fgf Search Results


human recombinant fgf4  (MedChemExpress)
94
MedChemExpress human recombinant fgf4
(A) The morphology of ESCs, TBLCs and ESCs, TBLCs in TS medium after 3 days of induction. Scale bars, 250 μm. (B) FACS analysis of the percentage of CDX2 + cells from ESCs and TBLCs, as well as ESCs and TBLCs cultured in TS medium, using V6.5 cell line. (C) FACS analysis of the percentage of CD40 + cells from ESCs and TBLCs, as well as ESCs and TBLCs cultured in TS medium, using TC1 cell line. (D) FACS analysis of the percentage of CD40 + TELCs obtained from the TBLCs after induction with different molecules, including <t>FGF4,</t> Activin A, TGFβ1 and BMP4. The corresponding cell morphology is displayed in the lower panel. (E) Scatterplots displaying the transcriptome comparison of TELCs before and after CD40-based FACS using RNA-seq. Upregulated (FC>2) and downregulated (FC<0.5) genes are shown in red and blue, respectively. (F) The morphology of TBLCs of different passages and long-term culture in TX and TS medium, also the morphology of TBLCs after CD40 FACS after induction. Scale bars, 250 μm. (G) Western blotting was used to detect OCT4, CDX2 and EOMES in TELSCs from different passages. β-Tubulin was used as a loading control. (H) The morphology 8C embryos cultured in TX medium. Scale bars, 250 μm. (I) FACS analysis of the percentage of CD40 + cells in TELSC em s at different passages. (J) Immunofluorescence staining of TFAP2C and PEG10 in TBLCs, TELSCs and TELSC em s. Scale bars, 50 μm. (K) Cell cycle analysis of ESCs, TELSCs and TELSC em s. (L) Heatmap indicating the relative expression of TBLCs, TELSCs and TELSC em s. The representative genes and enrichment of GO terms of these genes is shown. (M) Heatmap indicating the relative expression of characteristic genes in TELSCs, TELSC em s and TSCs. Bubble chart showing the relative expression of these genes in mouse embryos. (N) Heatmap indicating the relative expression of characteristic genes in TELSCs, TSCs cultured in TX medium and TSCs cultured in TS medium. Heatmap on the right demonstrating the expression of each cluster in mouse embryos. The representative genes and enrichment of GO terms of these genes is shown. (O) The scatter plot displays differentially expressed genes between TELSCs and TSCs cultured in various media. The bar graph summarizes the number of differentially expressed genes identified under each comparison condition. (P) GSEA analysis of ESCs, TBLCs, TELCs and TELSCs based on “embryonic placenta development” and “placenta development” geneset. (Q) Heatmap indicating the differentially expressed genes in Hippo pathway of TELSCs and TBLCs. (R) Heatmap indicating the relative expression of characteristic genes in TELSCs, TSCs cultured in TX medium and TSCs cultured in TS medium. Bubble chart showing the relative expression of these genes in mouse embryos. (S) Phase contrast images of TBLCs cultured in TS medium for 24h supplemented with Verteporfin at the indicated concentration. Scale bars, 100 µm. (T) Heatmap indicating the differentially expressed genes of TELCs and TBLCs induction in TS medium plus verteporfin. Bubble chart showing the relative expression of these genes in mouse embryos. (U) GSEA analysis of TELCs, TBLCs induction in TS medium and in TS medium plus verteporfin based on TE geneset. (V) The morphology of TELSCs cultured in TS medium, TS medium plus ITS-X and TS medium plus TGFβ1. (W) Heatmap indicating the differentially expressed genes of TELSCs, TBLCs induction in TX medium withdraw ITS-X, in TS medium and in TS medium plus ITS-X. Heatmap on the right demonstrating the expression of each cluster in mouse embryos. The representative genes and enrichment of GO terms of these genes is shown. (X) GSEA analysis of TBLCs induction in TX medium withdraw ITS-X and in TX medium based on “Positive regulation of stem cell proliferation” and “Positive regulation of cell cycle” geneset.
Human Recombinant Fgf4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
human recombinant fgf4 - by Bioz Stars, 2026-03
94/100 stars
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human fgf 2  (Miltenyi Biotec)
95
Miltenyi Biotec human fgf 2
(A) The morphology of ESCs, TBLCs and ESCs, TBLCs in TS medium after 3 days of induction. Scale bars, 250 μm. (B) FACS analysis of the percentage of CDX2 + cells from ESCs and TBLCs, as well as ESCs and TBLCs cultured in TS medium, using V6.5 cell line. (C) FACS analysis of the percentage of CD40 + cells from ESCs and TBLCs, as well as ESCs and TBLCs cultured in TS medium, using TC1 cell line. (D) FACS analysis of the percentage of CD40 + TELCs obtained from the TBLCs after induction with different molecules, including <t>FGF4,</t> Activin A, TGFβ1 and BMP4. The corresponding cell morphology is displayed in the lower panel. (E) Scatterplots displaying the transcriptome comparison of TELCs before and after CD40-based FACS using RNA-seq. Upregulated (FC>2) and downregulated (FC<0.5) genes are shown in red and blue, respectively. (F) The morphology of TBLCs of different passages and long-term culture in TX and TS medium, also the morphology of TBLCs after CD40 FACS after induction. Scale bars, 250 μm. (G) Western blotting was used to detect OCT4, CDX2 and EOMES in