fe2 Search Results


fluorescent probe sirhonox 1  (GORYO Chemical)
94
GORYO Chemical fluorescent probe sirhonox 1
TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized <t>with</t> <t>SiRhoNox-1</t> staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Fluorescent Probe Sirhonox 1, supplied by GORYO Chemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ferrous iron  (Novus Biologicals)
94
Novus Biologicals ferrous iron
TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized <t>with</t> <t>SiRhoNox-1</t> staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Ferrous Iron, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell ferrous iron fe2 fluorometric assay kit  (Elabscience Biotechnology)
95
Elabscience Biotechnology cell ferrous iron fe2 fluorometric assay kit
TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized <t>with</t> <t>SiRhoNox-1</t> staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Cell Ferrous Iron Fe2 Fluorometric Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent probe  (GORYO Chemical)
95
GORYO Chemical fluorescent probe
TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized <t>with</t> <t>SiRhoNox-1</t> staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Fluorescent Probe, supplied by GORYO Chemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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statistics package combistats 5 0  (European Directorate for the Quality of Medicines and HealthCare)
88
European Directorate for the Quality of Medicines and HealthCare statistics package combistats 5 0
TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized <t>with</t> <t>SiRhoNox-1</t> staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Statistics Package Combistats 5 0, supplied by European Directorate for the Quality of Medicines and HealthCare, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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statistics package combistats 5 0 - by Bioz Stars, 2026-06
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iron(iii) oxide fe 2 o 3  (InnoChem Inc)
90
InnoChem Inc iron(iii) oxide fe 2 o 3
TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized <t>with</t> <t>SiRhoNox-1</t> staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Iron(iii) Oxide Fe 2 O 3, supplied by InnoChem Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fe 2 o 3 nps  (NanoSany Corporation)
90
NanoSany Corporation fe 2 o 3 nps
TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized <t>with</t> <t>SiRhoNox-1</t> staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Fe 2 O 3 Nps, supplied by NanoSany Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fe 2 (so 4 ) 3  (Avantor)
90
Avantor fe 2 (so 4 ) 3
TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized <t>with</t> <t>SiRhoNox-1</t> staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Fe 2 (So 4 ) 3, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chro) 2 -fe 2  (ACGT Inc)
90
ACGT Inc chro) 2 -fe 2
TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized <t>with</t> <t>SiRhoNox-1</t> staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Chro) 2 Fe 2, supplied by ACGT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chro) 2 -fe 2 - by Bioz Stars, 2026-06
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fe2(so4)3 solution  (Corning Life Sciences)
90
Corning Life Sciences fe2(so4)3 solution
TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized <t>with</t> <t>SiRhoNox-1</t> staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Fe2(so4)3 Solution, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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excess fe 2  (Verlag GmbH)
90
Verlag GmbH excess fe 2
TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized <t>with</t> <t>SiRhoNox-1</t> staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Excess Fe 2, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pt:fe 2 o 3  (SAS institute)
90
SAS institute pt:fe 2 o 3
TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized <t>with</t> <t>SiRhoNox-1</t> staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Pt:Fe 2 O 3, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized with SiRhoNox-1 staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

Journal: Journal of Orthopaedic Translation

Article Title: TRIM59 alleviates neuronal ferroptosis and promotes functional recovery after spinal cord injury by mediating ubiquitination and degradation of ANXA2

doi: 10.1016/j.jot.2026.101070

Figure Lengend Snippet: TRIM59 mitigates ferroptosis in vitro . Neuronal cells were transfected with Lv-shTRIM59 or Lv-TRIM59 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B, C) Transfection efficiency was confirmed through western blot analysis (n = 3); (D) Cell viability was determined using CCK-8 assay (n = 6); (E, F) Neuronal survival was examined by Calcein-AM/PI double staining (n = 3); (G-K) ROS, Fe 2+ , MDA, and GSH levels were measured (n = 3); (L, M) Fe 2+ accumulation was visualized with SiRhoNox-1 staining (n = 3); (N) Ferroptosis-related protein expression was assessed by western blot (n = 3); (O, P) Mitochondrial ROS accumulation was measured using MitoSOX immunofluorescence and corresponding statistical analysis (n = 3); (Q, R) Mitochondrial membrane potential was assessed via JC-1 staining, with quantification of JC-1 aggregates and monomers (n = 3); (S) Representative TEM images of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

Article Snippet: Intracellular labile iron pool (Fe 2+ ) levels were detected using the fluorescent probe SiRhoNox-1 (FerroFarRed, Goryo Chemical Japan), as previously described [ ].

Techniques: In Vitro, Transfection, Western Blot, CCK-8 Assay, Double Staining, Staining, Expressing, Immunofluorescence, Membrane

Lv-shANXA2 mitigates the ferroptosis induced by TRIM59 knockdown. Neuronal cells were transfected with either Lv-shANXA2 or Lv-ANXA2 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B) Cell viability assessment via CCK-8 assay (n = 6); (C, D) Neuronal survival evaluation through Calcein-AM/PI staining (n = 3); (E, F) Intracellular ROS quantification by flow cytometry with statistical analysis (n = 3); (G-I) Measurement of Fe 2+ , MDA, and GSH levels in neuronal cells (n = 3); (J, K) Fe 2+ accumulation detection using SiRhoNox-1 staining (n = 3); (L) Western blot examination of ferroptosis-associated protein expression in neuronal cells (n = 3); (M, N) Mitochondrial ROS detection and quantification via MitoSOX immunofluorescence in neuronal cells (n = 3); (O, P) Mitochondrial membrane potential assessment through JC-1 staining, including JC-1 aggregate-to-monomer ratio quantification (n = 3); (Q) Transmission electron microscopy visualization of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

Journal: Journal of Orthopaedic Translation

Article Title: TRIM59 alleviates neuronal ferroptosis and promotes functional recovery after spinal cord injury by mediating ubiquitination and degradation of ANXA2

doi: 10.1016/j.jot.2026.101070

Figure Lengend Snippet: Lv-shANXA2 mitigates the ferroptosis induced by TRIM59 knockdown. Neuronal cells were transfected with either Lv-shANXA2 or Lv-ANXA2 and treated with Erastin (5 μM) for 6 h. (A) Experimental group specifications; (B) Cell viability assessment via CCK-8 assay (n = 6); (C, D) Neuronal survival evaluation through Calcein-AM/PI staining (n = 3); (E, F) Intracellular ROS quantification by flow cytometry with statistical analysis (n = 3); (G-I) Measurement of Fe 2+ , MDA, and GSH levels in neuronal cells (n = 3); (J, K) Fe 2+ accumulation detection using SiRhoNox-1 staining (n = 3); (L) Western blot examination of ferroptosis-associated protein expression in neuronal cells (n = 3); (M, N) Mitochondrial ROS detection and quantification via MitoSOX immunofluorescence in neuronal cells (n = 3); (O, P) Mitochondrial membrane potential assessment through JC-1 staining, including JC-1 aggregate-to-monomer ratio quantification (n = 3); (Q) Transmission electron microscopy visualization of mitochondrial ultrastructure in neuronal cells. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

Article Snippet: Intracellular labile iron pool (Fe 2+ ) levels were detected using the fluorescent probe SiRhoNox-1 (FerroFarRed, Goryo Chemical Japan), as previously described [ ].

Techniques: Knockdown, Transfection, CCK-8 Assay, Staining, Flow Cytometry, Western Blot, Expressing, Immunofluorescence, Membrane, Transmission Assay, Electron Microscopy