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Image Search Results
Journal: Nature Communications
Article Title: Complement receptor CD46 co-stimulates optimal human CD8 + T cell effector function via fatty acid metabolism
doi: 10.1038/s41467-018-06706-z
Figure Lengend Snippet: CD46 co-stimulation augments nutrient influx and fatty-acid synthesis. a , b Differentially expressed genes in CD8 + T cells of four healthy donors (HD) and three CD46-deficient (CD46-def.) patients (CD46-2 to -4) upon CD3 + CD46 activation at 6 h ( a ) and Venn diagram indicating overlap between differentially expressed transcripts ( b ). c Heatmaps comparing non-activated (NA) and CD3 + CD46-activated (6 h) HD CTLs with focus on genes involved in lipid/lipoprotein metabolism (left), transmembrane solute transport pathways (right), and FABP5 (left below). d Effect of CD46 co-stimulation on LAT1 and FASN expression and on mTOR activation. CD8 + T cells (gated as in Supplementary Fig. ; gray histograms, isotype controls; black histograms, non-activated cells; blue, green, or red histograms, CD3, CD3 + CD28, or CD3 + CD46 activation, respectively) from HDs were left NA or stimulated as depicted and LAT1 and FASN expression and mTOR activity (70S6K phosphorylation) measured 48 h post activation ( n = 4). e Effect of FASN inhibition on IFN-γ production and degranulation of CTLs. CD8 + T cells were activated as in ( d ) in the presence or absence of the FASN inhibitor C75 and IFN-γ secretion and CD107a expression measured at 48 h post activation ( n = 3–4; gray bars, carrier addition; white bars, addition of 5 μM C75; black bars, addition of 10 μM C75). f Heatmap indicating FASN, FABP5 and SLC7A5 expression in HD ( n = 4) vs. CD46-deficient ( n = 3) CD8 + T cells in resting state and after CD3 + CD46 activation. g RT-PCR for FASN , FABP5 , and SLC7A5 in CD3 or CD3 + CD46-activated CD8 + T cells from patients CD46-1, CD46-2, and CD46-4 (light red bars), and three matched HDs (light blue bars) at 6 h post activation ( n = 3). Error bars denote mean ± SEM. * p < 0.05; ** p < 0.01. Statistical analyses were performed using One-way ANOVA with Tukey Multiple Comparison test or the Paired Student’s t -test where appropriate
Article Snippet: FASN (Hs01005622_m1; http://www.ncbi.nlm.nih.gov/nuccore/NM_004104.4?report = fasta&log$ = seqview&format = text&from = 249&to = 549 ), FABP5 (
Techniques: Activation Assay, Expressing, Activity Assay, Phospho-proteomics, Inhibition, Reverse Transcription Polymerase Chain Reaction, Comparison