Journal: Nature Methods

Article Title: A polarized FGF8 source specifies frontotemporal signatures in spatially oriented cell populations of cortical assembloids

doi: 10.1038/s41592-024-02412-5

Figure Lengend Snippet: a , Schematic summarizing factors and gene markers involved in human cortical area specification. b , Overview of the protocol used to treat neural organoids with patterning factors from 3 to 5 div. Diff, differentiation medium ± vitamin A. c , Percentage of organoids with detectable SP8 > GFP expression after treatment with rostralizing factors (left) or EMX1>mNeonGreen expression after treatment with caudalizing factors (right). CHRDL1, chordin like 1; FST, follistatin; SB, SB-431542 dual SMAD inhibitor; CER1, cerberus 1; CHIR, GSK-3β inhibitor CHIR99021; WNT1, Wnt family member 1. Data indicate the mean ± s.d. from three lines per treatment ( n = 15 organoids per condition and line; except n = 16 for FGF8 high and CHIR low; n = 20, n = 19 and n = 17 for Chir high, and n = 18, n = 19 and n = 17 for Wnt1 treatment for the three lines). P values from one-way analysis of variance (ANOVA; Tukey’s multiple-comparisons test) for comparisons to untreated conditions are provided. d , Experimental procedure for assembloid generation with the SP8 > GFP line and OrEBs. D, day after EB formation; Diff, differentiation medium ± vitamin A; ULA, ultra-low attachment plate . e , RT–qPCR analysis of FGF8 target genes expressed in the SP8 > GFP EBs severed from OrEB after 1 day of co-culture. Data are the log of expression over TBP , shown as the mean ± s.d. ( n = 6 EBs for 0%, n = 5 for 1%, n = 5 for 10% grown from three independent clones). P values are the results of one-way ANOVA. f , GFP intensity of the organoids from g . Whiskers are the minima to maxima, boxes represent the 25th to 75th percentiles (Q1 to Q3) and lines indicate the median ( n = 7 organoids for 0%, n = 11 for 1%, n = 19 for 10%, grown from three independent clones). P values are the results of one-way ANOVA. g , Images of organoids generated with the SP8 > GFP transgenic line and co-culture with OrEBs at 60 div (scale bars, 500 µm). **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.1. a.u., arbitrary units.

Article Snippet: Levels of FGF8 protein in cell extracts and supernatants were measured using an ELISA kit (Cusabio CSB-E15861h) according to the manufacturer’s instruction.

Techniques: Expressing, Quantitative RT-PCR, Co-Culture Assay, Clone Assay, Generated, Transgenic Assay

Journal: Nature Methods

Article Title: A polarized FGF8 source specifies frontotemporal signatures in spatially oriented cell populations of cortical assembloids

doi: 10.1038/s41592-024-02412-5

Figure Lengend Snippet: a ) Schematic diagram of CAG > FGF8 reporter (top). Genotyping of the selected clones (bottom) shows the three heterozygotes selected. The PCR was designed to amplify a wild type AAVS1 (WT) amplicon, a left homology arm amplicon (LA), a right homology arm amplicon (RA), and an inner part (IP). b ) RT-qPCR analysis of pluripotency markers and differentiation gene T/BRA in the selected CAG > FGF8 expressing clones and parental wild-type cells. Values are mean ± SD (n = 4 biological replicates for wt; n = 3 biological replicates, one for each CAG > FGF8 clone). P-values resulting from one-way ANOVA (Tukey’s multiple comparisons test) among the different lines are: OCT4 wt vs. OCT4 CAG > FGF8, p = 0.9166; NANOG wt vs. NANOG CAG > FGF8, p = 0.9841; SOX2 wt vs. SOX2 CAG > FGF8, p = 0.9997; TBRA wt vs. TBRA CAG > FGF8, p > 0.9999. c ) Box plots showing RT-qPCR analysis of FGF8 and WNT1 genes in the selected clones of the CAG > FGF8 line compared to wt. Whiskers are min to max, boxes represent the 25th to 75th percentiles (Q1 to Q3); n = 6 from 3 independent clones. One-way ANOVA analysis is p < 0.0001 for FGF8 expression and p > 0.9999 for WNT1 expression. d ) Quantification of FGF8 protein levels in cell lysates (CELLS) and supernatants (SUP) measured by ELISA assay. Values are mean ± SD, n = 6 from 3 independent clones; P-values from two-sided unpaired t-tests are: CELLS wt vs CELLS CAG > FGF8, p = 0.0004; SUP wt vs SUP CAG > FGF8, p = 0.0823.

Article Snippet: Levels of FGF8 protein in cell extracts and supernatants were measured using an ELISA kit (Cusabio CSB-E15861h) according to the manufacturer’s instruction.

Techniques: Clone Assay, Amplification, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Nature Methods

Article Title: A polarized FGF8 source specifies frontotemporal signatures in spatially oriented cell populations of cortical assembloids

doi: 10.1038/s41592-024-02412-5

Figure Lengend Snippet: a ) Representative images of 4 div embryoid bodies co-cultured with OrEBs containing cells expressing FGF8 and tdTomato mixed at different percentage (0%, 1%, 10%), immunostained for the FGF8 downstream target ETV1 (green) (scale bars 50 µm, the SP8 > GFP organoid is outlined with a dashed contour). The experiment has been repeated 4 times with organoids derived from three independent clones. b ) Immunostaining of 60 div organoids generated with the SP8 > GFP transgenic line and co-cultured with OrEBs in Fig. , stained for the rostral marker LMO4 (scale bars 500 µm in the main panels, 50 µm for the insets). The SP8 > GFP organoid is outlined with a dashed contour. c ) Box plots showing the fraction of LMO4+ cells normalized to total DAPI+ cells in 0%, 1% and 10% FGF8 conditions at 60 div. Whiskers are min to max, boxes represent the 25th to 75th percentiles (Q1 to Q3); n = 8 organoids. P-values from one-way ANOVA (Tukey’s multiple comparisons tests) are: 0% vs 1%, p = 0.0019, 0% vs 10%, p < 0.0001, 1% vs 10%, p < 0.0001. d ) Immunostaining of organoids generated with the SP8 > GFP transgenic line and co-cultured with OrEBs as described in Fig. for 60 days, stained for the neural marker Sox1 and DAPI. Scale bars 500 µm. e ) Quantification of the number of Sox1+ cells over total (DAPI + ) cells. Data show mean ± SD (n = 2 assembloids for 0%, n = 4 assembloids for 1%, n = 3 assembloids for 10%). P-values for each comparison resulting from one-way ANOVA (Tukey’s multiple comparisons tests) are: 0% vs 1%, p = 0.113; 0% vs 10%, p = 0.2171; 1% vs 10%, p = 0.8893. f ) RT-qPCR analysis of anterior ( PAX6, SIX3 ) and optic cup ( SNAI2, PAX3, OTX2, LHX2, RAX ) markers from SP8 > GFP organoids severed from the co-culture experiment at 60 div. Data is Log of expression over TBP, shown as mean ± SD; n = 6 for 0% condition, n = 5 for 1% and 10% condition, from 3 independent clones. P-values from one-way ANOVA (Tukey’s multiple comparisons tests) are: RAX 0% vs. RAX 1%, p = 0.0718; RAX 0% vs. RAX10%, p = 0.0003; RAX 1% vs. RAX 10%, p = 0.0263; all the other comparisons are p < 0.0001. g ) GFP intensity per segment (P, proximal; M, medial; D, distal) in the 1% FGF8 condition from experiments shown in panel b . Each segmented line represents an individual organoid. n = 7 from 3 clones; ns, p = 0.5783 one-way ANOVA. Ns, non-significant. a.u., arbitrary units.

Article Snippet: Levels of FGF8 protein in cell extracts and supernatants were measured using an ELISA kit (Cusabio CSB-E15861h) according to the manufacturer’s instruction.

Techniques: Cell Culture, Expressing, Derivative Assay, Clone Assay, Immunostaining, Generated, Transgenic Assay, Staining, Marker, Comparison, Quantitative RT-PCR, Co-Culture Assay

Journal: Nature Methods

Article Title: A polarized FGF8 source specifies frontotemporal signatures in spatially oriented cell populations of cortical assembloids

doi: 10.1038/s41592-024-02412-5

Figure Lengend Snippet: a , Experimental procedure for elongated assembloids using mosaic OrEBs containing CAG>tdTOMATO and non-fluorescent CAG > FGF8 -expressing cells. Diff, differentiation medium ± vitamin A. b , Representative images of elongated cortical assembloids at 1 div in the PDMS molds (indicated by white arrows in (i); scale bar, 500 µm), at 7 div after removal from the molds and before Matrigel embedding (ii) or after embedding in large Matrigel droplets ((iii); scale bar, 5 mm), at 120 div in the six-well plate ((iv); scale bar, 5 mm). c , Position of the OrEB on elongated cortical assembloids length (as a percentage) at 15, 60 and 120 div. Values are the mean ± s.d. ( n = 9 organoids for 15 div, n = 11 for 60 div and 120 div grown from three independent clones). P values for comparisons among time points (one-way ANOVA Tukey’s multiple-comparisons test) are: 15 div versus 60 div, P = 0.9455; 15 div versus 120 div, P = 0.9781; 60 div versus 120 div, P > 0.9999. d , Images of elongated cortical assembloids generated with the SP8 > GFP transgenic line and mosaic OrEBs at 60 div (scale bars, 500 µm). Right, SP8 > GFP intensity per segment (P, M and D) in individual assembloids. Each segmented line represents an individual elongated cortical assembloid ( n > 2 from at least 2 clones). P values are the results of one-way ANOVA among segments per condition (0%, P = 0.9045; 1%, P < 0.0001 and 10%, P = 0.9828). e , f , Images of proximal and distal CPNE8 ( e ) or NR2F1 ( f ) stainings (scale bars, 50 µm). Bottom, fraction of CPNE8 + ( e ) or NR2F1 + cells ( f ) normalized to total (DAPI + ) cells in proximal and distal insets of controls (conCAs) and polCAs at 60 div. Whiskers are min to max, boxes represent the 25th to 75th percentiles (Q1 to Q3) and lines indicate the median; CPNE8: n = 20 insets for P and D from 3 conCAs, n = 42 insets for P and n = 39 for D from 3 polCAs; NR2F1: n = 60 insets for P and D from 6 conCAs, n = 40 insets for P and D from 4 polCAs. P values from one-way ANOVA (Tukey’s multiple-comparisons test) are: P = 0.9988 in CPNE8 proximal conCA versus distal conCAs, P = 0.8509 in NR2F1 proximal conCAs versus distal conCAs, P < 0.0001 for other comparisons; NS, not significant. g , g ′, Images of 60 div polCA immunostained with tdTomato in red, DAPI in blue and NR2F1 in white ( g ) or intensity rainbow ( g ′). Scale bar, 500 µm.

Article Snippet: Levels of FGF8 protein in cell extracts and supernatants were measured using an ELISA kit (Cusabio CSB-E15861h) according to the manufacturer’s instruction.

Techniques: Expressing, Clone Assay, Generated, Transgenic Assay

Journal: Nature Methods

Article Title: A polarized FGF8 source specifies frontotemporal signatures in spatially oriented cell populations of cortical assembloids

doi: 10.1038/s41592-024-02412-5

Figure Lengend Snippet: a-b ) Representative images of elongated assembloids (8 div) properly embedded maintaining elongated shape (a) compared to elongated assembloids from the same batch without matrigel, shrinking without spatial constrain (b). c ) Length of elongated assembloids after different days in vitro (div, div1 n = 8, div7 n = 6, div30 n = 4, div60 n = 24, div120 n = 22 assembloids grown from 3 independent clones). P-values resulting from one-way ANOVA Tukey’s multiple comparisons tests) among the different lines are: div1 vs div7, p = 0.0045; div1 vs div30, p = 0.9999; div7 vs div30, p = 0.0366; div7 vs div60, p = 0.9998; div7 vs div120, p = 0.51; div30 vs div60, p = 0.0049; div60 vs div120, p = 0.1825; div1 vs div60, div1 vs div120, div30 vs div120, p < 0.0001. d ) RT-qPCR analysis of neural markers (SOX2, SOX1, SP8 and LMO4), ventral marker NXK2.1 and differentiation markers SNAI1 and T-BRA, in elongated assembloids (eOrg) compared to round organoids (rOrg), after treatment with high FGF8 as described in Fig. . Values are mean ± SD, n = 3 in three lines (hESC H9, H1 and iPSC 178/5). P-values resulting from one-way ANOVA (Tukey’s multiple comparisons tests) are: eORGSOX2 d10 vs. rORGSOX2 d10, p = 0.0024; eORGSOX2 d30 vs. rORGSOX2 d30, p = 0.3144; eORGSOX2 d60 vs. rORGSOX2 d60, p = 0.9434; eORGSOX1 d10 vs. rORGSOX1 d10, p = 0.0009; eORGSOX1 d30 vs. rORGSOX1 d30, p = 0.1573; eORGSOX1 d60 vs. rORGSOX1 d60, p = 0.7266; eORGSP8 d10 vs. rORGSP8 d10, p = 0.0006; eORGSP8 d30 vs. rORGSP8 d30, p = 0.1891; eORGSP8 d60 vs. rORGSP8 d60, p = 0.8004; eORGLMO4 d10 vs. rORGLMO4 d10, p = 0.0114; eORGLMO4 d30 vs. rORGLMO4 d30, p = 0.8378; eORGLMO4 d60 vs. rORGLMO4 d60, p = 0.9821; eORGNKX2.1 d10 vs. rORGNKX2.1 d10, p = 0.0008; eORGNKX2.1 d30 vs. rORGNKX2.1 d30, p = 0.1345; eORGNKX2.1 d60 vs. rORGNKX2.1 d60, p = 0.9036; eORGSNAI1 d10 vs. rORGSNAI1 d10, p = 0.0026; eORGSNAI1 d30 vs. rORGSNAI1 d30, p = 0.6125; eORGSNAI1 d60 vs. rORGSNAI1 d60, p = 0.96; eORGT-BRA d10 vs. rORGT-BRA d10, p = 0.6815; eORGT-BRA d30 vs. rORGT-BRA d30, p = 0.3026; eORGT-BRA d60 vs. rORGT-BRA d60, p = 0.1297. e ) Fraction of TUNEL+ cells normalized to total DAPI+ cells in elongated versus conventional round organoids (div, days in vitro ). N = 36 insets for 4 div elongated, n = 33 for 4 div Round, n = 122 for 7 div elongated, n = 37 for 7 div Round, n = 81 for 30 div elongated, n = 82 for 30 div Round organoids from 3 lines per condition. P-values resulting from one-way ANOVA (Tukey’s multiple comparisons tests) are: 4 div eOrg vs. 4 div rOrg, p < 0.0001; 7 div eOrg vs. 7 div rOrg, p = 0.31; 30 div eOrg vs. 30 div rOrg, p = 0.0115. f ) Box plots showing the fraction of TUNEL+ cells normalized to total DAPI+ cells in proximal and distal insets of elongated assembloids (n = 18 insets for 4 div Proximal and Distal, n = 32 for 7 div Proximal, n = 33 for 7 div Distal, n = 40 for 30 div Proximal, n = 42 for 30 div Distal; organoids are grown from 3 lines per condition). P-values resulting from one-way ANOVA (Tukey’s multiple comparisons tests) are: 4 div proximal vs. 4 div distal, p = 0.6097; 7 div proximal vs. 7 div distal, p = 0.6559; 30 div proximal vs. 30 div distal, p = 0.9897. g ) Representative images of the OrEB localization throughout elongated assembloids growth quantified in Fig. (scale bars 500 µm). h ) Top, representative images of proximal and distal CTIP2 staining (scale bars 50 µm). Bottom, fraction of CTIP2+ cells normalized to total DAPI+ cells in proximal and distal insets of controls (conCA) and polCAs at 60 div (n = 50 insets for P and D conCAs, n = 40 insets for P and D polCAs from 3 organoids). P-values resulting from one-way ANOVA (Tukey’s multiple comparisons tests) are: proximal conCA vs. distal conCA, p = 0.8204; proximal polCA vs. distal polCA, p < 0.0001. i ) Top, representative images of proximal and distal LMO4 staining (scale bars 50 µm). Bottom, fraction of LMO4+ cells normalized to total DAPI+ cells in proximal and distal insets of control(tdt) and polCAs at 60 div (n = 61 insets for P and D from 6 conCAs, n = 51 insets for P and D from 4 polCA). P-values resulting from one-way ANOVA (Tukey’s multiple comparisons tests) are: proximal conCA vs. distal conCA, p > 0.9999; proximal polCA vs. distal polCA, p < 0.0001. ns, non-significant. All whiskers are min to max, boxes represent the 25th to 75th percentiles (Q1 to Q3) and lines indicate median. j-j’ ) Images of immunostained longitudinal sections of elongated assembloids at 60 div (scale bars 500 µm).

Article Snippet: Levels of FGF8 protein in cell extracts and supernatants were measured using an ELISA kit (Cusabio CSB-E15861h) according to the manufacturer’s instruction.

Techniques: In Vitro, Clone Assay, Quantitative RT-PCR, Marker, TUNEL Assay, Staining, Control

Journal: Nature Methods

Article Title: A polarized FGF8 source specifies frontotemporal signatures in spatially oriented cell populations of cortical assembloids

doi: 10.1038/s41592-024-02412-5

Figure Lengend Snippet: a ) UMAP embedding for the scRNA-seq dataset containing cells derived from dissections of three segments of polCA and control counterparts annotated by stress levels based on Gruffi analysis. b ) Sankey plot showing similar mapping of cells from individual segments of both control and polCA to stressed cells according to Gruffi’s results. c ) Box plots showing the number of cells expressing FGF8 normalized by the total number of cells per segment for the three replicates. The box displays the median, the inter-quartile range, the minimum and maximum values for each segment. d ) Dot plot for the top five markers for each cluster, genes with logFC> 3 are shown. LogFCs colors are scaled by gene. e ) UMAP embedding plots colored by expression levels of markers of radial glia ( NES, SOX2, VIM ), cycling radial glia ( TOP2A, MKI67 ), Cajal-Retzious cells ( RELN ), neurons ( DCX, TUBB3 ), interneuron progenitor cells ( DLX6-AS1, GAD2 ), LGE-derived progenitors ( GSX2 ), MGE-derived progenitors ( NKX2.1 ), retinal progenitor cells ( RORB, VSX2 ), stress responsive cells ( GOLGA4 ), endothelial cells ( DCN, BGN, COL1A2 ), BMP responsive cells ( TTR, RSPO2, LMX1A, MSX1 ), cilium bearing cells ( PCP4, NPHP1 ), oligodendrocytes ( OLIG1, OLIG2 ), astrocytes ( GFAP, AQP4 ) and microglia ( AIF1, CD68 ).

Article Snippet: Levels of FGF8 protein in cell extracts and supernatants were measured using an ELISA kit (Cusabio CSB-E15861h) according to the manufacturer’s instruction.