TELSCs from different passages. β-Tubulin was used as a loading control. (H) The morphology 8C embryos cultured in TX medium. Scale bars, 250 μm. (I) FACS analysis of the percentage of CD40 + cells in TELSC em s at different passages. (J) Immunofluorescence staining of TFAP2C and PEG10 in TBLCs, TELSCs and TELSC em s. Scale bars, 50 μm. (K) Cell cycle analysis of ESCs, TELSCs and TELSC em s. (L) Heatmap indicating the relative expression of TBLCs, TELSCs and TELSC em s. The representative genes and enrichment of GO terms of these genes is shown. (M) Heatmap indicating the relative expression of characteristic genes in TELSCs, TELSC em s and TSCs. Bubble chart showing the relative expression of these genes in mouse embryos. (N) Heatmap indicating the relative expression of characteristic genes in TELSCs, TSCs cultured in TX medium and TSCs cultured in TS medium. Heatmap on the right demonstrating the expression of each cluster in mouse embryos. The representative genes and enrichment of GO terms of these genes is shown. (O) The scatter plot displays differentially expressed genes between TELSCs and TSCs cultured in various media. The bar graph summarizes the number of differentially expressed genes identified under each comparison condition. (P) GSEA analysis of ESCs, TBLCs, TELCs and TELSCs based on “embryonic placenta development” and “placenta development” geneset. (Q) Heatmap indicating the differentially expressed genes in Hippo pathway of TELSCs and TBLCs. (R) Heatmap indicating the relative expression of characteristic genes in TELSCs, TSCs cultured in TX medium and TSCs cultured in TS medium. Bubble chart showing the relative expression of these genes in mouse embryos. (S) Phase contrast images of TBLCs cultured in TS medium for 24h supplemented with Verteporfin at the indicated concentration. Scale bars, 100 µm. (T) Heatmap indicating the differentially expressed genes of TELCs and TBLCs induction in TS medium plus verteporfin. Bubble chart showing the relative expression of these genes in mouse embryos. (U) GSEA analysis of TELCs, TBLCs induction in TS medium and in TS medium plus verteporfin based on TE geneset. (V) The morphology of TELSCs cultured in TS medium, TS medium plus ITS-X and TS medium plus TGFβ1. (W) Heatmap indicating the differentially expressed genes of TELSCs, TBLCs induction in TX medium withdraw ITS-X, in TS medium and in TS medium plus ITS-X. Heatmap on the right demonstrating the expression of each cluster in mouse embryos. The representative genes and enrichment of GO terms of these genes is shown. (X) GSEA analysis of TBLCs induction in TX medium withdraw ITS-X and in TX medium based on “Positive regulation of stem cell proliferation” and “Positive regulation of cell cycle” geneset.
Human Fgf 2, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human fgf 2/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
human fgf 2 - by Bioz Stars, 2026-03
95/100 stars
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mouse anti phospho fgf receptor tyr653 654  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc mouse anti phospho fgf receptor tyr653 654
(A) The morphology of ESCs, TBLCs and ESCs, TBLCs in TS medium after 3 days of induction. Scale bars, 250 μm. (B) FACS analysis of the percentage of CDX2 + cells from ESCs and TBLCs, as well as ESCs and TBLCs cultured in TS medium, using V6.5 cell line. (C) FACS analysis of the percentage of CD40 + cells from ESCs and TBLCs, as well as ESCs and TBLCs cultured in TS medium, using TC1 cell line. (D) FACS analysis of the percentage of CD40 + TELCs obtained from the TBLCs after induction with different molecules, including <t>FGF4,</t> Activin A, TGFβ1 and BMP4. The corresponding cell morphology is displayed in the lower panel. (E) Scatterplots displaying the transcriptome comparison of TELCs before and after CD40-based FACS using RNA-seq. Upregulated (FC>2) and downregulated (FC<0.5) genes are shown in red and blue, respectively. (F) The morphology of TBLCs of different passages and long-term culture in TX and TS medium, also the morphology of TBLCs after CD40 FACS after induction. Scale bars, 250 μm. (G) Western blotting was used to detect OCT4, CDX2 and EOMES in TELSCs from different passages. β-Tubulin was used as a loading control. (H) The morphology 8C embryos cultured in TX medium. Scale bars, 250 μm. (I) FACS analysis of the percentage of CD40 + cells in TELSC em s at different passages. (J) Immunofluorescence staining of TFAP2C and PEG10 in TBLCs, TELSCs and TELSC em s. Scale bars, 50 μm. (K) Cell cycle analysis of ESCs, TELSCs and TELSC em s. (L) Heatmap indicating the relative expression of TBLCs, TELSCs and TELSC em s. The representative genes and enrichment of GO terms of these genes is shown. (M) Heatmap indicating the relative expression of characteristic genes in TELSCs, TELSC em s and TSCs. Bubble chart showing the relative expression of these genes in mouse embryos. (N) Heatmap indicating the relative expression of characteristic genes in TELSCs, TSCs cultured in TX medium and TSCs cultured in TS medium. Heatmap on the right demonstrating the expression of each cluster in mouse embryos. The representative genes and enrichment of GO terms of these genes is shown. (O) The scatter plot