Journal: Journal of Neuroinflammation

Article Title: Edaravone ameliorates depressive and anxiety-like behaviors via Sirt1/Nrf2/HO-1/Gpx4 pathway

doi: 10.1186/s12974-022-02400-6

Figure Lengend Snippet: Blockade of Sirt1 abolished the antidepressant and anxiolytic effects of EDA. A Schematic timeline of the experimental procedure. B Social interaction test. C Sucrose preference test. D Open field test. E Elevated plus maze test. F Tail suspension test. G Forced swimming test. H Novel object recognition test. All the data are expressed as mean ± SEM ( n = 8 per group). ** p < 0.01, *** p < 0.001 versus the CON group. # p < 0.05, ## p < 0.01, ### p < 0.001 versus the CSDS group. & p < 0.05 versus the CSDS + EDA group

Article Snippet: EX527 (a Sirt1 inhibitor) and ML385 (a Nrf2 inhibitor) were obtained from MedChemExpress (New Jersey, USA).

Techniques: Suspension

Journal: Journal of Neuroinflammation

Article Title: Edaravone ameliorates depressive and anxiety-like behaviors via Sirt1/Nrf2/HO-1/Gpx4 pathway

doi: 10.1186/s12974-022-02400-6

Figure Lengend Snippet: Primer sequences used in the qRT-PCR

Article Snippet: EX527 (a Sirt1 inhibitor) and ML385 (a Nrf2 inhibitor) were obtained from MedChemExpress (New Jersey, USA).

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Edaravone ameliorates depressive and anxiety-like behaviors via Sirt1/Nrf2/HO-1/Gpx4 pathway

doi: 10.1186/s12974-022-02400-6

Figure Lengend Snippet: Effect of EDA on Sirt1/Nrf2/HO-1/Gpx4 and TLR4/NF-κB signaling pathways. A Representative WB bands in the hippocampal region. B – G Statistical graphs of relative protein expression of Sirt1 ( B ), Nrf2 ( C ), HO-1 ( D ), Gpx4 ( E ), TLR4 ( F ), and p-NF-κB ( G ). H Representative WB bands in the mPFC region. I – N Statistical graphs of relative protein expression of Sirt1 ( I ), Nrf2 ( J ), HO-1 ( K ), Gpx4 ( L ), TLR4 ( M ), and p-NF-κB ( N ). Data are presented as mean ± SEM ( n = 4 per group). * p < 0.05, ** p < 0.01, ***p < 0.001 versus the CON + Vehicle group. # p < 0.05, ## p < 0.01, ### p < 0.001 versus the CSDS + Vehicle group

Article Snippet: EX527 (a Sirt1 inhibitor) and ML385 (a Nrf2 inhibitor) were obtained from MedChemExpress (New Jersey, USA).

Techniques: Expressing

Journal: Journal of Neuroinflammation

Article Title: Edaravone ameliorates depressive and anxiety-like behaviors via Sirt1/Nrf2/HO-1/Gpx4 pathway

doi: 10.1186/s12974-022-02400-6

Figure Lengend Snippet: Impact of Sirt1 inhibitor on EDA-induced Sirt1/Nrf2/HO-1/Gpx4 signaling pathway in the Hip of CSDS mice. A Representative WB bands. B – E Protein levels of Sirt1 ( B ), Nrf2 ( c ), HO-1 ( D ), and Gpx4 ( E ) in the Hip. Data are presented as mean ± SEM ( n = 3 per group). * p < 0.05, ** p < 0.01, *** p < 0.001 versus the CON group. ## p < 0.01, ### p < 0.001 versus the CSDS group. && p < 0.01, &&& p < 0.001 versus the CSDS + EDA group

Article Snippet: EX527 (a Sirt1 inhibitor) and ML385 (a Nrf2 inhibitor) were obtained from MedChemExpress (New Jersey, USA).

