Journal: Journal of the Royal Society Interface

Article Title: Macro-to-nanoscale investigation of wall-plate joints in the acorn barnacle Semibalanus balanoides : correlative imaging, biological form and function, and bioinspiration

doi: 10.1098/rsif.2019.0218

Figure Lengend Snippet: Schematic of the multimodal and multiscale correlative workflow using LM, XRM, SEM and FIB-SEM. These techniques can be correlated using Zeiss Microscopy Atlas 5 (3D) software. (Online version in colour.)

Article Snippet: Stages of the FIB-SEM automated milling process using Zeiss Microscopy Atlas 5 (3D). ( a ) An overlay is created for each part of the milling preparation. ( b ) Once a platinum pad has been deposited over an initial platinum pad and the milled reference marks creating a ‘sandwich’, a trench is milled to reveal a cross-sectional face ( c ). ( d ) Acquired images can then be stacked together to create a tomographic volume. (Online version in colour.)

Techniques: Microscopy, Software

Journal: Journal of the Royal Society Interface

Article Title: Macro-to-nanoscale investigation of wall-plate joints in the acorn barnacle Semibalanus balanoides : correlative imaging, biological form and function, and bioinspiration

doi: 10.1098/rsif.2019.0218

Figure Lengend Snippet: Stages of the FIB-SEM automated milling process using Zeiss Microscopy Atlas 5 (3D). ( a ) An overlay is created for each part of the milling preparation. ( b ) Once a platinum pad has been deposited over an initial platinum pad and the milled reference marks creating a ‘sandwich’, a trench is milled to reveal a cross-sectional face ( c ). ( d ) Acquired images can then be stacked together to create a tomographic volume. (Online version in colour.)

Article Snippet: Stages of the FIB-SEM automated milling process using Zeiss Microscopy Atlas 5 (3D). ( a ) An overlay is created for each part of the milling preparation. ( b ) Once a platinum pad has been deposited over an initial platinum pad and the milled reference marks creating a ‘sandwich’, a trench is milled to reveal a cross-sectional face ( c ). ( d ) Acquired images can then be stacked together to create a tomographic volume. (Online version in colour.)

Techniques: Microscopy

Journal: International Journal of Nanomedicine

Article Title: Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium

doi: 10.2147/IJN.S92733

Figure Lengend Snippet: SEM morphologies of various substrates. Notes: SEM images of Ti samples at low and high magnifications with various cathodic arc discharge plasma treatment times of 1 second ( B , b1, and b2), and 2 seconds ( C , c1, and c2) when compared to untreated Ti control ( A , a1, and a2). EDS pictures of elements on (a3) uncoated, (b3) 1 second nanocoated, and (c3) 2 seconds coated samples. Abbreviations: SEM, scanning electron microscopy; EDS, energy-dispersive X-ray spectrometer.

Article Snippet: A scanning electron microscopy (SEM, Zeiss NVision 40 FIB) equipped with an energy-dispersive X-ray spectrometer (EDS) was employed to qualitatively assess the surface morphologies and elements after nanocoating.

Techniques: Clinical Proteomics, Control, Electron Microscopy

Journal: International Journal of Nanomedicine

Article Title: Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium

doi: 10.2147/IJN.S92733

Figure Lengend Snippet: AFM images of samples with 0 seconds ( A ), 1 second ( B ), and 2 seconds ( C ) plasma coating. Abbreviation: AFM, atomic force microscopy.

Article Snippet: A scanning electron microscopy (SEM, Zeiss NVision 40 FIB) equipped with an energy-dispersive X-ray spectrometer (EDS) was employed to qualitatively assess the surface morphologies and elements after nanocoating.

Techniques: Clinical Proteomics, Microscopy

Journal: International Journal of Nanomedicine

Article Title: Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium

doi: 10.2147/IJN.S92733

Figure Lengend Snippet: MSC adhesion on various substrates. Notes: ( A ) Enhanced MSC adhesion on plasma-coated Ti after 4 hours of culture. Data are mean ± standard error of the mean, N=3. * P <0.01 and # P <0.01 when compared to respective Ti controls and glass references, ** P <0.01 when compared to all other Ti samples, and ## P <0.01 when compared to 1 second plasma-coated glass. ( B ) Fluorescence microscopy images of DAPI-stained MSC attachment density on various substrates (10× magnification). Abbreviations: MSC, mesenchymal stem cell; DAPI, 4′,6-diamidino-2-phenylindole dihydrochloride.

Article Snippet: A scanning electron microscopy (SEM, Zeiss NVision 40 FIB) equipped with an energy-dispersive X-ray spectrometer (EDS) was employed to qualitatively assess the surface morphologies and elements after nanocoating.

Techniques: Clinical Proteomics, Fluorescence, Microscopy, Staining

Journal: International Journal of Nanomedicine

Article Title: Enhanced human bone marrow mesenchymal stem cell functions on cathodic arc plasma-treated titanium

doi: 10.2147/IJN.S92733

Figure Lengend Snippet: Typical MSC-spreading morphologies (confocal microscopy images, 20× magnification, images are ~1 mm 2 ) after 1 day, 3 days, and 5 days proliferation on: the Ti control, the 1 second plasma-coated Ti, and the 2 seconds plasma-coated Ti samples. Notes: Blue color represents nuclei stained by DAPI; red color represents cytoskeleton stained by Rhodamine-Phalloidin. Abbreviations: MSC, mesenchymal stem cell; DAPI, 4′,6-diamidino-2-phenylindole dihydrochloride.

Article Snippet: A scanning electron microscopy (SEM, Zeiss NVision 40 FIB) equipped with an energy-dispersive X-ray spectrometer (EDS) was employed to qualitatively assess the surface morphologies and elements after nanocoating.

Techniques: Confocal Microscopy, Control, Clinical Proteomics, Staining