erk2 Search Results


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R&D Systems anti tnf α mab 1825 12
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Proteintech erk
Fig. 4. Effects of AM1241 on the NF-κB and MAPK signaling pathways. (A): EMSA technique to examine NF-κB transcriptional activity. (B): Western blot analysis to detect the phosphorylation of MAPKs <t>(ERK,</t> <t>JNK,</t> <t>P38).</t> n=3, *P<0.05, **P<0.01, ***P<0.001. n=3.
Erk, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. Effects of AM1241 on the NF-κB and MAPK signaling pathways. (A): EMSA technique to examine NF-κB transcriptional activity. (B): Western blot analysis to detect the phosphorylation of MAPKs <t>(ERK,</t> <t>JNK,</t> <t>P38).</t> n=3, *P<0.05, **P<0.01, ***P<0.001. n=3.
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Fig. 4. Effects of AM1241 on the NF-κB and MAPK signaling pathways. (A): EMSA technique to examine NF-κB transcriptional activity. (B): Western blot analysis to detect the phosphorylation of MAPKs <t>(ERK,</t> <t>JNK,</t> <t>P38).</t> n=3, *P<0.05, **P<0.01, ***P<0.001. n=3.
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Fig. 4. Effects of AM1241 on the NF-κB and MAPK signaling pathways. (A): EMSA technique to examine NF-κB transcriptional activity. (B): Western blot analysis to detect the phosphorylation of MAPKs <t>(ERK,</t> <t>JNK,</t> <t>P38).</t> n=3, *P<0.05, **P<0.01, ***P<0.001. n=3.
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Cell Signaling Technology Inc anti phospho p44 42 mapk
Fig. 4. Effects of AM1241 on the NF-κB and MAPK signaling pathways. (A): EMSA technique to examine NF-κB transcriptional activity. (B): Western blot analysis to detect the phosphorylation of MAPKs <t>(ERK,</t> <t>JNK,</t> <t>P38).</t> n=3, *P<0.05, **P<0.01, ***P<0.001. n=3.
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Fig. 4. Effects of AM1241 on the NF-κB and MAPK signaling pathways. (A): EMSA technique to examine NF-κB transcriptional activity. (B): Western blot analysis to detect the phosphorylation of MAPKs <t>(ERK,</t> <t>JNK,</t> <t>P38).</t> n=3, *P<0.05, **P<0.01, ***P<0.001. n=3.
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R&D Systems anti erk 1 2
Fig. 4. Effects of AM1241 on the NF-κB and MAPK signaling pathways. (A): EMSA technique to examine NF-κB transcriptional activity. (B): Western blot analysis to detect the phosphorylation of MAPKs <t>(ERK,</t> <t>JNK,</t> <t>P38).</t> n=3, *P<0.05, **P<0.01, ***P<0.001. n=3.
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Addgene inc pcmv5 myc erk2
Fig. 4. Effects of AM1241 on the NF-κB and MAPK signaling pathways. (A): EMSA technique to examine NF-κB transcriptional activity. (B): Western blot analysis to detect the phosphorylation of MAPKs <t>(ERK,</t> <t>JNK,</t> <t>P38).</t> n=3, *P<0.05, **P<0.01, ***P<0.001. n=3.
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Cell Signaling Technology Inc signalsilencer p42 mapk erk2 sirna ii
Monocytes were nucleofected with ERK1 and <t>ERK2</t> specific <t>siRNA</t> or scrambled control and recovered overnight. Cells were then primed with LPS (1μg/ml) for 30 min and activated with ATP (5mM) for another 30 min. Supernatants were analyzed for IL-18 release and cell lysates were immunoblotted to confirm ERK protein down regulation using total ERK antibody. IL-18 ELISA represents the mean± SEM for three separate monocyte donors and the blots are representative of repeated blots. **p < 0.01 as compared to scrambled siRNA.
Signalsilencer P42 Mapk Erk2 Sirna Ii, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 4. Effects of AM1241 on the NF-κB and MAPK signaling pathways. (A): EMSA technique to examine NF-κB transcriptional activity. (B): Western blot analysis to detect the phosphorylation of MAPKs (ERK, JNK, P38). n=3, *P<0.05, **P<0.01, ***P<0.001. n=3.

Journal: Brain research bulletin

Article Title: The effects and mechanisms of AM1241 in alleviating cerebral ischemia-reperfusion injury.

doi: 10.1016/j.brainresbull.2024.111025

Figure Lengend Snippet: Fig. 4. Effects of AM1241 on the NF-κB and MAPK signaling pathways. (A): EMSA technique to examine NF-κB transcriptional activity. (B): Western blot analysis to detect the phosphorylation of MAPKs (ERK, JNK, P38). n=3, *P<0.05, **P<0.01, ***P<0.001. n=3.

Article Snippet: Primary antibodies against p-p38 (28796–1-AP, Proteintech), p38 (14064–1-AP, Proteintech), p-ERK (28733–1-AP, Proteintech), ERK (11257–1-AP, Proteintech), p-JNK (80024–1-RR, Proteintech), JNK (66210–1-Ig, Proteintech), IL-6 (ab233706, Abcam), TNF-α (ab183218, Abcam), NOX2 (ab310337, Abcam), BAX (50599–2- Ig, Proteintech), Cleaved-Caspase3 (C-Caspase3) (25128–1-AP, Proteintech) were added and incubated at 4◦C overnight.

Techniques: Protein-Protein interactions, Activity Assay, Western Blot, Phospho-proteomics

Monocytes were nucleofected with ERK1 and ERK2 specific siRNA or scrambled control and recovered overnight. Cells were then primed with LPS (1μg/ml) for 30 min and activated with ATP (5mM) for another 30 min. Supernatants were analyzed for IL-18 release and cell lysates were immunoblotted to confirm ERK protein down regulation using total ERK antibody. IL-18 ELISA represents the mean± SEM for three separate monocyte donors and the blots are representative of repeated blots. **p < 0.01 as compared to scrambled siRNA.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Inflammasome priming by LPS is dependent upon ERK signaling and proteasome function *

doi: 10.4049/jimmunol.1301974

Figure Lengend Snippet: Monocytes were nucleofected with ERK1 and ERK2 specific siRNA or scrambled control and recovered overnight. Cells were then primed with LPS (1μg/ml) for 30 min and activated with ATP (5mM) for another 30 min. Supernatants were analyzed for IL-18 release and cell lysates were immunoblotted to confirm ERK protein down regulation using total ERK antibody. IL-18 ELISA represents the mean± SEM for three separate monocyte donors and the blots are representative of repeated blots. **p < 0.01 as compared to scrambled siRNA.

Article Snippet: In knockdown experiments, small interfering RNA (siRNA) against ERK1 and scrambled siRNA were purchased from Sigma-Aldrich while Signalsilencer ® p42 MAPK (ERK2) siRNA II was purchased from Cell Signaling Company. siRNAs were delivered in monocytes by Amaxa nucleofector I (Lonza).

Techniques: Enzyme-linked Immunosorbent Assay