Journal: bioRxiv

Article Title: Rapid differentiation of estrogen receptor status in patient biopsy breast cancer aspirates with an optical nanosensor

doi: 10.1101/2024.03.29.587397

Figure Lengend Snippet: (A) Schematic of the ERα antibody-based NIR fluorescent nanosensor detection concept. (B) NIR fluorescent spectra of the construct nanosensor in PBS. Success of the antibody conjugation to base construct was assessed by (C) comparison of decay in correlation coefficient as a function of time for SWCNT-(TAT) 6 -NH 2 and ERα antibody (Ab) conjugated to SWCNT- (TAT) 6 , (D) Change in zeta potential for ssDNA-SWCNT compared to the ERα nanosensor. Difference in means = 9.1 mV, p = 0.006.

Article Snippet: To initially evaluate the response of the ERα nanosensor complex in buffer, we first measured the fluorescence response of 0.5 mg/L nanosensor to 250 nM human ERα (Catalog number NBP2-34478PEP, Novus Biologicals; Colorado, USA) in 1x PBS using a near-infrared (NIR) fluorescent cuvette-based spectrometer (NS MiniTracer; Applied NanoFluorescence, Texas, USA).

Techniques: Construct, Conjugation Assay, Comparison, Zeta Potential Analyzer

Journal: bioRxiv

Article Title: Rapid differentiation of estrogen receptor status in patient biopsy breast cancer aspirates with an optical nanosensor

doi: 10.1101/2024.03.29.587397

Figure Lengend Snippet: (A) Response of the nanosensor (7,6) chirality to 250 nM recombinant ERα in PBS. Difference in means = 3.5 nm, p = 0.004. (B) Response of the nanosensor (7,6) chirality to 250 nM recombinant ERα in 10% FBS. Difference in means = 1 nm, p = 0.002. (C) Change in center wavelength of the (7,5) chirality for the nanosensor incubated with ER- or ER+ cells. Difference in means = 5.5 nm, p = 0.04).

Article Snippet: To initially evaluate the response of the ERα nanosensor complex in buffer, we first measured the fluorescence response of 0.5 mg/L nanosensor to 250 nM human ERα (Catalog number NBP2-34478PEP, Novus Biologicals; Colorado, USA) in 1x PBS using a near-infrared (NIR) fluorescent cuvette-based spectrometer (NS MiniTracer; Applied NanoFluorescence, Texas, USA).

Techniques: Recombinant, Incubation

Journal: bioRxiv

Article Title: Rapid differentiation of estrogen receptor status in patient biopsy breast cancer aspirates with an optical nanosensor

doi: 10.1101/2024.03.29.587397

Figure Lengend Snippet: (A) Schematic of direct nanosensor response measurement to ER+ breast cancer patient cells. (B) Change in center wavelength of the nanosensor (7,5) chirality after incubation with ER- or ER+ breast cancer biopsy aspirates. Difference in means = 2.3 nm, p = 0.0011. (C) Change in center wavelength of the nanosensor (7,6) chirality after incubation with ER- or ER+ breast cancer biopsy aspirates. Difference in means = 1.6 nm, p = 0.0002. (D) Change in center wavelength of the nanosensor (9,4) chirality after incubation with ER- or ER+ breast cancer biopsy aspirates. Difference in means = 1.2 nm, p = 0.0001. (E) Receiver operating characteristic evaluation of the ability of each chirality to differentiate ER-from ER+ biopsy samples. AUC is area under the curve. p(7,5) = 0.0020, (7,6) = 0.017, (9,4) = 0.0029.

Article Snippet: To initially evaluate the response of the ERα nanosensor complex in buffer, we first measured the fluorescence response of 0.5 mg/L nanosensor to 250 nM human ERα (Catalog number NBP2-34478PEP, Novus Biologicals; Colorado, USA) in 1x PBS using a near-infrared (NIR) fluorescent cuvette-based spectrometer (NS MiniTracer; Applied NanoFluorescence, Texas, USA).

Techniques: Incubation

Journal: bioRxiv

Article Title: Rapid differentiation of estrogen receptor status in patient biopsy breast cancer aspirates with an optical nanosensor

doi: 10.1101/2024.03.29.587397

Figure Lengend Snippet: (A) Schematic representing the fractionation method to measure the change in center wavelength for bound vs. unbound nanosensor. (B) Change in center wavelength of the nanosensor each chirality of the “Washed Bound” fraction. Only the (7,6) chirality (center) exhibited a significant change. Difference in the means for this sample = 3.1 nm, p = 0.025. (C) Change in center wavelength of the nanosensor each chirality of the “Washed Unbound” fraction. No differences were statistically significant. (D) ROC comparison of the three measurements (from & ) and the ability of each chirality to differentiate ER-from ER+ cells. Only the Unwashed method was statistically significant (please refer to for AUC and p values for each).

Article Snippet: To initially evaluate the response of the ERα nanosensor complex in buffer, we first measured the fluorescence response of 0.5 mg/L nanosensor to 250 nM human ERα (Catalog number NBP2-34478PEP, Novus Biologicals; Colorado, USA) in 1x PBS using a near-infrared (NIR) fluorescent cuvette-based spectrometer (NS MiniTracer; Applied NanoFluorescence, Texas, USA).

Techniques: Fractionation, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Glyphosate Exposure Induces Cytotoxicity, Mitochondrial Dysfunction and Activation of ERα and ERβ Estrogen Receptors in Human Prostate PNT1A Cells

doi: 10.3390/ijms25137039

Figure Lengend Snippet: Graphs showing the results of immunofluorescence intensity analysis of ERs in the untreated PNT1A (CTRL) and in cells exposed to Gly (3.5 × 10 −4 M) alone and in the presence of the inhibitor tamoxifen for different times. ( A ) ERα levels. ( B ) ERβ levels. Legend: C—cytoplasm; N—nucleus. Asterisks indicate statistically significant differences between receptor levels in the cytoplasm and nucleus for a specific exposure time: ** p < 0.01; *** p < 0.001, **** p < 0.0001.

Article Snippet: Afterwards, the chambers were incubated for 1 h at room temperature with the primary anti-ERα antibody (E-AB-66893 Elabscience rabbit polyclonal) and the anti-ERβ (SC-8974 Santacruz rabbit polyclonal), both diluted 1:100 in 1% BSA.

Techniques: Immunofluorescence

Journal: International Journal of Molecular Sciences

Article Title: Glyphosate Exposure Induces Cytotoxicity, Mitochondrial Dysfunction and Activation of ERα and ERβ Estrogen Receptors in Human Prostate PNT1A Cells

doi: 10.3390/ijms25137039

Figure Lengend Snippet: Western blot analysis of ERs levels in cytosolic and nuclear protein extracts of PNT1A cells. Representative Western blots of nuclear (N) and cytosolic (C) homogenates from control cells (CTRL) and cells treated for 30′, 2 h, and 4 h with 3.5 × 10 −4 M (LD) Gly, stained with anti-ERα, anti-ERβ, anti-GAPDH, and anti-PARP. ( A , B ) Graphs showing the quantitative results for ERα ( A ) and ERβ ( B ). Nuclear protein level was normalized to PARP levels; cytosolic protein level was normalized to GAPDH levels. Values are means ± SEM of three independent experiments. Asterisks indicate statistically significant differences compared to control cells: ** p < 0.01; *** p < 0.001, **** p < 0.0001.

Article Snippet: Afterwards, the chambers were incubated for 1 h at room temperature with the primary anti-ERα antibody (E-AB-66893 Elabscience rabbit polyclonal) and the anti-ERβ (SC-8974 Santacruz rabbit polyclonal), both diluted 1:100 in 1% BSA.

Techniques: Western Blot, Control, Staining