Journal: Microbial Cell Factories

Article Title: Efficient production of bacterial antibiotics aminoriboflavin and roseoflavin in eukaryotic microorganisms, yeasts

doi: 10.1186/s12934-023-02129-8

Figure Lengend Snippet: Growth inhibition of Staphylococcus aureus (Sa) ATCC 25,923 and MRSA ATCC 43,300 (a) and Listeria monocytogenes (Lm) ATCC 19,113 (b) by 200 mg L -1 of aminoriboflavin (AF)

Article Snippet: 200 mg L -1 of AF showed similar bacteriostatic activity against S. aureus ATCC 25,923 and MRSA ATCC 43,300 (Fig. a).

Techniques: Inhibition

Journal: Stem Cells Translational Medicine

Article Title: Human cord blood‐derived regulatory T ‐cell therapy modulates the central and peripheral immune response after traumatic brain injury

doi: 10.1002/sctm.19-0444

Figure Lengend Snippet: Multicolor flow cytometry microglia/myeloid cell panel

Article Snippet: BV421 , P2Y12 , n/a , Alomone Labs , APR‐020‐F , Mediates microglia chemotaxis.

Techniques: Flow Cytometry, Marker, Chemotaxis Assay

Journal: Stem Cells Translational Medicine

Article Title: Human cord blood‐derived regulatory T ‐cell therapy modulates the central and peripheral immune response after traumatic brain injury

doi: 10.1002/sctm.19-0444

Figure Lengend Snippet: t‐SNE visualization of changes in microglia populations after CCI and treatment. A, Visualization of changes in cell clusters at 7 days post‐CCI (left) and at 30 days post‐CCI (right). At 7 days post‐CCI, there are clear differences between the sham and CCI plot density plots (top row); however, there are no clear differences between the CCI and Treg 24 hours plots. The antibody heat maps demonstrate that these CCI‐specific cell clusters are positive for CD45, CD11bc, and P2Y12, indicating that these cells are microglia. At 30 days post‐CCI, there are persistent visual differences between the sham and CCI density plots; furthermore, the antibody heat maps demonstrate that these cell clusters are also microglia. B, Detailed visualized of CCI‐specific cell clusters (black circles) at 30 days post‐CCI (top). Below, these cell clusters are visualized in more detail with the CD11bc antibody heat map. Within these clusters, there are clear differences between the sham and CCI plots, indicating that the CCI results in long term changes in microglia populations at 30 days after injury. Furthermore, there are clear differences between the CCI and Treg 24 hours groups, with fewer cells present in the treatment group. Replicates: 7d cohort, N = 8; 30d cohort, N = 6. CCI, controlled cortical impact; Treg, regulatory T cells; t‐SNE, t‐distributed stochastic neighbor embedding

Article Snippet: BV421 , P2Y12 , n/a , Alomone Labs , APR‐020‐F , Mediates microglia chemotaxis.

Techniques:

Journal: Stem Cells Translational Medicine

Article Title: Human cord blood‐derived regulatory T ‐cell therapy modulates the central and peripheral immune response after traumatic brain injury

doi: 10.1002/sctm.19-0444

Figure Lengend Snippet: Flow cytometric characterization and comparison of microglia in the contralateral (uninjured) and ipsilateral (injured) hemispheres after CCI and Treg therapy at 7 days post‐CCI and 30 days post‐CCI. A, Absolute microglia counts, measured as cells/mg of brain tissue, at 7 days post‐CCI. B‐D, MFI of CD45, CD11bc, and P2Y12 expression 7 days post‐CCI, respectively. E, Absolute microglia counts, measured as cells/mg of brain tissue, at 30 days post‐CCI. F‐H, MFI of CD45, CD11bc, and P2Y12 expression 30 days post‐CCI, respectively. Statistical significance between sham and CCI is indicated with # P ≤ .05, ## P ≤ .01, ### P ≤ .001, and #### P ≤ .0001. Replicates: 7d cohort, N = 8; 30d cohort, N = 6. Statistical significance between CCI and Treg is indicated with * P ≤ .05, ** P ≤ .01, *** P ≤ .001, and **** P ≤ 0.0001. CCI, controlled cortical impact; MFI, median fluorescent intensity; Treg, regulatory T cells

Article Snippet: BV421 , P2Y12 , n/a , Alomone Labs , APR‐020‐F , Mediates microglia chemotaxis.

Techniques: Expressing

Journal: Chemical & Biomedical Imaging

Article Title: Recombinant–Chemosynthetic Biosensors for Probing Cell Surface Signaling of Red Blood Cells and Other Cells

doi: 10.1021/cbmi.4c00067

Figure Lengend Snippet: Figure 2. (A) Optimization of the cell labeling protocol in HeLa cells was performed under three different conditions: (left) cells are in MEM/ DFBS/PS containing HeLa media and purified sortase A and GCaMP6s-LPDTG proteins are in 1% PBS; (middle) cells and purified sortase A and GCaMP6s-LPDTG proteins are in 1% PBS; (right) cells are in 1% PBS and purified sortase A and GCaMP6s-LPDTG proteins are in elution buffer. (B) HeLa cells labeled with ExtraCal showed a reduction in ExtraCal fluorescence upon EDTA addition and its fluorescence intensity was increased upon addition of HBSS containing Ca2+ (n = 25 cells from 3 repeats). The plot shows fluorescence change of ExtraCal upon EDTA followed by HBSS-With- Ca2+ addition over the course of 6 min. The error bars represent SD (standard error of standard deviation). Scale bar = 5 μm. EDTA, ethylenediaminetetraacetic acid; HBSS, Hanks’ balanced salt solution.

Article Snippet: EDTA Disodium salt dihydrate (Fisher Scientific), HBSS-With-Ca2+ (Gibco), Ca2+ free HBSS (Gibco), ATP (Chem-Impex International, Inc.)), Rhod-2 AM (Cayman Chemical), YM-254890 (Cayman Chemical), oxyrase (Oxyrase, Inc.), MgCl2 hexahydrate (SigmaAldrich), PMSF (Research Products International), Lysozyme (Gold Biotechnology), Luciferin (Gold Biotechnology), and EZ-Run Prestained Rec Protein Ladder (Fisher Scientific).

Techniques: Labeling, Purification, Fluorescence, Standard Deviation

Journal: Bioconjugate chemistry

Article Title: A Trimethoprim Conjugate of Thiomaltose Has Enhanced Antibacterial Efficacy In Vivo

doi: 10.1021/acs.bioconjchem.8b00177

Figure Lengend Snippet: (A) Thiomaltose trimethoprim (TM-TMP): A new trimethoprim prodrug with enhanced water solubility that targets bacteria. (B) Proposed cleavage of TM-TMP in the presence of exogenous thiols.

Article Snippet: Control groups included mice untreated with any drug, and mice treated with trimethoprim (Chem-Impex International Inc. Wood Dale, IL), while the experimental group was treated with thiomaltose-trimethoprim (TM-TMP).

Techniques: Solubility, Bacteria

Journal: Bioconjugate chemistry

Article Title: A Trimethoprim Conjugate of Thiomaltose Has Enhanced Antibacterial Efficacy In Vivo

doi: 10.1021/acs.bioconjchem.8b00177

Figure Lengend Snippet: Synthesis of thiomaltose-trimethoprim (TM-TMP). (a) DCM, TEA, rt, 55%. (b) DCC, DMAP, TEA, DMF, rt, 29%. (c) CuI, DIPEA, DMSO, rt, 65%.

Article Snippet: Control groups included mice untreated with any drug, and mice treated with trimethoprim (Chem-Impex International Inc. Wood Dale, IL), while the experimental group was treated with thiomaltose-trimethoprim (TM-TMP).

Techniques: