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Primer Sequences Used for Quantitative Real-Time PCR

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Image Search Results

Journal: Endocrinology

Article Title: Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues

doi: 10.1210/en.2016-1607

Figure Lengend Snippet: Primer Sequences Used for Quantitative Real-Time PCR

Article Snippet: After the treatment with enhanced chemiluminescence reagent (Pierce; Thermo Scientific), the membranes were exposed to an X-ray film (Kodak; Sigma Aldrich, St. Louis, MO) and Image by Image Quant (GE Lifescience, Pittsburgh, PA) software for densitometric analysis ( 28, 32 ). table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Peptide/Protein Target Name of Antibody Manufacturer, Catalog Number Antibody RRID Species Dilution Used UCP1 Uncoupling protein1 antibody Abcam ab10983 AB_2241462 Rabbit polyclonal 1:1000 UCP2 Uncoupling protein2 antibody Novus Biologicals NB100-78377 AB_1085929 Rabbit polyclonal 1:1000 UCP3 Uncoupling protein3 antibody Abcam ab3477 AB_2304253 Rabbit polyclonal 1:1000 PRDM16 PRDM16 antibody (N16) Santa Cruz Biotech sc-130243 AB_2284344 Rabbit polyclonal 1:500 PGC-1 α PGC-1 α antibody (H-300) Santa Cruz Biotech sc-13067 AB_2166218 Rabbit polyclonal 1:1000 Adiponectin Adiponectin antibody R & D Systems MAB1119 AB_2305045 Rat monoclonal 1:1000 AMPK α AMPK α antibody Cell Signaling 2532 AB_330331 Rabbit monoclonal 1:1000 pAMPK α pAMPK α antibody Cell Signaling 2535 AB_331250 Rabbit monoclonal 1:1000 Glut4 Glucose transporter 4 antibody Abcam ab65267 AB_1140009 Mouse monoclonal 1:1000 p38MAPK p38MAPK antibody Cell Signaling 9212S AB_330713 Rabbit polyclonal 1:1000 pp38MAPK pp38MAPK antibody Cell Signaling 9211S AB_331641 Rabbit polyclonal 1:1000 ERK1/2 ERK1/2 antibody Cell Signaling 9102S AB_330744 Rabbit polyclonal 1:1000 pERK1/2 pERK1/2 antibody Cell Signaling 9101S AB_331646 Rabbit polyclonal 1:1000 Myf5 Myf5 antibody Santa Cruz Biotech sc-302 AB_631994 Rabbit polyclonal 1:1000 Cd137 Cd137 antibody Abcam ab3169 AB_303572 Mouse monoclonal 1:1000 BMP7 BMP7 antibody Abcam ab54904 AB_940598 Mouse monoclonal 1:1000 Eva1 Eva1 antibody Novus Biologicals NBP1-88937 AB_11016118 Rabbit polyclonal 1:1000 β -actin Beta actin antibody Santa Cruz Biotech sc-81178 AB_2223230 Mouse monoclonal 1:5000 GAPDH GAPDH antibody Millipore MAB374 AB_2107445 Mouse monoclonal 1:5000 pSmad2/3 pSmad2/3 antibody Cell Signaling 8828 AB_2631089 Rabbit monoclonal 1:1000 AcvRIIB AcvRIIB antibody Abcam ab76940 AB_1565807 Mouse monoclonal 1:1000 Smad3 Smad3 antibody Cell Signaling 9513 AB_2286450 Rabbit polyclonal 1:1000 Open in a separate window List of Antibodies

Techniques:

Journal: Endocrinology

Article Title: Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues

doi: 10.1210/en.2016-1607

Figure Lengend Snippet: List of Antibodies

Techniques:

Upregulation of beige/brown adipose specific markers in 3T3-L1 and BAT cells after follistatin treatment. (a) 3T3-L1 cells were allowed to differentiate under modified adipogenic differentiation condition for 6 days either in absence or presence of recombinant Fst protein (0.5 µg/mL) as described in materials and methods. Protein expression of CD137 and UCP1 was analyzed by western blot analysis and a representative data are shown. (b) Densitometric analysis showing relative increase in UCP1 and CD137 protein levels (n = 3). **P ≤ 0.01. (c) Mouse BAT cells were allowed to differentiate under adipogenic differentiation conditions 6 days either in absence or presence of recombinant Fst protein (0.5 µg/mL) as described in materials and methods. Protein expression of UCP1, Eva1, and Myf5 was analyzed by western blot analysis and a representative data are shown. (d) Densitometric analysis showing relative increase in UCP1, Eva1, and Myf5 protein levels following Fst treatment (n = 3). *P ≤ 0.05; **P ≤ 0.01. (e) Quantitative gene expression analysis of key beige markers in differentiating 3T3-L1 cells treated with or without recombinant Fst (n = 3). *P ≤ 0.05; **P ≤ 0.01. (f) Quantitative gene expression analysis of key BAT markers in differentiating BAT cells treated with or without recombinant Fst (n = 3). *P ≤ 0.05; **P ≤ 0.01.

Journal: Endocrinology

Article Title: Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues

doi: 10.1210/en.2016-1607

Figure Lengend Snippet: Upregulation of beige/brown adipose specific markers in 3T3-L1 and BAT cells after follistatin treatment. (a) 3T3-L1 cells were allowed to differentiate under modified adipogenic differentiation condition for 6 days either in absence or presence of recombinant Fst protein (0.5 µg/mL) as described in materials and methods. Protein expression of CD137 and UCP1 was analyzed by western blot analysis and a representative data are shown. (b) Densitometric analysis showing relative increase in UCP1 and CD137 protein levels (n = 3). **P ≤ 0.01. (c) Mouse BAT cells were allowed to differentiate under adipogenic differentiation conditions 6 days either in absence or presence of recombinant Fst protein (0.5 µg/mL) as described in materials and methods. Protein expression of UCP1, Eva1, and Myf5 was analyzed by western blot analysis and a representative data are shown. (d) Densitometric analysis showing relative increase in UCP1, Eva1, and Myf5 protein levels following Fst treatment (n = 3). *P ≤ 0.05; **P ≤ 0.01. (e) Quantitative gene expression analysis of key beige markers in differentiating 3T3-L1 cells treated with or without recombinant Fst (n = 3). *P ≤ 0.05; **P ≤ 0.01. (f) Quantitative gene expression analysis of key BAT markers in differentiating BAT cells treated with or without recombinant Fst (n = 3). *P ≤ 0.05; **P ≤ 0.01.

Techniques: Modification, Recombinant, Expressing, Western Blot, Gene Expression

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