (TG). (B) Serum concentration of free fat acid (FFA). (C) Liver weight. (D) Liver TG content. (E) The representative H&E staining of liver tissues from each group. Arrows indicate lipid droplets. * p < 0.05; ** p < 0.01; *** p < 0.001; NS p > 0.05.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Knock-Out, Concentration Assay, Staining

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 3. Liver-specific FGF21 knockout exacerbated OVX-induced glucose metabolic abnormalities in mice. (A) Glucose tolerance test (GTT). (B) The area under the curve of GTT. (C) Insulin tolerance test (ITT). (D) The area under the curve of ITT. (E) Pyruvate tolerance test (PTT). (F) The area under the curve of PTT. Note figure (A,C,E): * OVX+FGF21 LKO versus Sham (p < 0.05), φ OVX+FGF21 LKO versus OVX (p < 0.05), # OVX versus Sham (p < 0.05); figure (B,D,F): * p < 0.05; ** p < 0.01; ***p < 0.001; NS p > 0.05.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Knock-Out

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 4. Transcriptomic profiling revealing Hsd11b1 plays a central role in mediating FGF21 LKO on abrogating OVX-induced central obesity in mice. (A) Venn diagram of differentially expressed genes (DEGs) among groups. (B) The number of DEGs between pairwise groups. (C) The recovered DEGs (rDEGs) in OVX mice following FGF21 LKO. rDEGs are genes whose expression in OVX was significantly different from the sham controls but recovered back to the sham levels after FGF21 LKO. (D) The heat map of rDEGs involved in lipid metabolism process (GO:0006629). Red lines indicate DEGs between OVX+FGF21 LKO versus OVX. (E) Gene set enrichment analysis (GSEA) showed FGF21 LKO reduced adipogenesis in OVX mice, and leading-edge gene analysis highlighted that Hsd11b1 plays a major role in mediating FGF21 LKO on preventing adipogenesis in OVX mice.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Expressing

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 5. FGF21 LKO reversed circulating high corticosterone but not high FSH in OVX mice. (A) Serum concentration of FSH. (B) Serum concentration of corticosterone. (C) Effects of transient recombinant FGF21 replacement on serum concentration of corticosterone in OVX+FGF21 LKO mice. (D) Effects of transient recombinant FGF21 replacement on visceral adipose Hsd11b1 expression in OVX+FGF21 LKO mice. * p < 0.05; ** p < 0.01; *** p < 0.001; NS p > 0.05.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Concentration Assay, Recombinant, Expressing

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 6. FGF21 LKO reduced serum insulin levels in OVX mice. (A) Serum concentration of insulin; (B) Gene set enrichment analysis indicated FGF21 LKO reduced SREBF-mediated lipogenesis. (C) Gene set enrichment analysis indicated FGF21 LKO even reduced insulin signaling in OVX mice compared to the sham controls. *** p < 0.001.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Concentration Assay

Journal: International journal of molecular sciences

Article Title: Hepatic-Specific FGF21 Knockout Abrogates Ovariectomy-Induced Obesity by Reversing Corticosterone Production.

doi: 10.3390/ijms241914922

Figure Lengend Snippet: Figure 7. The potential mechanism and key genes by which FGF21 LKO abrogated OVX-induced central obesity in mice. (A) The potential mechanism by which FGF21 LKO abrogated OVX-induced central obesity in mice. Liver-specific FGF21 knockout reduced both GC and insulin production, which in turn decreased adipogenesis and lipogenesis in visceral adipose tissues. (B) The potential key genes mediating FGF21 LKO on abrogating OVX-induced central obesity in mice.

Article Snippet: Four weeks after surgery procedures (i.e., at 10 week of age), a subgroup of OVX+FGF21 LKO mice were injected via tail vein with recombinant mouse FGF21 (RD272108100, BioVendor, Prague, Czech Republic) at a dose of 1 mg/kg body weight at 0800 AM, according to our previous descriptions [29].

Techniques: Knock-Out

Journal: bioRxiv

Article Title: Mechanical, Biochemical, and Multicellular Effects on Vessel Network Morphometrics in a Microfluidic Vasculature-on-a-Chip

doi: 10.1101/2025.08.28.672831

Figure Lengend Snippet: a) Fluorescence images showing vessel network development over 7 days (images shown are for FB1:EC5). Red: RFP-HUVECs; green: GFP-normal human lung fibroblasts. b) Illustration of fluorescent microbeads perfused through MVN and sample fluorescence image of microbeads in MVN. c) Overlay fluorescence image of microbeads (blue) in the vessel network (red), with two insets. (i) Magnification of inset showing a vessel branch with no beads, suggesting perfusion did not reach that branch (white arrows). (ii) Magnification of inset with all vessel branches perfused by beads. Scale bar = 500 mm for a) to c). d) Beads leaked out of vessels frequently near branches with jagged edges. e) Vessel branches with smooth edges contained the beads without leakage. f) Localized vessel leak near a vessel opening. (Top: raw image of vessels; Bottom: red = vessels, green = beads).

