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Image Search Results
Journal: BMC Developmental Biology
Article Title: Loss of muscleblind-like 1 promotes invasive mesenchyme formation in endocardial cushions by stimulating autocrine TGFβ3
doi: 10.1186/1471-213X-12-22
Figure Lengend Snippet: Addition of exogenous TGFβ3 recapitulates the effects of MBNL1 knockdown. Stage 14 AVC explants were treated with M199 supplemented with 0 to 200 ng/ml recombinant TGFβ3 at 6 hours. ( A ) The number of invaded cells was counted at 38 hrs. The mean number of invaded cells per explant + the standard error of the mean is shown. Addition of rTGFβ3 induced an increase in cell invasion, comparable at the highest dose tested to that seen following MBNL1 siRNA treatment. ( B ) The myocardium was removed and total RNA was harvested at 38 hrs. Expression of the mesenchymal markers ACTA2 and FBN2 were measured by real-time RT-PCR. Error bars denote 95% confidence intervals. Although the extent of ACTA2 and FBN2 up-regulation was somewhat variable, both markers were elevated in each of the pooled samples for all three doses (individual data not shown). An asterisk indicates a significant difference from 0 ng/ml rTGFβ3 (P ≤ 0.05).
Article Snippet: Real-time PCR reactions were carried out using TaqMan Gene Expression Assays on the StepOnePlus platform (
Techniques: Knockdown, Recombinant, Expressing, Quantitative RT-PCR
Journal: Fujita Medical Journal
Article Title: Phosphate overload via the type III Na-dependent Pi transporter represses aortic wall elastic fiber formation
doi: 10.20407/fmj.2023-004
Figure Lengend Snippet: Col1a1 , Eln , Fbn1 , Fbn2 , MGP , and Runx-2 gene expression in thoracic descending aortae of type III phosphate transporter transgenic (TG) rats (n=6) (open bar) and their wild-type littermates (WT) (n=6) (closed bar) at 8 weeks old. (A) Col1a1 , (B) Eln , (C) Fbn1 , (D) Fbn2 , (E) MGP , and (F) Runx-2 ). * p <0.05 was considered significant.
Article Snippet: The assay IDs of TaqMan probes used for qRT-PCR were as follows: rat collagen type 1 ( Col1a1 ), Rn01463848_m1; rat elastin ( Eln ), Rn01499782_m1; rat fibrillin-1 ( Fbn1 ), Rn01514895_m1; rat fibrillin-2 ( Fbn2 ),
Techniques: Gene Expression, Transgenic Assay