Techniques: Derivative Assay, Control, Expressing

Journal: The FASEB Journal

Article Title: Loss of the RNA Binding Protein HuR in Early Murine Limb Mesenchyme Does Not Affect Development but Leads to Impaired Bone Homeostasis in Adulthood

doi: 10.1096/fj.202500780RR

Figure Lengend Snippet: Effect of germline Cre recombination in HuR fl/fl mice (Elavl1 KO) through maternal inheritance of Prx1‐Cre. (A) Whole‐mount embryos at E13.5 stained with alcian blue. (B) Toluidine blue‐stained sections of E13.5 embryo forelimbs and hindlimbs. (C) Immunofluorescence detection of HuR in limb sections from E13.5 embryos. (D) Brightfield micrographs demonstrating morphology changes in Control and Elavl1 KO embryos isolated from E9.5 to E11.5. Scale bar = 500 μm. (E) Whole‐mount in situ hybridization chain reaction detection of limb bud markers mRNA in E10.5 and E11.5 embryos. Asterisk: Limb bud, Triangle: Apical ectodermal ridge, Arrowhead: The zone of polarizing activity. Scale bar = 150 μm. (F) Whole‐mount immunofluorescence detection of Fgf8 protein in E10.5 embryos. Asterisk: Limb bud, Triangle: Apical ectodermal ridge. Scale bar = 150 μm.

Article Snippet: Embryos were fixed in 4% formaldehyde for 1 h at 4°C, washed, and stored in 0.0025% Triton X‐100 in PBS (PBS‐Tr) at 4° C. Prior to immunofluorescence staining, embryos were permeabilized with 0.25% Triton X‐100 in PBS for 30 min and then blocked with 2% bovine serum albumin in PBS solution for 1 h before incubation with 1:100 Fgf8 antibody (MAB323, R&D Systems, UK) overnight at 4°C.

Techniques: Staining, Immunofluorescence, Control, Isolation, In Situ Hybridization, Activity Assay

Journal: Oncogene

Article Title: Retinoid-suppressed phosphorylation of RARalpha mediates the differentiation pathway of osteosarcoma cells.

doi: 10.1038/onc.2010.50

Figure Lengend Snippet: Figure 7 RARa hypophosphorylation induces FGF8f expression. (a) Primers designed for analysis of total FGF8 and FGF8 isoforms (the diagram does not reflect the actual dimension flanked by the primers). (b, c) RT–PCR analysis of FGF8 isoforms and FGF8f expression. (d, e) WB analysis of FGF8f proteins in the lysates (panel d) and in the medium of U2OS cells expressing RARaS77A (panel e).

Article Snippet: Lentiviral plasmid construction, virion production and transduction The RARaS77A cDNA (Luo et al., 2007) and FGF8f cDNA (OriGene, Rockville, MD, USA) were cloned into pCCL-cMNDU3c-X2-PKG-EGFP lentiviral vector (Luo et al., 2007).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Oncogene

Article Title: Retinoid-suppressed phosphorylation of RARalpha mediates the differentiation pathway of osteosarcoma cells.

doi: 10.1038/onc.2010.50

Figure Lengend Snippet: Figure 8 FGF8f is a downstream target of RARa hypophosphorylation in RA-induced osteoblastic differentiation. (a) Morphology of U2OS cells transduced with FGF8f. (b) Proliferation analysis by cell count. (c, d) RT–PCR depicted expression of differentiation regulators. (e, f) WB analyses of FGF8f and OPN proteins. (g) FGF8-siRNA decreased FGF8f mRNA levels. B-peptide, blocking peptide; C-siRNA, control siRNA. (h) FGF8-siRNA decreased levels of OPN mRNA. (i) FGF8-siRNA counteracted the effects of either RA or FGF8f on inhibition of proliferation. siRNA vs vehicle: *P ¼ 0.000; **Po0.005. (j) FGF8 antibodies neutralized the inhibition effect of FGF8f on cell proliferation. FGF8 antibodies vs PBS: #Po0.02; *Po0.04; **Po0.003.

