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Image Search Results
Journal: Molecular Medicine
Article Title: Simvastatin and a Plant Galactolipid Protect Animals from Septic Shock by Regulating Oxylipin Mediator Dynamics through the MAPK-cPLA 2 Signaling Pathway
doi: 10.2119/molmed.2015.00082
Figure Lengend Snippet: Immunofluorescence staining of inflammatory markers in the liver, lungs and kidneys. Representative immunofluorescence staining images of (A) hepatic COX-2 expression and F4 / 80+ cell population in the sinusoids of the liver tissue, (B) HIF-1 expression and F4 / 80+ cell population in the renal glomeruli of the kidney tissue, and (C) PPAR-γ expression and Ly6G+ cell population in the lung alveoli (scale bars = 50 μmol/L). (D) Graphical representation of mean fluorescence intensities for proteins detected in each image were normalized against background intensities of the vehicle control group, expressed as arbitrary fluorescence units (A.U.) (N = 4, P < 0.05, ANOVA, post hoc LSD; values with different letters are significantly different).
Article Snippet: Antibodies used were as follows: MEK 1/2, phospho-MEK 1/2, cPLA 2 , phospho-cPLA 2 , p38, phospho-p38, IKK-α, phospho-IKK, phospho-JNK, phospho-ERK 1/2 (Cell Signaling); sEH, PPAR-γ, CYP4A (Abcam); COX-2 (Cayman); actin (Millipore); HIF-1α, JNK 1/2, ERK 1 (Santa Cruz); and COX-2 and
Techniques: Immunofluorescence, Staining, Expressing, Fluorescence