Journal:

Article Title: Ionic selectivity of native ATP-activated (P2X) receptor channels in dissociated neurones from rat parasympathetic ganglia

doi: 10.1111/j.1469-7793.2001.00423.x

Figure Lengend Snippet: A, superimposed ATP-evoked currents obtained at a holding potential of −80 mV immediately after whole-cell recording was established (control, thick trace) and approximately 15 min after dialysis with pipette intracellular solutions containing anti-P2X1, anti-P2X2, anti-P2X4, anti-P2X2+ anti-P2X4 and heat-inactivated anti-P2X2+ anti-P2X4 antibodies (thin trace). Antibodies diluted 1:200. B, bar graph of relative peak current amplitude obtained following intracellular dialysis with anti-P2X1 (n = 7), anti-P2X2 (n = 5), anti-P2X4 (n = 5), anti-P2X2+ anti-P2X4 (n = 6) and heat-inactivated anti-P2X2+ anti-P2X4 (n = 5) antibodies expressed relative to that obtained in the absence of anti-P2X antibodies (*P < 0.05, **P < 0.005). Data were normalized with respect to those obtained immediately upon establishing the whole-cell recording configuration.

Article Snippet: Anti-P2X antibodies specific for the P2X receptor subtypes were supplied by Alomone Laboratories (Jerusalem, Israel).

Techniques: Transferring