displays differentially expressed genes between TELSCs and TSCs cultured in various media. The bar graph summarizes the number of differentially expressed genes identified under each comparison condition. (P) GSEA analysis of ESCs, TBLCs, TELCs and TELSCs based on “embryonic placenta development” and “placenta development” geneset. (Q) Heatmap indicating the differentially expressed genes in Hippo pathway of TELSCs and TBLCs. (R) Heatmap indicating the relative expression of characteristic genes in TELSCs, TSCs cultured in TX medium and TSCs cultured in TS medium. Bubble chart showing the relative expression of these genes in mouse embryos. (S) Phase contrast images of TBLCs cultured in TS medium for 24h supplemented with Verteporfin at the indicated concentration. Scale bars, 100 µm. (T) Heatmap indicating the differentially expressed genes of TELCs and TBLCs induction in TS medium plus verteporfin. Bubble chart showing the relative expression of these genes in mouse embryos. (U) GSEA analysis of TELCs, TBLCs induction in TS medium and in TS medium plus verteporfin based on TE geneset. (V) The morphology of TELSCs cultured in TS medium, TS medium plus ITS-X and TS medium plus TGFβ1. (W) Heatmap indicating the differentially expressed genes of TELSCs, TBLCs induction in TX medium withdraw ITS-X, in TS medium and in TS medium plus ITS-X. Heatmap on the right demonstrating the expression of each cluster in mouse embryos. The representative genes and enrichment of GO terms of these genes is shown. (X) GSEA analysis of TBLCs induction in TX medium withdraw ITS-X and in TX medium based on “Positive regulation of stem cell proliferation” and “Positive regulation of cell cycle” geneset.
Mouse Anti Phospho Fgf Receptor Tyr653 654, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti phospho fgf receptor tyr653 654/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
mouse anti phospho fgf receptor tyr653 654 - by Bioz Stars, 2026-03
95/100 stars
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rabbit polyclonal anti fgf10 antibody  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology rabbit polyclonal anti fgf10 antibody
Figure 5. <t>Fgf10-induced</t> IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.
Rabbit Polyclonal Anti Fgf10 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
rabbit polyclonal anti fgf10 antibody - by Bioz Stars, 2026-03
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fgf receptor 1 d8e4  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc fgf receptor 1 d8e4
Figure 5. <t>Fgf10-induced</t> IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.
Fgf Receptor 1 D8e4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fgf receptor 1 d8e4/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
fgf receptor 1 d8e4 - by Bioz Stars, 2026-03
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235 f4 025  (R&D Systems)
95
R&D Systems 235 f4 025
Figure 5. <t>Fgf10-induced</t> IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.
235 F4 025, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/235 f4 025/product/R&D Systems
Average 95 stars, based on 1 article reviews
235 f4 025 - by Bioz Stars, 2026-03
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buffer  (Proteintech)
94
Proteintech buffer
Figure 5. <t>Fgf10-induced</t> IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.
Buffer, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer/product/Proteintech
Average 94 stars, based on 1 article reviews
buffer - by Bioz Stars, 2026-03
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fgf2 recombinant protein  (MedChemExpress)
94
MedChemExpress fgf2 recombinant protein
Figure 5. <t>Fgf10-induced</t> IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.
Fgf2 Recombinant Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fgf2 recombinant protein/product/MedChemExpress
Average 94 stars, based on 1 article reviews
fgf2 recombinant protein - by Bioz Stars, 2026-03
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weight method art  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology weight method art
Figure 5. <t>Fgf10-induced</t> IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.
Weight Method Art, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/weight method art/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
weight method art - by Bioz Stars, 2026-03
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fgf10  (R&D Systems)
94
R&D Systems fgf10
Figure 5. <t>Fgf10-induced</t> IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.
Fgf10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fgf10/product/R&D Systems
Average 94 stars, based on 1 article reviews
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rabbit polyclonal anti fgf receptor 4  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc rabbit polyclonal anti fgf receptor 4
Figure 5. <t>Fgf10-induced</t> IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.
Rabbit Polyclonal Anti Fgf Receptor 4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti fgf receptor 4/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
rabbit polyclonal anti fgf receptor 4 - by Bioz Stars, 2026-03
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Image Search Results


