Journal: Plant Signaling & Behavior

Article Title: A sweet cycle for Arabidopsis G-proteins

doi:

Figure Lengend Snippet: Effect of mutations in the β2/β3 loop, α5 helix, and the β6/α5 loop on the GDP release rate of Gαi1. The GDP release rate of wild type and mutant Gαi1 subunit was measured using [35S]GTPγS radioligand binding, as described.78 GTPγS binding is an accurate method of measuring GDP release, given that GDP release is the rate limiting step in the nucleotide exchange process.11 Data were fit to a single exponential function using GraphPad PRISM 3.0. Observed rate constants: (A) wild type, 0.036 min−1; K192A, 0.056 min−1; F336A, 0.1844 min−1 (B) wild type, 0.068 min−1; 0.16 min−1, A326S. Note: the rate enhancement engendered by the A326S mutation in this experiment was only 3-fold, not the 20-fold previously reported.32 We observed faster GDP release by A326S Gαi1 in some experiments. We hypothesize that this may be due to the idiosyncratic effects of polyoxyethylene 10-lauryl ether (lubrol) on GDP release, as described.12

Article Snippet: 11 Data were fit to a single exponential function using GraphPad PRISM 3.0.

Techniques: Mutagenesis, Binding Assay

Journal: Plant Signaling & Behavior

Article Title: A sweet cycle for Arabidopsis G-proteins

doi:

Figure Lengend Snippet: Saturation binding analysis of the affinity of Mg2+·GTPγS for AtGPA1. 1 nM AtGPA1 was mixed with various concentrations of [35S] GTPγS in the presence of 25 mM MgCl2.6,78 Bound GTPγS was quantified by filtration and liquid scintillation as described.6,78 Non-specific binding was determined in the presence of 100 mM unlabeled GTPγS. Specific binding was fit to a saturation binding isotherm (Y = Bmax + X / (KD + X)) using GraphPad PRISM 3.0.

Article Snippet: 11 Data were fit to a single exponential function using GraphPad PRISM 3.0.

Techniques: Binding Assay, Filtration

Journal: PLoS ONE

Article Title: Natural regeneration on seismic lines influences movement behaviour of wolves and grizzly bears

doi: 10.1371/journal.pone.0195480

Figure Lengend Snippet: Relative probability of step selection by wolves in west-central Alberta between 2003 and 2009 during the denning season in relation to distance to seismic lines (represented as an exponential decay (1-exp -0.002* Distance (m) )), seismic line vegetation height (visualised using the mean of the lower (Low), middle (Mod), and upper (High) quantiles), and landcover intersecting seismic lines (Non-forest, Mixed, Conifer). Shaded areas are 95% confidence intervals around relative predicted probabilities of step selection.

Article Snippet: For step-selection function analyses, to represent the diminishing effect of seismic lines and attributes of seismic lines on movement behaviour with increasing distance to seismic lines, we used an exponential decay function as the measure of distance from the end of each step to each seismic line segment ( eDist ; 1-exp -0.002*Distance(m) ; ).

Techniques: Selection

Journal: PLoS ONE

Article Title: Natural regeneration on seismic lines influences movement behaviour of wolves and grizzly bears

doi: 10.1371/journal.pone.0195480

Figure Lengend Snippet: Relative probability of step selection by wolves in west-central Alberta between 2003 and 2009 during the rendezvous season in relation to distance to seismic lines (represented as an exponential decay (1-exp -0.002* Distance (m) )) and seismic line vegetation height (visualised using the mean of the lower (Low), middle (Mod), and upper (High) quantiles). Seismic line wetness ( eWAM ) was held at the mean for prediction. Shaded areas are 95% confidence intervals around relative predicted probabilities of step selection.

Article Snippet: For step-selection function analyses, to represent the diminishing effect of seismic lines and attributes of seismic lines on movement behaviour with increasing distance to seismic lines, we used an exponential decay function as the measure of distance from the end of each step to each seismic line segment ( eDist ; 1-exp -0.002*Distance(m) ; ).