Article Snippet: Lentiviral plasmid construction, virion production and transduction The RARaS77A cDNA (Luo et al., 2007) and FGF8f cDNA (OriGene, Rockville, MD, USA) were cloned into pCCL-cMNDU3c-X2-PKG-EGFP lentiviral vector (Luo et al., 2007).

Techniques: Transduction, Cell Counting, Reverse Transcription Polymerase Chain Reaction, Expressing, Blocking Assay, Control, Inhibition

Journal: Oncogene

Article Title: Retinoid-suppressed phosphorylation of RARalpha mediates the differentiation pathway of osteosarcoma cells.

doi: 10.1038/onc.2010.50

Figure Lengend Snippet: Figure 9 Hypothetical network of differentiation response path- ways coordinated by RA-RARa-FGF8f signal transduction during osteoblastic differentiation.

Article Snippet: Lentiviral plasmid construction, virion production and transduction The RARaS77A cDNA (Luo et al., 2007) and FGF8f cDNA (OriGene, Rockville, MD, USA) were cloned into pCCL-cMNDU3c-X2-PKG-EGFP lentiviral vector (Luo et al., 2007).

Techniques: Transduction

Journal: Journal of Translational Medicine

Article Title: Mutations in fibroblast growth factor (FGF8) and FGF10 identified in patients with conotruncal defects

doi: 10.1186/s12967-020-02445-2

Figure Lengend Snippet: Characteristics of missense variants identified in FGF8 and FGF10

Article Snippet: The human FGF8 (pCMV6-Entry-FGF8, Myc-DDK-tagged) and FGF10 (pCMV6-Entry-FGF10, Myc-DDK-tagged) cDNA plasmids were purchased from Origene (Rockville, MD, USA).

Techniques: Biomarker Discovery, Mutagenesis

Journal: Journal of Translational Medicine

Article Title: Mutations in fibroblast growth factor (FGF8) and FGF10 identified in patients with conotruncal defects

doi: 10.1186/s12967-020-02445-2

Figure Lengend Snippet: Distribution and conservation of mutations in FGF8 and FGF10. a , b Alignment of multiple FGF8 and FGF10 protein sequences among species. c , d Structure of the FGF8 and FGF10 proteins and the location of the genetic variations in this study

Article Snippet: The human FGF8 (pCMV6-Entry-FGF8, Myc-DDK-tagged) and FGF10 (pCMV6-Entry-FGF10, Myc-DDK-tagged) cDNA plasmids were purchased from Origene (Rockville, MD, USA).

Techniques:

Journal: Journal of Translational Medicine

Article Title: Mutations in fibroblast growth factor (FGF8) and FGF10 identified in patients with conotruncal defects

doi: 10.1186/s12967-020-02445-2

Figure Lengend Snippet: The expression and intracellular localization of wild-type and mutated FGF8. Relative mRNA expression of wild-type and variants of FGF8 in HCM ( a ) and HEK 293T ( c ), respectively (n = 3). GAPDH was used as an internal control. Western blot analysis and density quantitation in HCM ( b ) and HEK 293T ( d ) transfected with the blank vector, wild-type, and mutated FGF8. GAPDH and β-actin were used as an internal control in HCM and HEK293T, respectively (n = 3). e Immunofluorescence staining of wild-type and variants of FGF8. Images represented here were obtained from 3 biological replicates. Scale bar 20 μm

Article Snippet: The human FGF8 (pCMV6-Entry-FGF8, Myc-DDK-tagged) and FGF10 (pCMV6-Entry-FGF10, Myc-DDK-tagged) cDNA plasmids were purchased from Origene (Rockville, MD, USA).

Techniques: Expressing, Control, Western Blot, Quantitation Assay, Transfection, Plasmid Preparation, Immunofluorescence, Staining

Journal: Journal of Translational Medicine

Article Title: Mutations in fibroblast growth factor (FGF8) and FGF10 identified in patients with conotruncal defects

doi: 10.1186/s12967-020-02445-2

Figure Lengend Snippet: Functional analysis of wild-type and mutated FGF8. a Relative amount of FGF8 in supernatants. b Effects of mutant and wild-type FGF8 on cell viability. c , d The variant effect of FGF8 on PEA3 expression (n = 3), GAPDH was used as an internal control

Article Snippet: The human FGF8 (pCMV6-Entry-FGF8, Myc-DDK-tagged) and FGF10 (pCMV6-Entry-FGF10, Myc-DDK-tagged) cDNA plasmids were purchased from Origene (Rockville, MD, USA).

Techniques: Functional Assay, Mutagenesis, Variant Assay, Expressing, Control

Journal: Journal of Translational Medicine

Article Title: Mutations in fibroblast growth factor (FGF8) and FGF10 identified in patients with conotruncal defects

doi: 10.1186/s12967-020-02445-2

Figure Lengend Snippet: Dynamic expression of FGF8 and FGF10 in human embryonic stem cells and human embryonic cardiac tissue. a , b Relative expression of FGF8 and FGF10 during the differentiation of human embryonic stem cells to cardiomyocytes. c Expression level of FGF8 and FGF10 in the human embryonic heart, d , e Immunohistochemistry of FGF8 and FGF10 in human embryos at Carnegie stge13

Article Snippet: The human FGF8 (pCMV6-Entry-FGF8, Myc-DDK-tagged) and FGF10 (pCMV6-Entry-FGF10, Myc-DDK-tagged) cDNA plasmids were purchased from Origene (Rockville, MD, USA).

Techniques: Expressing, Immunohistochemistry

Journal: Journal of Translational Medicine

Article Title: Mutations in fibroblast growth factor (FGF8) and FGF10 identified in patients with conotruncal defects

doi: 10.1186/s12967-020-02445-2

Figure Lengend Snippet: Scheme of the regulatory mechanisms involving FGF8 and FGF10 in the pathogenesis of CTDs. The upward arrow indicates increased expression, the downward arrow indicates decreased secretion, and the minus sign indicates inhibition

Article Snippet: The human FGF8 (pCMV6-Entry-FGF8, Myc-DDK-tagged) and FGF10 (pCMV6-Entry-FGF10, Myc-DDK-tagged) cDNA plasmids were purchased from Origene (Rockville, MD, USA).

Techniques: Expressing, Inhibition

Journal:

Article Title: Activin is an essential early mesenchymal signal in tooth development that is required for patterning of the murine dentition

doi:

Figure Lengend Snippet: FGF8 is required to maintain expression of activin βA expression in the odontogenic mesenchyme. (A,B) Serial frontal sections through the prospective molar region of an E11.5 wild-type showing the complementary expression patterns of activin βA in the mesenchyme (A) and Fgf8 in the epithelium (B) on consecutive sections. The tooth germ epithelium is outlined in red. (C,D) activin βA expression in E11.5 mandible explants that were cultured for 2 days with (C) and without (D) epithelium. The arrows indicate the positions of incisor buds. (E,F) activin βA expression in E11.5 mandible explants that were cultured for 1 day without epithelium but in the presence of implanted FGF8 beads (E) or BSA control beads (F). (G,H) DIG whole-mount in situ hybridization of E11.0 mandibles that were cultured without epithelium but in the presence of beads. (G) FGF8 maintains activin βA expression in the mesenchyme. Beads were placed on half of the mandible. (H) BMP4 does not inhibit the maintenance activity of FGF8. The experiment was performed on both halves of the mandible.

Article Snippet: For the application of FGF8 protein, heparin acrylic beads (Sigma) were washed and then incubated overnight in 1 mg/ml FGF8 protein (recombinant mouse FGF8b; R&D Systems, Abingdon, UK) at 4°C.

Techniques: Expressing, Cell Culture, In Situ Hybridization, Activity Assay