Journal: Redox Biology

Article Title: The BRD7-P53-SLC25A28 axis regulates ferroptosis in hepatic stellate cells

doi: 10.1016/j.redox.2020.101619

Figure Lengend Snippet: Upregulated BRD7 protein level contributed to HSC ferroptosis. (A) The modified inducible CRISPR/Cas9 system was used to establish BRD7 knockout HSC lines, and the specific BRD7 plasmid was utilized to construct BRD7 knockin HSC lines. Transfection efficiency was verified by real-time PCR (n = 3 in every group, ***P < 0.001) (B–F) BRD7 knockout and knockin HSC lines were exposed to erastin (10 μM) or RSL3 (2.5 μM) for 24 h. Cell death was determined by CCK8 kit (n = 3 in every group, *P < 0.05). The levels of iron, MDA, lipid ROS, and GSH were determined by commercialized kits (n = 3 in every group, *P < 0.05). (G) BRD7 wild type (WT) and knockout (KO) HSC-LX2 cells were exposed to erastin (10 μM) for 24 h. The typical changes of ferroptotic cells were examined by transmission electron microscope. (H) BRD7 knockin HSC lines were exposed to erastin (10 μM) with or without the indicated inhibitors (Deferoxamine, 100 μM; eugenol, 2 μM; ZVAD-FMK, 10 μM; necrostatin-1, 10 μM; necrosulfonamide, 5 μM) for 24 h. Cell viability were determined by CCK8 kit (n = 3 in every group, ***P < 0.001).

Article Snippet: Necrostatin-1 (S8037), ferrostatin-1 (S7243), Z-VAD-FMK (S7023), RSL3 (S8155), erastin (S7242), deferoxamine (S5742), eugenol (S4706), necrostatin-1 (S8037), necrosulfonamide (S8251), and rotenone (S2348) were bought from Selleck Chemicals.

Techniques: Modification, CRISPR, Knock-Out, Plasmid Preparation, Construct, Knock-In, Transfection, Real-time Polymerase Chain Reaction, Transmission Assay, Microscopy

Journal: Molecules

Article Title: Effect of Fermented Matrix on the Color and Stability of Strawberry and Blueberry Anthocyanins during the Storage of Fruit Yogurts and Soy-Based and Bean-Based Fruit Yogurt Alternatives

doi: 10.3390/molecules28176222

Figure Lengend Snippet: Changes in anthocyanin content during the storage of strawberry yogurts and plant-based yogurt alternatives and the kinetic parameters for the degradation of anthocyanins.

Article Snippet: Delphinidin-3- O -glucoside, cyanidin-3- O -glucoside, pelargonidin-3- O -glucoside, pelargonidin-3- O -rutinoside were obtained from Carbosynth Ltd. (Compton, UK), cyanidin-3- O -galactoside was purchased from Extrasynthese (Lyon, France).

Techniques: