ethyl nitrosourea Search Results


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SERVA Electrophoresis n -ethyl- n -nitroso urea (enu)
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Gentronix n-ethyl-n-nitrosourea
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Ingenium Pharmaceuticals GmbH phospho1 -inactivating mutations of the n -ethyl- n -nitrosourea (enu) mutagenesis sperm bank collection
Effect of increasing concentrations of ATP ( A ), ADP ( B ), and PP i ( C ) on the P i -generating activity of the MVs: WT (•); TNAP-deficient (○); NPP1-deficient (□), and <t>PHOSPHO1-deficient</t> (Δ). Assays were done at 37°C and buffered with 50 mmol/L Tris HCl, pH 7.4, containing 2 mmol/L MgCl 2 and substrate and released P i measured as described in “Materials and Methods.”
Phospho1 Inactivating Mutations Of The N Ethyl N Nitrosourea (Enu) Mutagenesis Sperm Bank Collection, supplied by Ingenium Pharmaceuticals GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BEHRINGER International GmbH n-ethyl-n-nitrosourea-induced gonadotropin-releasing hormone receptor gene mutation
Effect of increasing concentrations of ATP ( A ), ADP ( B ), and PP i ( C ) on the P i -generating activity of the MVs: WT (•); TNAP-deficient (○); NPP1-deficient (□), and <t>PHOSPHO1-deficient</t> (Δ). Assays were done at 37°C and buffered with 50 mmol/L Tris HCl, pH 7.4, containing 2 mmol/L MgCl 2 and substrate and released P i measured as described in “Materials and Methods.”
N Ethyl N Nitrosourea Induced Gonadotropin Releasing Hormone Receptor Gene Mutation, supplied by BEHRINGER International GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Transposagen n-ethyl-n-nitrosourea (enu)-induced mutation to the ghrelin receptor gene (ghs-r ko)
Effect of increasing concentrations of ATP ( A ), ADP ( B ), and PP i ( C ) on the P i -generating activity of the MVs: WT (•); TNAP-deficient (○); NPP1-deficient (□), and <t>PHOSPHO1-deficient</t> (Δ). Assays were done at 37°C and buffered with 50 mmol/L Tris HCl, pH 7.4, containing 2 mmol/L MgCl 2 and substrate and released P i measured as described in “Materials and Methods.”
N Ethyl N Nitrosourea (Enu) Induced Mutation To The Ghrelin Receptor Gene (Ghs R Ko), supplied by Transposagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Phenomix Corporation n-ethyl-n-nitrosourea (enu) mutagenesis
Effect of increasing concentrations of ATP ( A ), ADP ( B ), and PP i ( C ) on the P i -generating activity of the MVs: WT (•); TNAP-deficient (○); NPP1-deficient (□), and <t>PHOSPHO1-deficient</t> (Δ). Assays were done at 37°C and buffered with 50 mmol/L Tris HCl, pH 7.4, containing 2 mmol/L MgCl 2 and substrate and released P i measured as described in “Materials and Methods.”
N Ethyl N Nitrosourea (Enu) Mutagenesis, supplied by Phenomix Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pfaltz & Bauer n-ethyl-n-nitrosourea
Effect of increasing concentrations of ATP ( A ), ADP ( B ), and PP i ( C ) on the P i -generating activity of the MVs: WT (•); TNAP-deficient (○); NPP1-deficient (□), and <t>PHOSPHO1-deficient</t> (Δ). Assays were done at 37°C and buffered with 50 mmol/L Tris HCl, pH 7.4, containing 2 mmol/L MgCl 2 and substrate and released P i measured as described in “Materials and Methods.”
N Ethyl N Nitrosourea, supplied by Pfaltz & Bauer, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nacalai n ethyl n nitrosourea
Effect of increasing concentrations of ATP ( A ), ADP ( B ), and PP i ( C ) on the P i -generating activity of the MVs: WT (•); TNAP-deficient (○); NPP1-deficient (□), and <t>PHOSPHO1-deficient</t> (Δ). Assays were done at 37°C and buffered with 50 mmol/L Tris HCl, pH 7.4, containing 2 mmol/L MgCl 2 and substrate and released P i measured as described in “Materials and Methods.”
N Ethyl N Nitrosourea, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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N/A
N-Nitroso-N-ethylurea is a precursor of Diazoethane with alkylating, carcinogenic, and mutagenic properties.A precursor of Diazoethane
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Effect of increasing concentrations of ATP ( A ), ADP ( B ), and PP i ( C ) on the P i -generating activity of the MVs: WT (•); TNAP-deficient (○); NPP1-deficient (□), and PHOSPHO1-deficient (Δ). Assays were done at 37°C and buffered with 50 mmol/L Tris HCl, pH 7.4, containing 2 mmol/L MgCl 2 and substrate and released P i measured as described in “Materials and Methods.”

Journal: Journal of Bone and Mineral Research

Article Title: Kinetic Analysis of Substrate Utilization by Native and TNAP-, NPP1-, or PHOSPHO1-Deficient Matrix Vesicles

doi: 10.1359/jbmr.091023

Figure Lengend Snippet: Effect of increasing concentrations of ATP ( A ), ADP ( B ), and PP i ( C ) on the P i -generating activity of the MVs: WT (•); TNAP-deficient (○); NPP1-deficient (□), and PHOSPHO1-deficient (Δ). Assays were done at 37°C and buffered with 50 mmol/L Tris HCl, pH 7.4, containing 2 mmol/L MgCl 2 and substrate and released P i measured as described in “Materials and Methods.”

Article Snippet: The Phospho1 −/− mice were identified and selected from a screen of Phospho1 -inactivating mutations of the N -ethyl- N -nitrosourea (ENU) mutagenesis sperm bank collection of Ingenium Pharmaceuticals AG (Martinsried, Germany).

Techniques: Activity Assay

Amounts of hydrolyzed ATP and produced ADP and AMP by MVs: WT (•), TNAP-deficient (○), NPP1-deficient (□), and PHOSPHO1-deficient (Δ), monitored by HPLC analysis as described in “Materials and Methods.”

Journal: Journal of Bone and Mineral Research

Article Title: Kinetic Analysis of Substrate Utilization by Native and TNAP-, NPP1-, or PHOSPHO1-Deficient Matrix Vesicles

doi: 10.1359/jbmr.091023

Figure Lengend Snippet: Amounts of hydrolyzed ATP and produced ADP and AMP by MVs: WT (•), TNAP-deficient (○), NPP1-deficient (□), and PHOSPHO1-deficient (Δ), monitored by HPLC analysis as described in “Materials and Methods.”

Article Snippet: The Phospho1 −/− mice were identified and selected from a screen of Phospho1 -inactivating mutations of the N -ethyl- N -nitrosourea (ENU) mutagenesis sperm bank collection of Ingenium Pharmaceuticals AG (Martinsried, Germany).

Techniques: Produced

Amounts of hydrolyzed ADP and produced AMP for MVs: WT (•), TNAP-deficient (○), NPP1-deficient (□), and PHOSPHO1-deficient (Δ), monitored by HPLC analysis as described in “Materials and Methods.”

Journal: Journal of Bone and Mineral Research

Article Title: Kinetic Analysis of Substrate Utilization by Native and TNAP-, NPP1-, or PHOSPHO1-Deficient Matrix Vesicles

doi: 10.1359/jbmr.091023

Figure Lengend Snippet: Amounts of hydrolyzed ADP and produced AMP for MVs: WT (•), TNAP-deficient (○), NPP1-deficient (□), and PHOSPHO1-deficient (Δ), monitored by HPLC analysis as described in “Materials and Methods.”

Article Snippet: The Phospho1 −/− mice were identified and selected from a screen of Phospho1 -inactivating mutations of the N -ethyl- N -nitrosourea (ENU) mutagenesis sperm bank collection of Ingenium Pharmaceuticals AG (Martinsried, Germany).

Techniques: Produced

Schematic representation of the most favored enzymatic reactions in the MV compartment at physiologic pH. We surmise that HA seed crystals are formed in the sheltered interior of MVs favored by the P i -generating ability of PHOSPHO1, as well as by the function of Pit1/2 phosphate transporters. While NPP1 clearly has a role in PP i -generation at the level of the chondrocyte and osteoblast membranes, at the level of MVs NPP1 does not use ATP efficiently. Instead, it is TNAP that has a major role both as a PP i ase and an ATPase/ADPase and thus participates in the calcification process both by restricting the concentration of extracellular PP i and by also contributing to the P i pool available for calcification. Thick lines indicate the most favored reactions, whereas dotted lines indicate alternative enzymatic pathways, such as the ability of NPP1 to act as a phosphatase.

Journal: Journal of Bone and Mineral Research

Article Title: Kinetic Analysis of Substrate Utilization by Native and TNAP-, NPP1-, or PHOSPHO1-Deficient Matrix Vesicles

doi: 10.1359/jbmr.091023

Figure Lengend Snippet: Schematic representation of the most favored enzymatic reactions in the MV compartment at physiologic pH. We surmise that HA seed crystals are formed in the sheltered interior of MVs favored by the P i -generating ability of PHOSPHO1, as well as by the function of Pit1/2 phosphate transporters. While NPP1 clearly has a role in PP i -generation at the level of the chondrocyte and osteoblast membranes, at the level of MVs NPP1 does not use ATP efficiently. Instead, it is TNAP that has a major role both as a PP i ase and an ATPase/ADPase and thus participates in the calcification process both by restricting the concentration of extracellular PP i and by also contributing to the P i pool available for calcification. Thick lines indicate the most favored reactions, whereas dotted lines indicate alternative enzymatic pathways, such as the ability of NPP1 to act as a phosphatase.

Article Snippet: The Phospho1 −/− mice were identified and selected from a screen of Phospho1 -inactivating mutations of the N -ethyl- N -nitrosourea (ENU) mutagenesis sperm bank collection of Ingenium Pharmaceuticals AG (Martinsried, Germany).

Techniques: Concentration Assay