Journal: Journal of Gastroenterology

Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

doi: 10.1007/s00535-025-02323-y

Figure Lengend Snippet: Predicted structures of the mutated TRPV6 protein based on the minigene assays. Normal splicing of the wild-type sequence and aberrant splicing of the mutant sequence are illustrated for each variant

Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

Techniques: Sequencing, Mutagenesis, Variant Assay

Journal: Journal of Gastroenterology

Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

doi: 10.1007/s00535-025-02323-y

Figure Lengend Snippet: Comparison of different pathogenic genotypes on clinical outcomes. Kaplan–Meier curves showing the cumulative rates of A symptom onset, B pancreatic calcification, C pancreatic exocrine insufficiency, and D diabetes mellitus, according to genotype ( TRPV6 -related, PRSS1 -related, SPINK1 -related, or PV-negative pancreatitis). Censored subjects are indicated on the Kaplan–Meier curves by tick marks

Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

Techniques: Comparison

Journal: Journal of Gastroenterology

Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

doi: 10.1007/s00535-025-02323-y

Figure Lengend Snippet: Timing of the first interventions for pancreatitis and the diagnosis of pancreatic cancer according to pathogenic genotypes. Kaplan–Meier curves showing the cumulative rates of A endoscopic treatment, B surgery, C all interventions, and D pancreatic cancer diagnosis according to genotype ( TRPV6 -related, PRSS1 -related, SPINK1 -related, or PV-negative pancreatitis). Censored subjects are indicated on the Kaplan–Meier curves by tick marks

Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

Techniques: Biomarker Discovery

Journal: Journal of Gastroenterology

Article Title: TRPV6 -related pancreatitis: natural history and the impact of the pancreas-specific deletion on pancreatitis in mice

doi: 10.1007/s00535-025-02323-y

Figure Lengend Snippet: Caerulein-induced pancreatitis was exacerbated in pancreas-specific Trpv6 conditional knockout mice. A – D Trpv6 floxed mice and pancreas-specific Trpv6 CKO mice received eight hourly intraperitoneal injections of caerulein (100 μg/kg body weight) or saline for two consecutive days. Blood samples were collected at 0 h (just before the first caerulein injection), 8 h, 24 h, and 32 h, and mice were euthanized at either 32 h or 96 h. A Scheme of the experiments. B Blood samples were obtained at the indicated time points, and serum amylase levels were measured. C Representative H&E staining of the pancreas at 32 h. Scale bar = 100 μm. D Representative H&E staining and Sirius Red staining of the pancreas at 96 h after the first caerulein injection. E , F CP was induced by 6-hourly intraperitoneal injections of caerulein (100 μg/kg body weight), 3 days/week for four consecutive weeks. E Scheme of the experiments. F Representative H&E staining and Sirius Red staining of the pancreas after 4 weeks

Article Snippet: Slides were boiled in target retrieval solution (Dako, Glostrup, Denmark) and incubated with rabbit anti-TRPV6 antibody (ACC-036; Alomone Labs, Jerusalem, Israel) overnight at 4 °C.

Techniques: Knock-Out, Saline, Injection, Staining

Journal: Frontiers in oncology

Article Title: The Angiotensin-Converting Enzyme Inhibitory State Promotes the Transformation of Non-Small Cell Lung Cancer Blood Supply Pattern Toward Vasculogenic Mimicry Formation.

doi: 10.3389/fonc.2021.663671

Figure Lengend Snippet: FIGURE 1 | High ACE2 expression was linked to increased VM and better prognosis in NSCLC. (A) Typical image of ACE2, VE-cadherin, EphA2 protein expression and CD34/PAS double staining in TMA tissues. Case B1 had massive CD34−/PAS+ VM (yellow arrows) lined by ACE2, VE-cadherin and EphA2 high expressing tumor cells. Case G1 had abundant CD34+/PAS−MVs (black arrows) with ACE2, VE-cadherin and EphA2 low expressing tumor cells. (B) Kaplan-Meier analysis of OS in NSCLC patients with ACE2 low or high expression. P = 0.044.

Article Snippet: The sections were dewaxed, rehydrated, treated with antigen retrieval, hatched with primary antibodies of ACE2 (1:200), VE-cadherin (1:200), EphA2 (1:100), and CD34 (1:200, ab81289, Abcam) overnight at 4°C, individually exposed to secondary antibody (SA1020, BOSTER, USA) 1 h at RT, and colored with DAB peroxidase substrate.

Techniques: Expressing, Double Staining

Journal: Frontiers in oncology

Article Title: The Angiotensin-Converting Enzyme Inhibitory State Promotes the Transformation of Non-Small Cell Lung Cancer Blood Supply Pattern Toward Vasculogenic Mimicry Formation.

doi: 10.3389/fonc.2021.663671

Figure Lengend Snippet: FIGURE 5 | VM formation was increased, and vasculature was lessened due to inhibition of RAS in vivo. (A) Growth curve of allograft tumors of A549-ACE2-OE cells, A549-NC cells with or without ACEI treatment, Mean ± SD, n = 3, *p < 0.05. ns, no significance. (B) Weight of resected tumors, Mean ± SD, n = 3, **p < 0.01, *p < 0.05. (C) Continuous sections of allograft tumor tissues stained with PAS, CD34, VE-cadherin, or EphA2 immunohistochemical stain. Black arrow points out a typical MV (CD34+/PAS−); yellow arrows point out typical VM (CD34−/PAS+). (D) Quantification of MV and VM in different groups, Mean ± SD, n = 3, per field, ***p < 0.001, **p < 0.01. (E) Quantification of VE-cadherin and EphA2 mean optical density in three groups, Mean ± SD, n = 3, ***p < 0.001, **p < 0.01.

Article Snippet: The sections were dewaxed, rehydrated, treated with antigen retrieval, hatched with primary antibodies of ACE2 (1:200), VE-cadherin (1:200), EphA2 (1:100), and CD34 (1:200, ab81289, Abcam) overnight at 4°C, individually exposed to secondary antibody (SA1020, BOSTER, USA) 1 h at RT, and colored with DAB peroxidase substrate.

Techniques: Inhibition, In Vivo, Staining, Immunohistochemical staining

Journal: Frontiers in oncology

Article Title: The Angiotensin-Converting Enzyme Inhibitory State Promotes the Transformation of Non-Small Cell Lung Cancer Blood Supply Pattern Toward Vasculogenic Mimicry Formation.

doi: 10.3389/fonc.2021.663671

Figure Lengend Snippet: FIGURE 6 | VE-cadherin and EphA2 expression was upregulated in A549 cells and NSCLC tissues with impaired local RAS status. (A, B) RT-PCR experiment of VE-cadherin and EphA2 mRNA level in A549-ACE2-OE cells and control cells, Mean ± SD, n = 3, ***p < 0.001. (C–E) Western blot analysis and quantification of VE- cadherin and EphA2 expression level in A549-ACE2-OE cells and control cells, Mean ± SD, n = 3, ***p < 0.001. (F, G) Linear regressions of VM number and VE- cadherin (P < 0.0001) or EphA2 (P = 0.0108) score in TMA. (H) Typical tissue images of both groups stained with VE-cadherin, ACE2 or CD34/PAS. Case F13 with ACE2 low status was provided with rambling VM covered by tumor cells which only expressed VE-cadherin in nuclei; case D6 with ACE2 high status had ordered VM lined by tumor cells expressing VE-cadherin in both nuclei and cytomembranes. Red arrow: VE-cadherin membrane expression.

Article Snippet: The sections were dewaxed, rehydrated, treated with antigen retrieval, hatched with primary antibodies of ACE2 (1:200), VE-cadherin (1:200), EphA2 (1:100), and CD34 (1:200, ab81289, Abcam) overnight at 4°C, individually exposed to secondary antibody (SA1020, BOSTER, USA) 1 h at RT, and colored with DAB peroxidase substrate.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Western Blot, Staining, Membrane