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Edaravone ameliorates depressive and anxiety-like behaviors via Sirt1/Nrf2/HO-1/Gpx4 pathway

doi: 10.1186/s12974-022-02400-6

Figure Lengend Snippet: Impact of Nrf2 inhibitor on EDA-induced Sirt1/Nrf2/HO-1/Gpx4 signaling pathway in the Hip of CSDS mice. A Representative WB bands. B – E Protein levels of Sirt1 ( B ), Nrf2 ( C ), HO-1 ( D ), and Gpx4 ( E ) in the Hip. Data are presented as mean ± SEM ( n = 3 per group). * p < 0.05, ** p < 0.01, *** p < 0.001 versus the CON group. # p < 0.05, ### p < 0.001 versus the CSDS group. && p < 0.01, &&& p < 0.001 versus the CSDS + EDA group

Article Snippet: EX527 (a Sirt1 inhibitor) and ML385 (a Nrf2 inhibitor) were obtained from MedChemExpress (New Jersey, USA).

Techniques:

Journal: PLoS ONE

Article Title: Synergistic Interactions between HDAC and Sirtuin Inhibitors in Human Leukemia Cells

doi: 10.1371/journal.pone.0022739

Figure Lengend Snippet: Primary AML cells were incubated with or without sirtuin inhibitors (EX527, sirtinol, cambinol) in the presence or absence of VA at the indicated concentrations. Viability was assessed 48 h later by PI cell staining and flow cytometry. CI values refer to the highest drug concentrations used. CI CT s for each drug combination are presented in the lower insets. An effect of 1 corresponds to 100% specific death whereas an effect of 0 corresponds to 0% specific death.

Article Snippet: EX527 was from Tocris Bioscience (Bristol, UK).

Techniques: Incubation, Staining, Flow Cytometry

Journal: PLoS ONE

Article Title: Synergistic Interactions between HDAC and Sirtuin Inhibitors in Human Leukemia Cells

doi: 10.1371/journal.pone.0022739

Figure Lengend Snippet: A, 3×10 6 primary AML cells/well were plated in 6-well plates and incubated in the presence or absence of 50 µM cambinol, 100 µg/ml VA, or their combination. ΔΨ m was monitored at the indicated time points by TMRE staining and flow cytometry. B, 1×10 6 Jurkat cells were plated in 6-well plates and incubated for 48 h in the presence or absence of 100 µg/ml VA. Thereafter, intracellular Bax content was determined by flow cytometry. C, D, Jurkat cells were transduced with either pMIG or pMIG-Bax. Infected cells were FACS sorted, allowed to expand, and subsequently used for flow cytometric detection of intracellular Bax (C) and for viability assays (D). For these, pMIG- or pMIG-Bax-transduced Jurkat were plated in 96-well plates and incubated in the presence or absence of EX527 or cambinol at the indicated concentrations. Viability was determined by PI staining and flow cytometry 48 h later. A–C, one representative experiment out of three is presented. D, Results are means ± SD of three separate experiments.

Article Snippet: EX527 was from Tocris Bioscience (Bristol, UK).

Techniques: Incubation, Staining, Flow Cytometry, Transduction, Infection

Journal: PLoS ONE

Article Title: Synergistic Interactions between HDAC and Sirtuin Inhibitors in Human Leukemia Cells

doi: 10.1371/journal.pone.0022739

Figure Lengend Snippet: A, B, U937 and 697 cells were transduced to either express an anti-EGFP shRNA (EGFP-sh) or a validated anti-Bax shRNA (Bax-sh). Thereafter cells were used for cell lysate preparation and Bax and γ-tubulin were detected by immunoblotting. B, EGFP-sh or Bax-sh 697 cells were incubated with or without 100 µg/ml VA, 75 µM EX527, 50 µM cambinol, or their combinations. Viability was assessed 36 h later by PI staining and flow cytometry. C, D, EGFP-sh or Bax-sh U937 cells were incubated with or without 100 µg/ml VA, 150 µM EX527, 100 µM cambinol, or their combinations. 36 h later, cells were imaged by light microscopy (D) and cell death was determined by flow cytometric quantification of PI-positive cells (C). A, D, one representative experiment out of three is presented. B, C, Results are means ± SD of three separate experiments. *: p<0.05.

Article Snippet: EX527 was from Tocris Bioscience (Bristol, UK).

Techniques: shRNA, Western Blot, Incubation, Staining, Flow Cytometry, Light Microscopy

Journal: International Journal of Molecular Sciences

Article Title: COX-2/sEH Dual Inhibitor Alleviates Hepatocyte Senescence in NAFLD Mice by Restoring Autophagy through Sirt1/PI3K/AKT/mTOR

doi: 10.3390/ijms23158267

Figure Lengend Snippet: PTUPB enhances hepatocyte autophagy by promoting Sirt1 expression. The protein expressions of Sirt1 in the liver (( A , B ), n = 6) and AML12 cells (( C , D ), n = 3) were measured by Western blot and quantitatively analyzed. Cells were treated with PTUPB (1 μM) or EX527 (10 μM) for 1 h before treatment with PA (200 µM) for 48 h. The protein expressions of Sirt1, γH2AX, Fundc1, and LC3II/I in AML12 cells were measured by Western blot and quantitatively analyzed (( E – I ), n = 3). Data are expressed as the mean ± SD. Differences among multiple groups were performed using ANOVA. Tukey’s test was used as a post hoc test for pairwise comparisons. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: An autophagy inhibitor 3-MA (5 mM, MCE, Dallas, TX, USA) or Sirt1 inhibitor EX527 (10 μM, Topscience, Shandong, China) was used to clarify the role of autophagy or Sirt1 in the anti-senescence effects of PTUPB in AML12 cells.

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: COX-2/sEH Dual Inhibitor Alleviates Hepatocyte Senescence in NAFLD Mice by Restoring Autophagy through Sirt1/PI3K/AKT/mTOR

doi: 10.3390/ijms23158267

Figure Lengend Snippet: PTUPB inhibits the PI3K/AKT/mTOR signaling pathway by promoting Sirt1 expression. The mTOR protein and its phosphorylation level in the liver were measured by Western blot and quantitatively analyzed (( A – C ), n = 6). Cells were treated with PTUPB (1 μM) for 1 h before treatment with PA (200 µM). The levels of p-mTOR, p-AKT, and p-PI3K in AML12 cells were measured by Western blot and quantitatively analyzed after PA stimulation for 30 min (( D – G ), n = 3). Cells were treated with PTUPB (1 μM) or EX527 (10 μM) for 1 h before treatment with PA (200 µM). The levels of p-mTOR, p-AKT, and p-PI3K in AML12 cells were measured by Western blot and quantitatively analyzed after PA stimulation for 30 min (( H – K ), n = 3). Data are expressed as the mean ± SD. Differences among multiple groups were performed using ANOVA. Tukey’s test was used as a post hoc test for pairwise comparisons. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: An autophagy inhibitor 3-MA (5 mM, MCE, Dallas, TX, USA) or Sirt1 inhibitor EX527 (10 μM, Topscience, Shandong, China) was used to clarify the role of autophagy or Sirt1 in the anti-senescence effects of PTUPB in AML12 cells.

Techniques: Expressing, Phospho-proteomics, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: COX-2/sEH Dual Inhibitor Alleviates Hepatocyte Senescence in NAFLD Mice by Restoring Autophagy through Sirt1/PI3K/AKT/mTOR

doi: 10.3390/ijms23158267

Figure Lengend Snippet: Antibodies were used in this study.

Article Snippet: An autophagy inhibitor 3-MA (5 mM, MCE, Dallas, TX, USA) or Sirt1 inhibitor EX527 (10 μM, Topscience, Shandong, China) was used to clarify the role of autophagy or Sirt1 in the anti-senescence effects of PTUPB in AML12 cells.

Techniques:

Journal: Scientific Reports

Article Title: Marein from Coreopsis tinctoria Nutt. alleviates oxidative stress and lipid accumulation via SIRT1/Nrf2 signaling

doi: 10.1038/s41598-025-97964-7

Figure Lengend Snippet: Marein restored SIRT1/Nrf2 signaling in H 2 O 2 -induced HepG2 cells. HepG2 cells were exposed 500μM H 2 O 2 condition for 24h, and then administrated by 5 μM Marein for another 24h. ( a , b ) mRNA expression levels of SIRT1 ( a ) and Nrf2 ( b ) were assessed using RT-qPCR. ( c ) Western blot analysis of SIRT1 and Nrf2 protein levels. ( d ) Immunofluorescence staining of Nrf2 nuclear localization (scale bar = 25 μm). Data are represented as mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Besides, HepG2 cells was incubated with 5 μM Marein (≥ 96% purity, Shanghai Yuanye Bio-Technology Co., Ltd., China) for 24 h . For inhibitor experiments, cells were pre-treated with SIRT1 inhibitor EX-527 (100 nM, #A10377, AdooQ BioScience, Nanjing, China) for 2 h before Marein treatment.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Staining

Journal: Scientific Reports

Article Title: Marein from Coreopsis tinctoria Nutt. alleviates oxidative stress and lipid accumulation via SIRT1/Nrf2 signaling

doi: 10.1038/s41598-025-97964-7

Figure Lengend Snippet: Inactivation of SIRT1/Nrf2 reversed the antioxidant ability of marein in H 2 O 2 -induced HepG2 cells. HepG2 cells were subjected to different groups: control, H 2 O 2 , H 2 O 2 + Marein, H 2 O 2 + Marein + EX-527 (SIRT1 inhibitor). ( a ) CCK-8 detection was used to examine cell viability. ( b ) LDH content was detected to examine oxidative stress injury. ( c – e ) MDA ( c ), SOD ( d ), and GSH-Px ( e ) levels were detected by ELISA. ( f ) DCFDA detected ROS level. Data are represented as mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Besides, HepG2 cells was incubated with 5 μM Marein (≥ 96% purity, Shanghai Yuanye Bio-Technology Co., Ltd., China) for 24 h . For inhibitor experiments, cells were pre-treated with SIRT1 inhibitor EX-527 (100 nM, #A10377, AdooQ BioScience, Nanjing, China) for 2 h before Marein treatment.

Techniques: Control, CCK-8 Assay, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Marein from Coreopsis tinctoria Nutt. alleviates oxidative stress and lipid accumulation via SIRT1/Nrf2 signaling

doi: 10.1038/s41598-025-97964-7

Figure Lengend Snippet: Inactivation of SIRT1/Nrf2 restrained protective role of marein in H 2 O 2 -triggered lipid accumulation. HepG2 cells were subjected to different groups: control, H 2 O 2 , H 2 O 2 + Marein, H 2 O 2 + Marein + EX-527 (SIRT1 inhibitor). ( a – d ) Determination of TC ( a ), TG ( b ), LDL-C ( c ), and HDL-C ( d ) levels by ELISA. ( e , f ) Detection of lipid metabolism related genes HMGCR ( e ) and LDLR ( f ) by RT-qPCR. ( g ) Western blot analysis of HMGCR and LDLR protein levels. Values were expressed as mean ± SD of three separate determinations. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Besides, HepG2 cells was incubated with 5 μM Marein (≥ 96% purity, Shanghai Yuanye Bio-Technology Co., Ltd., China) for 24 h . For inhibitor experiments, cells were pre-treated with SIRT1 inhibitor EX-527 (100 nM, #A10377, AdooQ BioScience, Nanjing, China) for 2 h before Marein treatment.

Techniques: Control, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot