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Fig. 1. Increased ROS in <t>RVFV</t> infected HepG2 cells. (A) HepG2 cells were infected with the MP-12 strain of RVFV (MOI: 3) and stained after 3 h using MitoSox stain and DAPI. The stained cells were visualized by confocal microscopy. (B) HepG2 cells infected with MP-12 (MOI: 3) were lysed at 24 h post infection and total protein lysate obtained. Lysates from uninfected cells were obtained as controls and both lysates were resolved by SDS-PAGE and western blots, carried out using anti-RVFV and <t>anti-β-actin</t> <t>antibodies.</t>
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Fig. 1. Increased ROS in <t>RVFV</t> infected HepG2 cells. (A) HepG2 cells were infected with the MP-12 strain of RVFV (MOI: 3) and stained after 3 h using MitoSox stain and DAPI. The stained cells were visualized by confocal microscopy. (B) HepG2 cells infected with MP-12 (MOI: 3) were lysed at 24 h post infection and total protein lysate obtained. Lysates from uninfected cells were obtained as controls and both lysates were resolved by SDS-PAGE and western blots, carried out using anti-RVFV and <t>anti-β-actin</t> <t>antibodies.</t>
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Fig. 1. Increased ROS in <t>RVFV</t> infected HepG2 cells. (A) HepG2 cells were infected with the MP-12 strain of RVFV (MOI: 3) and stained after 3 h using MitoSox stain and DAPI. The stained cells were visualized by confocal microscopy. (B) HepG2 cells infected with MP-12 (MOI: 3) were lysed at 24 h post infection and total protein lysate obtained. Lysates from uninfected cells were obtained as controls and both lysates were resolved by SDS-PAGE and western blots, carried out using anti-RVFV and <t>anti-β-actin</t> <t>antibodies.</t>
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Fig. 1. Increased ROS in <t>RVFV</t> infected HepG2 cells. (A) HepG2 cells were infected with the MP-12 strain of RVFV (MOI: 3) and stained after 3 h using MitoSox stain and DAPI. The stained cells were visualized by confocal microscopy. (B) HepG2 cells infected with MP-12 (MOI: 3) were lysed at 24 h post infection and total protein lysate obtained. Lysates from uninfected cells were obtained as controls and both lysates were resolved by SDS-PAGE and western blots, carried out using anti-RVFV and <t>anti-β-actin</t> <t>antibodies.</t>
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Fig. 1. Increased ROS in <t>RVFV</t> infected HepG2 cells. (A) HepG2 cells were infected with the MP-12 strain of RVFV (MOI: 3) and stained after 3 h using MitoSox stain and DAPI. The stained cells were visualized by confocal microscopy. (B) HepG2 cells infected with MP-12 (MOI: 3) were lysed at 24 h post infection and total protein lysate obtained. Lysates from uninfected cells were obtained as controls and both lysates were resolved by SDS-PAGE and western blots, carried out using anti-RVFV and <t>anti-β-actin</t> <t>antibodies.</t>
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Fig. 1. Increased ROS in <t>RVFV</t> infected HepG2 cells. (A) HepG2 cells were infected with the MP-12 strain of RVFV (MOI: 3) and stained after 3 h using MitoSox stain and DAPI. The stained cells were visualized by confocal microscopy. (B) HepG2 cells infected with MP-12 (MOI: 3) were lysed at 24 h post infection and total protein lysate obtained. Lysates from uninfected cells were obtained as controls and both lysates were resolved by SDS-PAGE and western blots, carried out using anti-RVFV and <t>anti-β-actin</t> <t>antibodies.</t>
Anti Gh Igy Antibody, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ProSci Incorporated envelope protein
Fig. 1. Increased ROS in <t>RVFV</t> infected HepG2 cells. (A) HepG2 cells were infected with the MP-12 strain of RVFV (MOI: 3) and stained after 3 h using MitoSox stain and DAPI. The stained cells were visualized by confocal microscopy. (B) HepG2 cells infected with MP-12 (MOI: 3) were lysed at 24 h post infection and total protein lysate obtained. Lysates from uninfected cells were obtained as controls and both lysates were resolved by SDS-PAGE and western blots, carried out using anti-RVFV and <t>anti-β-actin</t> <t>antibodies.</t>
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Image Search Results


Fig. 1. Increased ROS in RVFV infected HepG2 cells. (A) HepG2 cells were infected with the MP-12 strain of RVFV (MOI: 3) and stained after 3 h using MitoSox stain and DAPI. The stained cells were visualized by confocal microscopy. (B) HepG2 cells infected with MP-12 (MOI: 3) were lysed at 24 h post infection and total protein lysate obtained. Lysates from uninfected cells were obtained as controls and both lysates were resolved by SDS-PAGE and western blots, carried out using anti-RVFV and anti-β-actin antibodies.

Journal: Virology

Article Title: Reactive oxygen species activate NFκB (p65) and p53 and induce apoptosis in RVFV infected liver cells.

doi: 10.1016/j.virol.2013.11.023

Figure Lengend Snippet: Fig. 1. Increased ROS in RVFV infected HepG2 cells. (A) HepG2 cells were infected with the MP-12 strain of RVFV (MOI: 3) and stained after 3 h using MitoSox stain and DAPI. The stained cells were visualized by confocal microscopy. (B) HepG2 cells infected with MP-12 (MOI: 3) were lysed at 24 h post infection and total protein lysate obtained. Lysates from uninfected cells were obtained as controls and both lysates were resolved by SDS-PAGE and western blots, carried out using anti-RVFV and anti-β-actin antibodies.

Article Snippet: Primary antibodies to RVFV (ProSci, Cat# 4519), total p65 (Santa Cruz Biotechnology, Inc. Cat# sc7151), phospho-p65 (ser536) (Santacruz biotechnology, Inc., Cat# 33020), total p53 (Cell Signaling, Cat# 9282S), phospho-p53 (Ser15) (Cell Signaling, Cat# 9284S), Cytochrome C (Abcam, Cat# ab13575) and HRP conjugated actin (Abcam, Cat# ab49900) were used according to manufacturer’s instructions and the blots were incubated overnight at 4 1C.

Techniques: Infection, Staining, Confocal Microscopy, SDS Page, Western Blot