Journal: PLoS ONE

Article Title: Inverse Immunological Responses Induced by Allergic Rhinitis and Head and Neck Squamous Cell Carcinoma

doi: 10.1371/journal.pone.0086796

Figure Lengend Snippet: Blood was obtained from healthy controls (n = 10), patients with an ongoing seasonal allergic rhinitis (AR; n = 11) and patients with HNSCC (ELISA n = 9; FACS n = 10). PMN were isolated and cultured in the presence or absence of Pam 3 CSK 4 (1 µg/ml), LPS (1 µg/ml), R837 (5 µg/ml) or CpG (0.3 µM) for 4 and 24 h. ( A ) The cell free supernatants were then analyzed for IL-6 and IL-8 with ELISA, ( B ) and the CD16 positive cells were investigated for the expression of CD11b and CD69 using flow cytometry. The results are presented as the non-stimulated value minus the TLR stimulated values. Grey colored samples were analyzed on a BD LSRFortessa, whereas the rest of the samples were investigated on a Beckman Coulter Navios flow cytometer. ( C ) PMN from healthy individuals (n = 9) were incubated with or without culture medium supernatant from the HNSCC cell line FaDu, and stimulated with or without 1 µg/ml LPS for 4 h, and analyzed for IL-6 and IL-8 secretion with ELISA. The basal cytokine levels in the media were subtracted from the concentrations present in the PMN cultures. MFI = mean fluorescence intensity; * p ≤0.05; ** p ≤0.01; *** p ≤0.001.

Article Snippet: The human pharyngeal squamous cell carcinoma cell line FaDu (HTB-43; ATCC; Manassa, VA) was cultured in minimum essential medium (MEM) with Earl’s salts and 2 mM L-glutamine (GIBCO, Grand Island, NY), and supplemented with 10% FBS (PAN Biotech, Aidenbach, Germany), and 100 U/ml penicillin and 100 μg/ml streptomycin (GIBCO).

Techniques: Enzyme-linked Immunosorbent Assay, Isolation, Cell Culture, Expressing, Flow Cytometry, Incubation, Fluorescence

Journal: Frontiers in Pharmacology

Article Title: Pharmacological impact of microRNAs in head and neck squamous cell carcinoma: Prevailing insights on molecular pathways, diagnosis, and nanomedicine treatment

doi: 10.3389/fphar.2023.1174330

Figure Lengend Snippet: Summary of miRNAs dysregulated in HNSCC.

Article Snippet: miR-29s , HNSCC , Healthy tissues and entangled tumors , 23 patients , FaDU and SAS , ITGA6 and LAMC2 , Focal adhesion cascade , miR-29s inhibits cancers cell motility by regulating laminin-integrin signaling , --- , .

Techniques: Clinical Proteomics, Control, Inhibition, Expressing, Over Expression

Journal: Frontiers in Pharmacology

Article Title: Pharmacological impact of microRNAs in head and neck squamous cell carcinoma: Prevailing insights on molecular pathways, diagnosis, and nanomedicine treatment

doi: 10.3389/fphar.2023.1174330

Figure Lengend Snippet: Summary of miRNAs upregulated in HNSCC.

Article Snippet: miR-29s , HNSCC , Healthy tissues and entangled tumors , 23 patients , FaDU and SAS , ITGA6 and LAMC2 , Focal adhesion cascade , miR-29s inhibits cancers cell motility by regulating laminin-integrin signaling , --- , .

Techniques: Control, Migration, Biomarker Discovery, Activity Assay, Expressing

Journal: Frontiers in Pharmacology

Article Title: Pharmacological impact of microRNAs in head and neck squamous cell carcinoma: Prevailing insights on molecular pathways, diagnosis, and nanomedicine treatment

doi: 10.3389/fphar.2023.1174330

Figure Lengend Snippet: Summary of a variety of applications of nanomedicines in HNSCC regulation.

Article Snippet: miR-29s , HNSCC , Healthy tissues and entangled tumors , 23 patients , FaDU and SAS , ITGA6 and LAMC2 , Focal adhesion cascade , miR-29s inhibits cancers cell motility by regulating laminin-integrin signaling , --- , .

Techniques: Polymer, Histopathology, Homogenization, Permeability, Solubility, In Vivo, Expressing, Blocking Assay, Solvent, Evaporation, Inhibition, Formulation, Marker, Labeling, Activation Assay, Electroporation

Journal: Biomolecules

Article Title: Adaptation to Arginine Deprivation Leads to a More Aggressive, Therapy-Resistant Phenotype in HNSCC Cells

doi: 10.3390/biom15060900

Figure Lengend Snippet: SAS-R9 cells are relatively radioresistant. ( A ) Relative cell radiosensitivity was analyzed using a 2-D radiobiological colony forming assay; error bars = SD; ** p < 0.01. ( B ) Representative images of 2-D colonies from one out of four experiments. ( C ) Plating efficacy of sham-irradiated SAS and SAS-R9 cells. Error bars = SD. ( D ) DNA double-stranded breaks (DSBs) were analyzed by γ-H2A.X foci analysis in SAS and SAS-R9 cells 24 h after 4 Gy of X-ray irradiation; * p < 0.05. ( E ) Images show the exemplary SAS and SAS-R9 cells with γ-H2A.X foci24 h after 4 Gy of X-ray irradiation. Scale bars = 10 µm; n.s.—not significant. ( F ) The foci-negative populations of SAS and SAS-R9 cells 24 h after 4 Gy of X-ray irradiation. The foci-negative cells were defined as cells with ≤3 foci per nuclei. ( G ) The RT2 transcriptomic gene sets that are most significantly deregulated in SAS-R9 cells have a similar pattern of changes in FaDu RR cells; ** p < 0.01; *** p < 0.001. ( H ) Our findings suggest that cell selection and reprogramming, which are induced by repeated exposure to ADT, can result in tumor cell resistance to radiotherapy-induced DNA damage.

Article Snippet: Furthermore, a comparative analysis of the transcriptomic profiling in SAS-R9 and HNSCC FaDu RR, which were repeatedly irradiated with 12 fractions of 4 Gy of X-rays and acquired relative radioresistance [ ], revealed that the transcriptomic gene sets that are most significantly deregulated in SAS-R9 cells have a similar pattern of changes in FaDu RR cells ( G).

Techniques: Irradiation, Selection

Journal: Biomolecules

Article Title: Natural Compounds Modulate Drug Transporter Mediated Oral Cancer Treatment

doi: 10.3390/biom10091335

Figure Lengend Snippet: ATP-binding cassette G2 (ABCG2) expression was different in varied head and neck cancer (HNC) cell lines, which affected the amount of protoporphyrin IX (PpIX) and the survival percentage while the cells were treated with 5-aminolevulinic acid photodynamic therapy (ALA-PDT), and was modulated by different compounds. ( A ) Western blot analysis showed that ABCG2 was highly expressed in OECM1 but not in SAS, HSC3, and Fadu cells. ( B ) In parental status, PpIX amount of the four HNC cell lines showed no significant difference. For those cells treated with ALA, OECM1 produced the lowest amount of PpIX in comparison with SAS, HSC3, and Fadu cells. Switch of SAS cells from a medium containing 25 mM glucose to 5.5 mM glucose ( C ) induced ABCG2 expression and ( D ) downregulated PpIX accumulation in a time-dependent manner. ( E ) Western blot analysis for phospho-Akt, total Akt, Nrf2, and ABCG2 protein expression in OECM1 and SASL90d cells with/without treatment of PI3K inhibitor LY294002 ( n ≥ 3; * p < 0.05; *** p < 0.001). IC 50 of ALA-PDT treatment was measured in ABCG2-enriched cells: ( F ) OECM1, parental SAS, and SASL90d cells; ( G ) OECM1; and ( H ) SASL90d. ABCG2 level was significantly inhibited by gefitinib, epigallocatechin gallate (EGCG), and curcumin but not by naringenin. n ≥ 3, * p < 0.05, *** p < 0.001, **** p < 0.0001.

Article Snippet: HNC cell lines, including OECM1, SAS, HSC3, and FaDu, were obtained from the Japanese Collection of Research Bioresources (Tokyo, Japan).

Techniques: Binding Assay, Expressing, Western Blot, Produced, Comparison