Journal: PLoS ONE

Article Title: Up-Regulation of hERG K + Channels by B-RAF

doi: 10.1371/journal.pone.0087457

Figure Lengend Snippet: A. Representative original western blot showing hERG membrane protein abundance (anti-K v 11.1 antibody, Alamone Labs) analyzed by cell surface biotinylation in rhabdomyosarcoma RD cells after 24 hours treatment with vehicle alone (Control) or with 10 µM B-RAF inhibitor PLX-4720 (PLX-4720). B. Arithmetic means ± SEM (n = 7, arbitrary units) of normalized hERG membrane protein abundance analyzed by cell surface biotinylation in rhabdomyosarcoma RD cells after 24 hours treatment with vehicle alone (white bar) or with 10 µM B-RAF inhibitor PLX-4720 (black bar). *(p<0.05) indicates statistically significant difference from rhabdomyosarcoma RD cells treated with vehicle alone. C. Representative original dot plots of hERG-FITC positive cells at the cell surface analysed by flow cytometry in rhabdomyosarcoma RD cells after 24 hours treatment with vehicle alone (Control) or with 10 µM B-RAF inhibitor PLX-4720 (PLX-4720); FL-1 Height: hERG-FITC fluorescence intensity. D. Arithmetic means ± SEM (n = 5, %) of normalized percentage of positive cells showing hERG expression at the cell surface analyzed by flow cytometry in rhabdomyosarcoma RD cells after 24 hours treatment with vehicle alone (white bar) or with 10 µM B-RAF inhibitor PLX-4720 (black bar). *(p<0.05) indicates statistically significant difference from rhabdomyosarcoma RD cells treated with vehicle alone.

Article Snippet: After blocking with 5% non-fat dry milk in TBS 0.1% Tween20 for 1 hour at RT, the blots were incubated overnight at 4°C with rabbit anti-K v 11.1 (hERG, extracellular) antibody (diluted 1:200, Alamone Labs, Jerusalem, Israel).

Techniques: Western Blot, Flow Cytometry, Fluorescence, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Transcriptome Profiling of Prostate Cancer, Considering Risk Groups and the TMPRSS2-ERG Molecular Subtype

doi: 10.3390/ijms24119282

Figure Lengend Snippet: Heatmap of top 50 differentially expressed genes between LAPCa and LPCa for the TMPRSS2-ERG subtype. LAPCa—locally advanced PCa; LPCa—localized PCa. Red color indicates upregulated genes, blue—downregulated.

Article Snippet: The TaqMan Gene Expression Assay Hs03063375_ft (Thermo Fisher Scientific) was used to determine the presence of the TMPRSS2-ERG fusion transcript.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Transcriptome Profiling of Prostate Cancer, Considering Risk Groups and the TMPRSS2-ERG Molecular Subtype

doi: 10.3390/ijms24119282

Figure Lengend Snippet: Dotplot of GSEA results illustrating KEGG-based biological processes associated with the LAPCa category versus LPCa for the high-risk group within the TMPRSS2-ERG molecular subtype. The figure shows statistically significantly enriched biological pathways based on the coexpression of genes. NES—normalized enrichment score.

Article Snippet: The TaqMan Gene Expression Assay Hs03063375_ft (Thermo Fisher Scientific) was used to determine the presence of the TMPRSS2-ERG fusion transcript.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Transcriptome Profiling of Prostate Cancer, Considering Risk Groups and the TMPRSS2-ERG Molecular Subtype

doi: 10.3390/ijms24119282

Figure Lengend Snippet: Differential expression of genes statistically significantly associated with the LAPCa group, including within the TMPRSS2-ERG subtype. LAPCa—locally advanced prostate cancer, LPCa—localized prostate cancer, Log2FC—log2 transformed fold change, r s —Spearman correlation coefficient, grey background—downregulated genes.

Article Snippet: The TaqMan Gene Expression Assay Hs03063375_ft (Thermo Fisher Scientific) was used to determine the presence of the TMPRSS2-ERG fusion transcript.

Techniques: Quantitative Proteomics, Transformation Assay, Ubiquitin Proteomics, Binding Assay

Journal: International Journal of Molecular Sciences

Article Title: Transcriptome Profiling of Prostate Cancer, Considering Risk Groups and the TMPRSS2-ERG Molecular Subtype

doi: 10.3390/ijms24119282

Figure Lengend Snippet: Results of ROC analysis based on the logistic regression algorithm for test data based on a cohort of Russian patients with high-risk LPCa and LAPCa, considering the TMPRSS2-ERG molecular subtype. LAPCa—locally advanced prostate cancer, LPCa—localized prostate cancer, AUC—Area Under Curve, CI—confidence interval, grey background—downregulated genes.

Article Snippet: The TaqMan Gene Expression Assay Hs03063375_ft (Thermo Fisher Scientific) was used to determine the presence of the TMPRSS2-ERG fusion transcript.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Transcriptome Profiling of Prostate Cancer, Considering Risk Groups and the TMPRSS2-ERG Molecular Subtype

doi: 10.3390/ijms24119282

Figure Lengend Snippet: Clinical and pathological characteristics of the studied cohort. pT—primary tumor estimation; N—regional lymph nodes; M—distant metastases; ISUP—The International Society of Urological Pathology.

Article Snippet: The TaqMan Gene Expression Assay Hs03063375_ft (Thermo Fisher Scientific) was used to determine the presence of the TMPRSS2-ERG fusion transcript.

Techniques: