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Image Search Results
Journal: Cell Proliferation
Article Title: Expansion of mouse sertoli cells on microcarriers
doi: 10.1111/j.1365-2184.2010.00677.x
Figure Lengend Snippet: Effect of inoculum on viable cell density of Sertoli cells (SCs). Effect of inoculum on viable cell density for SCs grown in: (a) stirred microcarrier cultures and (b) control plates. Cells were plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in 80 or 2.5 ml of DMEM, for the stirred and control cultures, respectively. For stirred cultures, 3 mg of Cytodex‐1 per mL was used. □, ○, △ stand for viabilities (%) of SCs, plated at () 0.5 × 105, (•) 1 × 105 and () 2 × 105 cells/ml in the figure. Media (50%) was changed every day from day 2.
Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (
Techniques: Control
Journal: Cell Proliferation
Article Title: Expansion of mouse sertoli cells on microcarriers
doi: 10.1111/j.1365-2184.2010.00677.x
Figure Lengend Snippet: Initial and final cell densities, and fold increase of Sertoli cells grown in stirred microcarrier cultures and six‐well control plates
Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (
Techniques: Control
Journal: Cell Proliferation
Article Title: Expansion of mouse sertoli cells on microcarriers
doi: 10.1111/j.1365-2184.2010.00677.x
Figure Lengend Snippet: Available surface areas estimated in for stirred microcarrier bead cultures and control plates
Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (
Techniques: Control, Concentration Assay
Journal: Cell Proliferation
Article Title: Expansion of mouse sertoli cells on microcarriers
doi: 10.1111/j.1365-2184.2010.00677.x
Figure Lengend Snippet: Apparent ( μ app ) and maximum ( μ max ) specific growth rates calculated using first order kinetic model for Sertoli cells (SCs) grown in stirred microcarrier cultures and control plates.
Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (
Techniques: Control
Journal: Cell Proliferation
Article Title: Expansion of mouse sertoli cells on microcarriers
doi: 10.1111/j.1365-2184.2010.00677.x
Figure Lengend Snippet: Microscopic observation of Sertoli cells grown at different microcarrier concentrations. Sertoli cells (SCs) plated at 1 × 105 cells/ml in 80 ml of DMEM with different microcarrier bead concentrations, observed on the 2nd day after inoculation.
Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (
Techniques:
Journal: Cell Proliferation
Article Title: Expansion of mouse sertoli cells on microcarriers
doi: 10.1111/j.1365-2184.2010.00677.x
Figure Lengend Snippet: Substrate concentration and metabolite profiles during culture of Sertoli cells (SCs) on Cytodex‐1 microcarrier beads. Concentration (, •, , ) and specific production/consumption (□, ○, △, ▽) rates of glucose (a), lactate (b), glutamine (c) and ammonia (d) in culture represented. Values displayed represent average of three independent experiments. Error bars indicate the standard deviation of duplicate cultures. SCs (1 × 105 cells/ml) cultured with microcarrier bead concentration of 3 mg/ml. Media (50%) was changed every day, starting on day 2.
Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (
Techniques: Concentration Assay, Standard Deviation, Cell Culture
Journal: Cell Proliferation
Article Title: Expansion of mouse sertoli cells on microcarriers
doi: 10.1111/j.1365-2184.2010.00677.x
Figure Lengend Snippet: Optical and scanning electron micrographs of Sertoli cells (SCs) cultured on microcarrier beads under stirred culture conditions. SCs visualized using an optical microscope, day 2 (a), day 4 (b), day 6 (c) and day 8 (d) after MTT staining (200× amplification) for Cytodex‐1, respectively. SCs visualized using a scanning electron microscope, day 2 (e), day 4 (f), day 6 (g) and day 8 (h).
Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (
Techniques: Cell Culture, Microscopy, Staining, Amplification
Journal: Cell Proliferation
Article Title: Expansion of mouse sertoli cells on microcarriers
doi: 10.1111/j.1365-2184.2010.00677.x
Figure Lengend Snippet: Photomicrograph of neuronal stem cells (NSCs) cultured alone and NSCs co‐cultured with Sertoli cells (SCs). (a) NSCs cultured in six‐well chamber slides. (b) NSCs co‐cultured with SCs obtained from two‐dimensional culture. (c) NSCs co‐cultured with SCs obtained from microcarrier bead suspension culture. All photographed on day 3 after inoculation (the scale bar is 5 μm). Neurite outgrowth was marked with.
Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (
Techniques: Cell Culture, Suspension
Journal: Cell Proliferation
Article Title: Expansion of mouse sertoli cells on microcarriers
doi: 10.1111/j.1365-2184.2010.00677.x
Figure Lengend Snippet: Nerve stem cells (NSCs) immunocytofluorescently labelled with anti‐nestin antibody to identify neurospheres (day 6). (a) NSCs cultured on six‐well chamber slides. (b) NSCs cultured with Sertoli cells (SCs) obtained from two‐dimensional culture. (c) NSCs cultured with SCs obtained from microcarrier bead culture.
Article Snippet: One gram dry weight of this support has an approximate surface area of 4400 cm 2 (
Techniques: Cell Culture