Techniques: Selection

Journal: PLoS ONE

Article Title: Natural regeneration on seismic lines influences movement behaviour of wolves and grizzly bears

doi: 10.1371/journal.pone.0195480

Figure Lengend Snippet: Relative probability of step selection by grizzly bears in west-central Alberta between 2005 and 2009 during spring in relation to distance to seismic lines (represented as an exponential decay (1-exp -0.002* Distance (m) )), seismic line vegetation height (visualised using the mean of the lower (Low), middle (Mod), and upper (High) quantiles), and landcover intersecting seismic lines (Non-forest, Mixed, Conifer). Shaded areas are 95% confidence intervals around relative predicted probabilities of step selection.

Article Snippet: For step-selection function analyses, to represent the diminishing effect of seismic lines and attributes of seismic lines on movement behaviour with increasing distance to seismic lines, we used an exponential decay function as the measure of distance from the end of each step to each seismic line segment ( eDist ; 1-exp -0.002*Distance(m) ; ).

Techniques: Selection

Journal: PLoS ONE

Article Title: Natural regeneration on seismic lines influences movement behaviour of wolves and grizzly bears

doi: 10.1371/journal.pone.0195480

Figure Lengend Snippet: Relative probability of step selection by male grizzly bears in west-central Alberta between 2005 and 2009 during spring in relation to distance to seismic lines (represented as an exponential decay (1-exp -0.002* Distance (m) )), seismic line vegetation height (visualised using the mean of the lower (Low), middle (Mod), and upper (High) quantiles), and seismic lines wetness (represented as an exponential decay (1-exp-1.55*WAM(m)) and visualised using the mean of the lower (Wet), middle (Mesic), and upper (Dry) quantiles. Shaded areas are 95% confidence intervals around relative predicted probabilities of step selection.

Article Snippet: For step-selection function analyses, to represent the diminishing effect of seismic lines and attributes of seismic lines on movement behaviour with increasing distance to seismic lines, we used an exponential decay function as the measure of distance from the end of each step to each seismic line segment ( eDist ; 1-exp -0.002*Distance(m) ; ).

Techniques: Selection

Journal: PLoS ONE

Article Title: Natural regeneration on seismic lines influences movement behaviour of wolves and grizzly bears

doi: 10.1371/journal.pone.0195480

Figure Lengend Snippet: Relative probability of step selection by female grizzly bears in west-central Alberta between 2005 and 2009 during summer and fall in relation to distance to seismic lines (represented as an exponential decay (1-exp -0.002* Distance (m) )), seismic line vegetation height (visualised using the mean of the lower (Low), middle (Mod), and upper (High) quantiles), and landcover intersecting seismic lines (Non-forest, Mixed, Conifer). Shaded areas are 95% confidence intervals around relative predicted probabilities of step selection.

Article Snippet: For step-selection function analyses, to represent the diminishing effect of seismic lines and attributes of seismic lines on movement behaviour with increasing distance to seismic lines, we used an exponential decay function as the measure of distance from the end of each step to each seismic line segment ( eDist ; 1-exp -0.002*Distance(m) ; ).

Techniques: Selection

Journal: PLoS ONE

Article Title: Natural regeneration on seismic lines influences movement behaviour of wolves and grizzly bears

doi: 10.1371/journal.pone.0195480

Figure Lengend Snippet: Relative probability of step selection by male grizzly bears in west-central Alberta between 2005 and 2009 during fall in relation to distance to seismic lines (represented as an exponential decay (1-exp -0.002* Distance (m) )) and seismic line vegetation height (visualised using the mean of the lower (Low), middle (Mod), and upper (High) quantiles). Results are shown for the reference category of landcover intersecting seismic lines (Non-forest). Shaded areas are 95% confidence intervals around relative predicted probabilities of step selection.

Article Snippet: For step-selection function analyses, to represent the diminishing effect of seismic lines and attributes of seismic lines on movement behaviour with increasing distance to seismic lines, we used an exponential decay function as the measure of distance from the end of each step to each seismic line segment ( eDist ; 1-exp -0.002*Distance(m) ; ).

Techniques: Selection