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Image Search Results
Journal: PLoS ONE
Article Title: Networked T Cell Death following Macrophage Infection by Mycobacterium tuberculosis
doi: 10.1371/journal.pone.0038488
Figure Lengend Snippet: (A) Addition of the pan-caspase inhibitor Z-VAD-FMK mitigated cell death induced by low MOI and high MOI-infected macrophage supernatants (shaded bars, infection + and ++ respectively), as well as background cell death observed in the uninfected control supernatants (closed bars, infection -). Cell death in infected supernatants was not mitigated by coincubation with blocking antibodies to TNF-α (B) or Fas (C), and in each case was significantly higher than in uninfected control supernatants (closed bars). Cell death induced by positive controls (D) (1 µM Staurosporine, 5 ng/ml recombinant human TNF-α +0.2 µg/ml cycloheximide and 10 ng/ml recombinant human Fas ligand) was in each case significantly reduced by coincubation with the corresponding inhibitor (open bars). Data shown in each panel are from one representative experiment, showing mean percentage cell death ± SEM from triplicate incubations. ** = p<0.01, *** = p<0.001, Student’s t test relative to uninfected control (A–C), or positive control relative to inhibitor (D).
Article Snippet: In positive control experiments for Fas ligand-mediated cell killing, cells were incubated in the presence of 10 ng/ml
Techniques: Infection, Control, Blocking Assay, Recombinant, Positive Control