Article Snippet: Red fluorescent protein-expressing human umbilical vein endothelial cells (RFP-HUVECs, cAP-0001RFP) and green fluorescent protein-expressing human adult lung fibroblasts (GFP-hLFs, cAP-0033GFP) were purchased from Angio-Proteomie (Boston, MA, USA) and cultured on T75 flasks coated with Quick Coating solution (cAP-01, Angio-Proteomie) according to manufacturer’s protocol.

Techniques: Fluorescence

Journal: bioRxiv

Article Title: Mechanical, Biochemical, and Multicellular Effects on Vessel Network Morphometrics in a Microfluidic Vasculature-on-a-Chip

doi: 10.1101/2025.08.28.672831

Figure Lengend Snippet: Morphometric analysis of fibroblast effects on MVN morphology: branched-based metrics. a) Fluorescence images of RFP-HUVECs forming MVNs in the middle channel. Scale bar = 500 mm. b) Line plots of average percent vessel area over time. Shaded regions represent standard deviations. c) Bar graph of average percent vessel area grouped by days in culture. Each data point represents percent vessel area of an individual device unit (n=10, 23, 26, 16, and 12 for EGM-2, FB1:EC5, FB5:EC5, FB-CM, and Ang-V50, respectively). d) Boxplot of individual vessel branch diameters (measurable, as defined in ) on Day 7. Red line is the median; box ranges from 25 th to 75 th percentiles. Each data point represents a single vessel diameter, collected from all the device units for each condition. e) Bar graph of number of diameter outliers (as defined in ) per device unit. For d) and e), n = 10, 23, 32, 16, and 12 for EGM-2, FB1:EC5, FB5:EC5, FB-CM, and Ang-V50, respectively. A few vessel units included in d) and e) were excluded from b) and c) because the prerequisite binary conversion using a global threshold failed to capture the true morphology of the vessel network in these units. For all graphs: *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: Red fluorescent protein-expressing human umbilical vein endothelial cells (RFP-HUVECs, cAP-0001RFP) and green fluorescent protein-expressing human adult lung fibroblasts (GFP-hLFs, cAP-0033GFP) were purchased from Angio-Proteomie (Boston, MA, USA) and cultured on T75 flasks coated with Quick Coating solution (cAP-01, Angio-Proteomie) according to manufacturer’s protocol.

Techniques: Fluorescence

Journal: bioRxiv

Article Title: Mechanical, Biochemical, and Multicellular Effects on Vessel Network Morphometrics in a Microfluidic Vasculature-on-a-Chip

doi: 10.1101/2025.08.28.672831

Figure Lengend Snippet: Morphometric analysis of fibroblast effects on MVN morphology: void-based metrics. a) Temporal line plots and d) bar graph (grouped by days in culture) of void count per device unit. b) Temporal line plots and e) box plot (grouped by days in culture) of average void area per device unit. c) Temporal line plots and f) box plot (grouped by days in culture) of average void roundness per device unit. Shaded regions represent standard deviations. Each data point represents either total void count in a device unit for d), or the average void area or void roundness across all voids in a device unit for e) and f). g) Polar histograms of void angles over time. Angles measured from the horizontal x-axis as defined in . Angles between 90 to 180 degrees were converted to their supplementary angles. For a) to g): n = 10, 23, 26, 16, and 12 for EGM-2, FB1:EC5, FB5:EC5, FB-CM, and Ang-V50, respectively. h) Bar graph of percent perfusable vessel networks, calculated as number of perfusable MVN units divided by total tested MVN device units. Blue: perfusable units with leakage Red: perfusable units without leakage. For h): n = 10,18,29,12, and 12 for EGM-2, FB1:EC5, FB5:EC5, FB-CM, and Ang-V50, respectively. *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: Red fluorescent protein-expressing human umbilical vein endothelial cells (RFP-HUVECs, cAP-0001RFP) and green fluorescent protein-expressing human adult lung fibroblasts (GFP-hLFs, cAP-0033GFP) were purchased from Angio-Proteomie (Boston, MA, USA) and cultured on T75 flasks coated with Quick Coating solution (cAP-01, Angio-Proteomie) according to manufacturer’s protocol.

Techniques: