Journal: Antimicrobial Resistance and Infection Control

Article Title: Molecular epidemiological analysis and risk factors for acquisition of carbapenemase-producing Enterobacter cloacae complex in a Japanese university hospital

doi: 10.1186/s13756-019-0578-3

Figure Lengend Snippet: Annual incidence of carbapenem-resistant Enterobacter cloacae complex cases from April 2012 to March 2016 in Nagoya University Hospital. Abbreviations: CPEC, carbapenemase-producing Enterobacter cloacae complex

Article Snippet: DNA was isolated with an UltraClean Microbial DNA isolation kit (MoBio, San Diego, CA, USA) and used for experiments with the Enterobacter spp. fingerprinting kit (bioMerieux Japan, Tokyo, Japan) per the manufacturer’s procedure.

Techniques:

Journal: Antimicrobial Resistance and Infection Control

Article Title: Molecular epidemiological analysis and risk factors for acquisition of carbapenemase-producing Enterobacter cloacae complex in a Japanese university hospital

doi: 10.1186/s13756-019-0578-3

Figure Lengend Snippet: Baseline characteristics and health care exposure of carbapenem-resistant Enterobacter cloacae complex cases

Article Snippet: DNA was isolated with an UltraClean Microbial DNA isolation kit (MoBio, San Diego, CA, USA) and used for experiments with the Enterobacter spp. fingerprinting kit (bioMerieux Japan, Tokyo, Japan) per the manufacturer’s procedure.

Techniques:

Journal: Antimicrobial Resistance and Infection Control

Article Title: Molecular epidemiological analysis and risk factors for acquisition of carbapenemase-producing Enterobacter cloacae complex in a Japanese university hospital

doi: 10.1186/s13756-019-0578-3

Figure Lengend Snippet: Culture source, clinical diagnosis, and outcome among carbapenem-resistant Enterobacter cloacae complex cases

Article Snippet: DNA was isolated with an UltraClean Microbial DNA isolation kit (MoBio, San Diego, CA, USA) and used for experiments with the Enterobacter spp. fingerprinting kit (bioMerieux Japan, Tokyo, Japan) per the manufacturer’s procedure.

Techniques: Infection

Journal: Antimicrobial Resistance and Infection Control

Article Title: Molecular epidemiological analysis and risk factors for acquisition of carbapenemase-producing Enterobacter cloacae complex in a Japanese university hospital

doi: 10.1186/s13756-019-0578-3

Figure Lengend Snippet: Phylogenetic tree based on repetitive extragenic palindromic (REP)-PCR of carbapenem-resistant Enterobacter cloacae complex with hsp60 -based genetic cluster, subspecies, sequence type (ST) classified by multilocus sequence typing and identification of the carbapenemase gene. Genetic relatedness was determined by a similarity cut-off > 95%. a Species and subspecies were determined with reference to the report by Chavda et al. b Newly registered in this study

Article Snippet: DNA was isolated with an UltraClean Microbial DNA isolation kit (MoBio, San Diego, CA, USA) and used for experiments with the Enterobacter spp. fingerprinting kit (bioMerieux Japan, Tokyo, Japan) per the manufacturer’s procedure.

Techniques: Sequencing

Journal: Antimicrobial Resistance and Infection Control

Article Title: Molecular epidemiological analysis and risk factors for acquisition of carbapenemase-producing Enterobacter cloacae complex in a Japanese university hospital

doi: 10.1186/s13756-019-0578-3

Figure Lengend Snippet: Linear comparison of bla IMP-1 -carrying plasmid sequences from Enterobacter cloacae complex strains was performed with BLAST and visualized with Easyfig ( http://mjsull.github.io/Easyfig/ ). Red, yellow, green, and blue arrows indicate the bla IMP-1 gene, other antimicrobial resistant genes (ARG), type IV secretion system (T4SS)-associated genes for conjugation, and mobile gene elements (MGE) detected from CDS annotations, respectively. Structural features of a class 1 integron containing bla IMP-1 are shown, and the structures are almost identical in six plasmids, except for pNUH14_ECL028_1

Article Snippet: DNA was isolated with an UltraClean Microbial DNA isolation kit (MoBio, San Diego, CA, USA) and used for experiments with the Enterobacter spp. fingerprinting kit (bioMerieux Japan, Tokyo, Japan) per the manufacturer’s procedure.

Techniques: Plasmid Preparation, Conjugation Assay