Journal: iScience

Article Title: Sensitization of FOLFOX-resistant colorectal cancer cells via the modulation of a novel pathway involving protein phosphatase 2A

doi: 10.1016/j.isci.2022.104518

Figure Lengend Snippet: NSC49L-mediated activation of PP2AC (A) chemical structure of NSC49L. (B) human rPP2AC activity determined with the malachite green assay. (C) K act(NSC49L) and V max values of NSC49L with PP2AC. (D) PP2A activity using immune complexes isolated with anti-PP2AC antibody from control and NSC49L-treated FOLFOX-HT29 cells. Data are the mean ± SE of three experiments. (E) Okadaic acid (OA), a PP2A inhibitor, blocks NSC49L and TRAIL-induced cytotoxicity to FOLFOX-HT29 cells. Cells were treated with different concentrations of NSC49L, TRAIL and OKA either alone are in combination as indicated in the figure. After 72 h treatment, cell viability was determined using the MTT-assay. ∗ = Significantly different as compared to NSC49L group, and ∗∗ = significantly different as compared to NSC49L+TRAIL group. (F) effect of OA on the IC 50 of the TRAIL. Data are the mean ± SE of four estimations. The p < 0.05 is considered significant.

Article Snippet: β-Actin (13E5) mAb (Rabbit) , Cell Signaling Technology , Cat# 4970; RRID:AB_2223172.

Techniques: Activation Assay, Activity Assay, Malachite Green Assay, Isolation, Control, MTT Assay

Journal: iScience

Article Title: Sensitization of FOLFOX-resistant colorectal cancer cells via the modulation of a novel pathway involving protein phosphatase 2A

doi: 10.1016/j.isci.2022.104518

Figure Lengend Snippet: NSC49L treatment increases the colocalization of eIF4E with unphosphorylated 4EBP1 and inhibits p21 protein synthesis in FOLFOX-HT29 cells (A) IHC analysis. FOLFOX-HT29 cells were treated with 20 μM of NSC49L for 24 h. After treatment, the colocalization of eIF4E and 4EBP1 was determined by IHC. Yellow color in the merge column shows the colocalization of these two proteins. Images were captured with a Nikon A1RMPsi-STORM4.0 confocal microscope (Melville, NY) at 60× magnification. (B) Global protein synthesis. FOLFOX HT29 cells were treated as above with 20 μM of NSC49L for 24 h. Cells were treated with 10 μg/mL of puromycin or puromycin plus 10 μg/mL of CHX for 15 min. Cells were washed and incubated in a fresh medium without drugs for an additional 2 h. Samples were blotted with anti-puromycin antibody. (C) Validate the experimental conditions, a concentration-dependent effect on p21 expression was performed. Cells were mock treated (control) or treated with 10 and 20 μM of NSC49L for 24 h and p21 level was determined. (D) Evaluate the effect of NSC49L on the regulation of p21 biosynthesis, we set up the experiment as in B. To determine the incorporation of puromycin into nascent p21, the pre-immunoprecipitated lysate was subjected to anti-puromycin immunoblot. (E) Quantitative analysis of the blot shown in panel D, which is presented as the ImageJ arbitrary units. Data are Mean ± SE of three experiments. The p-value is shown on the top of the graph. (F) β-actin was used as an internal control for equal loading. See also Figure S6 .

Article Snippet: β-Actin (13E5) mAb (Rabbit) , Cell Signaling Technology , Cat# 4970; RRID:AB_2223172.

Techniques: Microscopy, Incubation, Concentration Assay, Expressing, Control, Immunoprecipitation, Western Blot

Journal: iScience

Article Title: Sensitization of FOLFOX-resistant colorectal cancer cells via the modulation of a novel pathway involving protein phosphatase 2A

doi: 10.1016/j.isci.2022.104518

Figure Lengend Snippet:

Article Snippet: β-Actin (13E5) mAb (Rabbit) , Cell Signaling Technology , Cat# 4970; RRID:AB_2223172.

Techniques: Control, Recombinant, Purification, Bicinchoninic Acid Protein Assay, Membrane, Saline, Phospho-proteomics, Gene Expression, Software

Journal: Cell Reports Medicine

Article Title: Targeting pancreatic cancer glutamine dependency confers vulnerability to GPX4-dependent ferroptosis

doi: 10.1016/j.xcrm.2025.101928

Figure Lengend Snippet:

Article Snippet: HRP-conjugated Beta Actin , Proteintech , Cat# HRP-60008; RRID: AB_2819183.

Techniques: Virus, CRISPR, Recombinant, Protease Inhibitor, Lysis, Ligation, Reverse Transcription, SYBR Green Assay, Glo Assay, Sample Prep, DNA Extraction, Enzyme-linked Immunosorbent Assay, Multiple Displacement Amplification, Flow Cytometry, Software

Journal: Molecular cell

Article Title: Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells

doi: 10.1016/j.molcel.2018.07.020

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal Actin (C4) , Santa Cruz , RRID:AB_2714189; Cat# sc-47778.

Techniques: Magnetic Beads, Virus, Recombinant, Reverse Transcription, Protease Inhibitor, Plasmid Preparation, SYBR Green Assay, shRNA, Mutagenesis, Software

Journal: Cell Stem Cell

Article Title: Optimized human intestinal organoid model reveals interleukin-22-dependency of paneth cell formation

doi: 10.1016/j.stem.2022.08.002

Figure Lengend Snippet:

Article Snippet: β-Actin (13E5) Rabbit mAb (HRP Conjugate) , Cell Signaling Technology , Cat#5125S; RRID: AB_1903890.

Techniques: Recombinant, Reverse Transcription, SYBR Green Assay, Flow Cytometry, Software

Journal: Cell Reports

Article Title: Mutant FUS and ELAVL4 (HuD) Aberrant Crosstalk in Amyotrophic Lateral Sclerosis

doi: 10.1016/j.celrep.2019.05.085

Figure Lengend Snippet:

Article Snippet: Goat polyclonal anti-Actin antibody , Santa Cruz Biotechnology , Cat#sc-1616; RRID: AB_630836.

Techniques: Recombinant, Luciferase, Plasmid Preparation, SYBR Green Assay, Software, Imaging, Sequencing, Gene Expression, Expressing