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Image Search Results
Journal: Brain research bulletin
Article Title: The effects and mechanisms of AM1241 in alleviating cerebral ischemia-reperfusion injury.
doi: 10.1016/j.brainresbull.2024.111025
Figure Lengend Snippet: Fig. 4. Effects of AM1241 on the NF-κB and MAPK signaling pathways. (A): EMSA technique to examine NF-κB transcriptional activity. (B): Western blot analysis to detect the phosphorylation of MAPKs (ERK, JNK, P38). n=3, *P<0.05, **P<0.01, ***P<0.001. n=3.
Article Snippet: Primary antibodies against p-p38 (28796–1-AP, Proteintech), p38 (14064–1-AP, Proteintech), p-ERK (28733–1-AP, Proteintech),
Techniques: Protein-Protein interactions, Activity Assay, Western Blot, Phospho-proteomics
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Inflammasome priming by LPS is dependent upon ERK signaling and proteasome function
doi: 10.4049/jimmunol.1301974
Figure Lengend Snippet: Monocytes were nucleofected with ERK1 and ERK2 specific siRNA or scrambled control and recovered overnight. Cells were then primed with LPS (1μg/ml) for 30 min and activated with ATP (5mM) for another 30 min. Supernatants were analyzed for IL-18 release and cell lysates were immunoblotted to confirm ERK protein down regulation using total ERK antibody. IL-18 ELISA represents the mean± SEM for three separate monocyte donors and the blots are representative of repeated blots. **p < 0.01 as compared to scrambled siRNA.
Article Snippet: In knockdown experiments, small interfering RNA (siRNA) against ERK1 and scrambled siRNA were purchased from Sigma-Aldrich while
Techniques: Enzyme-linked Immunosorbent Assay