Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: CD95 , REA738 , 50 , 130-113-004 , PE (FITC) , Miltenyi Biotec.

Techniques: Imaging

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: T cell subset classification and spatial distribution in an HCC sample. (A) Bar graph quantifications of gated T cell CD3 + T cells subpopulations (CD4 + T h , CD8 + T c , FoxP3 + /CD25 + T reg ) as well as T cell differentiation subsets (T N , T SCM , T CM , T EM , T EMRA , T TE ) for HCC tumor margin and tumor core. (B) Subclassification criteria used for T cell differentiation subset gating. Detailed gating scheme see Supplementary Figures S5A–D Spatial distribution of CD3+ T cell subsets as well as CD4+ and CD8+ differentiation subsets for tumor margin (C) and tumor core (D) . Arrowheads in (C, D) highlight the spatial distribution of regulatory T cells (T reg ). (E) Violin plots demonstrating expression levels of markers used for the definition of T cell differentiation subsets in tumor margin and tumor core: CD25, FoxP3, CD45RA, CD45RO, CD27, CD95, CD11a. Depicted markers and annotated cell types as indicated by the color code. ROI sizes: Tumor margin (ROI15) and tumor core (ROI16): 975 x 769 µm.

Article Snippet: CD95 , REA738 , 50 , 130-113-004 , PE (FITC) , Miltenyi Biotec.

Techniques: Cell Differentiation, Expressing