(A) The morphology of ESCs, TBLCs and ESCs, TBLCs in TS medium after 3 days of induction. Scale bars, 250 μm. (B) FACS analysis of the percentage of CDX2 + cells from ESCs and TBLCs, as well as ESCs and TBLCs cultured in TS medium, using V6.5 cell line. (C) FACS analysis of the percentage of CD40 + cells from ESCs and TBLCs, as well as ESCs and TBLCs cultured in TS medium, using TC1 cell line. (D) FACS analysis of the percentage of CD40 + TELCs obtained from the TBLCs after induction with different molecules, including FGF4, Activin A, TGFβ1 and BMP4. The corresponding cell morphology is displayed in the lower panel. (E) Scatterplots displaying the transcriptome comparison of TELCs before and after CD40-based FACS using RNA-seq. Upregulated (FC>2) and downregulated (FC<0.5) genes are shown in red and blue, respectively. (F) The morphology of TBLCs of different passages and long-term culture in TX and TS medium, also the morphology of TBLCs after CD40 FACS after induction. Scale bars, 250 μm. (G) Western blotting was used to detect OCT4, CDX2 and EOMES in TELSCs from different passages. β-Tubulin was used as a loading control. (H) The morphology 8C embryos cultured in TX medium. Scale bars, 250 μm. (I) FACS analysis of the percentage of CD40 + cells in TELSC em s at different passages. (J) Immunofluorescence staining of TFAP2C and PEG10 in TBLCs, TELSCs and TELSC em s. Scale bars, 50 μm. (K) Cell cycle analysis of ESCs, TELSCs and TELSC em s. (L) Heatmap indicating the relative expression of TBLCs, TELSCs and TELSC em s. The representative genes and enrichment of GO terms of these genes is shown. (M) Heatmap indicating the relative expression of characteristic genes in TELSCs, TELSC em s and TSCs. Bubble chart showing the relative expression of these genes in mouse embryos. (N) Heatmap indicating the relative expression of characteristic genes in TELSCs, TSCs cultured in TX medium and TSCs cultured in TS medium. Heatmap on the right demonstrating the expression of each cluster in mouse embryos. The representative genes and enrichment of GO terms of these genes is shown. (O) The scatter plot displays differentially expressed genes between TELSCs and TSCs cultured in various media. The bar graph summarizes the number of differentially expressed genes identified under each comparison condition. (P) GSEA analysis of ESCs, TBLCs, TELCs and TELSCs based on “embryonic placenta development” and “placenta development” geneset. (Q) Heatmap indicating the differentially expressed genes in Hippo pathway of TELSCs and TBLCs. (R) Heatmap indicating the relative expression of characteristic genes in TELSCs, TSCs cultured in TX medium and TSCs cultured in TS medium. Bubble chart showing the relative expression of these genes in mouse embryos. (S) Phase contrast images of TBLCs cultured in TS medium for 24h supplemented with Verteporfin at the indicated concentration. Scale bars, 100 µm. (T) Heatmap indicating the differentially expressed genes of TELCs and TBLCs induction in TS medium plus verteporfin. Bubble chart showing the relative expression of these genes in mouse embryos. (U) GSEA analysis of TELCs, TBLCs induction in TS medium and in TS medium plus verteporfin based on TE geneset. (V) The morphology of TELSCs cultured in TS medium, TS medium plus ITS-X and TS medium plus TGFβ1. (W) Heatmap indicating the differentially expressed genes of TELSCs, TBLCs induction in TX medium withdraw ITS-X, in TS medium and in TS medium plus ITS-X. Heatmap on the right demonstrating the expression of each cluster in mouse embryos. The representative genes and enrichment of GO terms of these genes is shown. (X) GSEA analysis of TBLCs induction in TX medium withdraw ITS-X and in TX medium based on “Positive regulation of stem cell proliferation” and “Positive regulation of cell cycle” geneset.

Journal: bioRxiv

Article Title: Capturing trophectoderm-like stem cells enables step-wisely remodeling of placental development

doi: 10.1101/2025.08.25.672082

Figure Lengend Snippet: (A) The morphology of ESCs, TBLCs and ESCs, TBLCs in TS medium after 3 days of induction. Scale bars, 250 μm. (B) FACS analysis of the percentage of CDX2 + cells from ESCs and TBLCs, as well as ESCs and TBLCs cultured in TS medium, using V6.5 cell line. (C) FACS analysis of the percentage of CD40 + cells from ESCs and TBLCs, as well as ESCs and TBLCs cultured in TS medium, using TC1 cell line. (D) FACS analysis of the percentage of CD40 + TELCs obtained from the TBLCs after induction with different molecules, including FGF4, Activin A, TGFβ1 and BMP4. The corresponding cell morphology is displayed in the lower panel. (E) Scatterplots displaying the transcriptome comparison of TELCs before and after CD40-based FACS using RNA-seq. Upregulated (FC>2) and downregulated (FC<0.5) genes are shown in red and blue, respectively. (F) The morphology of TBLCs of different passages and long-term culture in TX and TS medium, also the morphology of TBLCs after CD40 FACS after induction. Scale bars, 250 μm. (G) Western blotting was used to detect OCT4, CDX2 and EOMES in TELSCs from different passages. β-Tubulin was used as a loading control. (H) The morphology 8C embryos cultured in TX medium. Scale bars, 250 μm. (I) FACS analysis of the percentage of CD40 + cells in TELSC em s at different passages. (J) Immunofluorescence staining of TFAP2C and PEG10 in TBLCs, TELSCs and TELSC em s. Scale bars, 50 μm. (K) Cell cycle analysis of ESCs, TELSCs and TELSC em s. (L) Heatmap indicating the relative expression of TBLCs, TELSCs and TELSC em s. The representative genes and enrichment of GO terms of these genes is shown. (M) Heatmap indicating the relative expression of characteristic genes in TELSCs, TELSC em s and TSCs. Bubble chart showing the relative expression of these genes in mouse embryos. (N) Heatmap indicating the relative expression of characteristic genes in TELSCs, TSCs cultured in TX medium and TSCs cultured in TS medium. Heatmap on the right demonstrating the expression of each cluster in mouse embryos. The representative genes and enrichment of GO terms of these genes is shown. (O) The scatter plot displays differentially expressed genes between TELSCs and TSCs cultured in various media. The bar graph summarizes the number of differentially expressed genes identified under each comparison condition. (P) GSEA analysis of ESCs, TBLCs, TELCs and TELSCs based on “embryonic placenta development” and “placenta development” geneset. (Q) Heatmap indicating the differentially expressed genes in Hippo pathway of TELSCs and TBLCs. (R) Heatmap indicating the relative expression of characteristic genes in TELSCs, TSCs cultured in TX medium and TSCs cultured in TS medium. Bubble chart showing the relative expression of these genes in mouse embryos. (S) Phase contrast images of TBLCs cultured in TS medium for 24h supplemented with Verteporfin at the indicated concentration. Scale bars, 100 µm. (T) Heatmap indicating the differentially expressed genes of TELCs and TBLCs induction in TS medium plus verteporfin. Bubble chart showing the relative expression of these genes in mouse embryos. (U) GSEA analysis of TELCs, TBLCs induction in TS medium and in TS medium plus verteporfin based on TE geneset. (V) The morphology of TELSCs cultured in TS medium, TS medium plus ITS-X and TS medium plus TGFβ1. (W) Heatmap indicating the differentially expressed genes of TELSCs, TBLCs induction in TX medium withdraw ITS-X, in TS medium and in TS medium plus ITS-X. Heatmap on the right demonstrating the expression of each cluster in mouse embryos. The representative genes and enrichment of GO terms of these genes is shown. (X) GSEA analysis of TBLCs induction in TX medium withdraw ITS-X and in TX medium based on “Positive regulation of stem cell proliferation” and “Positive regulation of cell cycle” geneset.

Article Snippet: All TSLs were cultured on Matrigel-coated plates, in 30% TS medium (RPMI 1640 (GIBCO, 11875119), 20% FBS, 1% GlutaMax (GIBCO, 35050061), 1% penicillin-streptomycin (GIBCO, 15140163), 1% sodium pyruvate (GIBCO, 11360070)) and 70% MEF-conditioned TS medium supplemented with 25 ng/ml human recombinant FGF4 (MCE, HY-P7014) and 1 μg/ml heparin (STEMCELL, 7980).

Techniques: Cell Culture, Comparison, RNA Sequencing, Western Blot, Control, Immunofluorescence, Staining, Cell Cycle Assay, Expressing, Concentration Assay

Figure 5. Fgf10-induced IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.

Journal: Cell reports

Article Title: Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas.

doi: 10.1016/j.celrep.2021.108772

Figure Lengend Snippet: Figure 5. Fgf10-induced IPNB shows stepwise carcinogenesis (A) Schematic representation of iFGF10: LSL-KrasG12D:Alb-Cre or Pdx1-Cre (iFGF10KA or iFGF10KP, respectively) mice. (B) H&E images of IPNB lesion in iFGF10KA (Kras+) and iFGF10A (Kras) mice following 0.002% Dox administration for 6 weeks (N = 5 each). Insets indicate high magnification of the rectangles.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Monoclonal Anti-Human Cytokeratin (clones AE1/AE3) DAKO Cat# M3515 LOT: 10066159 Rabbit Polyclonal Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Cell Signaling Technology Cat# 4370 RRID:AB_2315112 Rabbit polyclonal Anti-alpha smooth muscle Actin [1A4] Abcam Cat# ab7817 Rabbit polyclonal Anti-S100 antibody Abcam Cat# ab166649 Rabbit polyclonal Anti-FGF10 antibody (for mouse) Santa Cruz Cat# sc-293208 Rabbit polyclonal Anti-FGF10 antibody (for human) Abcam Cat# ab80064 Rabbit polyclonal Anti-FGF10 antibody Sigma-Aldrich Cat#ABN44 Mouse monoclonal Anti-MUC5AC antibody Abcam Cat# ab3649 Mouse monoclonal Anti-MUC6 antibody Leica Biosystems Cat# 130307 Rabbit polyclonal Anti-MUC2 antibody Novus Biologicals Cat# NBP1-31231 Rabbit monoclonal Anti-MUC1 antibody Invitrogen Cat# MA5-35250 Rabbit polyclonal Anti-Phospho-FGFR Pan (Tyr653, Tyr654) antibody Invitrogen Cat# PA5-105938 Rabbit polyclonal Anti-Src (phospho Y418) antibody Abcam Cat# ab4816 Rabbit Polyclonal RFP Antibody Preadsorbed Rockland Immunochemicals Cat# 600-401-379 Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed Abcam Cat# ab150117 Goat Anti-Rabbit IgG H&L (Alexa Fluor 594) preadsorbed Abcam Cat# ab150084 Rabbit IgG (Control Antibody) Vector Laboratories Cat# I-1000 Mouse IgG (Control Antibody) Vector Laboratories Cat# I-2000 Bacterial and virus strains Escherichia coli strain SW105 Frederick National Laboratory Cancer Research, NCI NA Biological samples Human biliary tumor samples This paper NA Chemicals, peptides, and recombinant proteins Tamoxifen Sigma Cat# T5648 Recommbinant mouse FGF10 R&D Systems Cat# 6224-FG Recommbinant human FGF10 R&D Systems Cat# 345-FG-025 thioacetamide Sigma Cat# 163678 Doxycycline hyclate Sigma Cat# D9891 Polyinosinic-Polycytidylic acid Sigma Cat# P1530 - Cellstain - DAPI solution DOJINDO Cat# D523 PD0325901 Selleck Chemicals Cat# S1036 L-arabinose Sigma Cat# A3256 Sucrose FUJIFILM Cat# 192-00017 dimethyl sulfoxide FUJIFILM Cat# 043-07211 (Continued on next page) Cell Reports 34, 108772, February 23, 2021 e1

Techniques:

Figure 6. Fgf10 is required for maintaining papillary structure defined as IPNB (A) Experimental design of the withdrawal of Dox and/or TAA administration to iFGF10 mice: (1) Dox-only (Dox+) administration and (2) TAA+Dox+ administration followed by the withdrawal (OFF) (N = 5 each). ‘‘IPNB establishment time’’ and ‘‘endpoint time’’ are indicated.

Journal: Cell reports

Article Title: Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas.

doi: 10.1016/j.celrep.2021.108772

Figure Lengend Snippet: Figure 6. Fgf10 is required for maintaining papillary structure defined as IPNB (A) Experimental design of the withdrawal of Dox and/or TAA administration to iFGF10 mice: (1) Dox-only (Dox+) administration and (2) TAA+Dox+ administration followed by the withdrawal (OFF) (N = 5 each). ‘‘IPNB establishment time’’ and ‘‘endpoint time’’ are indicated.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Monoclonal Anti-Human Cytokeratin (clones AE1/AE3) DAKO Cat# M3515 LOT: 10066159 Rabbit Polyclonal Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Cell Signaling Technology Cat# 4370 RRID:AB_2315112 Rabbit polyclonal Anti-alpha smooth muscle Actin [1A4] Abcam Cat# ab7817 Rabbit polyclonal Anti-S100 antibody Abcam Cat# ab166649 Rabbit polyclonal Anti-FGF10 antibody (for mouse) Santa Cruz Cat# sc-293208 Rabbit polyclonal Anti-FGF10 antibody (for human) Abcam Cat# ab80064 Rabbit polyclonal Anti-FGF10 antibody Sigma-Aldrich Cat#ABN44 Mouse monoclonal Anti-MUC5AC antibody Abcam Cat# ab3649 Mouse monoclonal Anti-MUC6 antibody Leica Biosystems Cat# 130307 Rabbit polyclonal Anti-MUC2 antibody Novus Biologicals Cat# NBP1-31231 Rabbit monoclonal Anti-MUC1 antibody Invitrogen Cat# MA5-35250 Rabbit polyclonal Anti-Phospho-FGFR Pan (Tyr653, Tyr654) antibody Invitrogen Cat# PA5-105938 Rabbit polyclonal Anti-Src (phospho Y418) antibody Abcam Cat# ab4816 Rabbit Polyclonal RFP Antibody Preadsorbed Rockland Immunochemicals Cat# 600-401-379 Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed Abcam Cat# ab150117 Goat Anti-Rabbit IgG H&L (Alexa Fluor 594) preadsorbed Abcam Cat# ab150084 Rabbit IgG (Control Antibody) Vector Laboratories Cat# I-1000 Mouse IgG (Control Antibody) Vector Laboratories Cat# I-2000 Bacterial and virus strains Escherichia coli strain SW105 Frederick National Laboratory Cancer Research, NCI NA Biological samples Human biliary tumor samples This paper NA Chemicals, peptides, and recombinant proteins Tamoxifen Sigma Cat# T5648 Recommbinant mouse FGF10 R&D Systems Cat# 6224-FG Recommbinant human FGF10 R&D Systems Cat# 345-FG-025 thioacetamide Sigma Cat# 163678 Doxycycline hyclate Sigma Cat# D9891 Polyinosinic-Polycytidylic acid Sigma Cat# P1530 - Cellstain - DAPI solution DOJINDO Cat# D523 PD0325901 Selleck Chemicals Cat# S1036 L-arabinose Sigma Cat# A3256 Sucrose FUJIFILM Cat# 192-00017 dimethyl sulfoxide FUJIFILM Cat# 043-07211 (Continued on next page) Cell Reports 34, 108772, February 23, 2021 e1

Techniques: