Journal: Frontiers in Microbiology

Article Title: Designing Synergistic Biostimulants Formulation Containing Autochthonous Phosphate-Solubilizing Bacteria for Sustainable Wheat Production

doi: 10.3389/fmicb.2022.889073

Figure Lengend Snippet: Re-isolation and detection of inoculated P-solubilizing bacteria. The CLSM of wheat roots at 35 days post inoculation of P-solubilizing bacteria in the pot experiment under net house conditions. The FLUOS-labeled oligonucleotide probes showed green fluorescent signals for total bacterial population and Cy3-labeled oligonucleotide probes showed red fluorescent signals for inoculated Ochrobactrum sp. SSR. (A) Control treatments without inoculation and from the PSB inoculated pots with carrier materials (B) Biochar, (C) CM, (D) FM, and (E) HA after hybridizing with fluorescently labeled probes. The plant growth promoting traits of re-isolated PSB strains for their confirmation in comparison to pure PSB (F) . The plates depicting the re-isolated colonies of SSR, ZW32, and ZW9 from BC (G) , CM (H) , FM (I) and HA (J) in inoculated treatments. Enterobacter spp. ZW9, ZW32, Ochrobactrum sp. SSR. B, bacteria; RC, root cells.

Article Snippet: These PSB are non-pathogenic, compatible with each other and were also submitted to DSMZ ( https://www.dsmz.de/ ) culture collection under the accession numbers DSM 109610 and DSM 109592 , DSM 109593 for Ochrobactrum sp. SSR, Enterobacter sp. ZW9 and Enterobacter sp. ZW32, respectively.

Techniques: Isolation, Labeling

Journal:

Article Title: Specific Distribution within the Enterobacter cloacae Complex of Strains Isolated from Infected Orthopedic Implants ▿

doi: 10.1128/JCM.00290-09

Figure Lengend Snippet: Neighbor-joining unrooted tree resulting from analysis of the hsp60 gene sequences of 73 clinical strains and previously reported sequences. Isolation site of clinical strains: P, infected orthopedic implant; B, blood; J, joint or bone, in the absence of implanted material; G, gastrointestinal tract; R, respiratory tract; S, skin and soft tissue; U, urine; K, intravascular catheter. For the previously described strains, type strains are indicated (8, 10-12). Strains labeled EN were reported previously (8) and correspond to sequences with GenBank accession numbers AJ417125, AJ417127, AJ543819, AJ567887, AJ543876, AJ543894, AJ567893, AJ543787, AJ543803, AJ543806, AJ543829, AJ543864, AJ543882, AJ543804, AJ543789, AJ543781, AJ543784, AJ567846, AJ543776, AJ543808, AJ543877, AJ543807, AJ543866, AJ543775, AJ543878, AJ543881, AJ543820, AJ567878, AJ567885, AJ543857, AJ543831, AJ543821, AJ543816, AJ543798, AJ567847, AJ543777, AJ543861, AJ543768, AJ543855, AJ543870, AJ567881, and AJ543837. EH, E. hormaechei; EC, E. cloacae. The bar indicates the number of substitutions per site. Genetic clusters are numbered according to the description provided previously (8).

Article Snippet: The sequences of the following type strains were retrieved from the GenBank database (the information in parentheses is the strain designation, GenBank accession number): E. asburiae (ATCC 35953, AJ417141 ), E. kobei (ATCC BAA260, AJ567899 ), E. cloacae subsp. dissolvens (ATCC 23373, AJ417143 ) ( 9 ), E. ludwigii (EN-119, AJ417114 ) ( 11 ), E. hormaechei subsp. oharae (EN-314, AJ543782 ), E. hormaechei subsp. hormaechei (ATCC 49162, AJ417108 ), E. hormaechei subsp. steigerwaltii (CIP108489, AJ543908 ) ( 10 ), E. nimipressuralis (ATCC 9912, AJ567900 ), E. cancerogenus (ATCC 33241, AJ567895 ), E. amnigenus (ATCC 3072, AJ567894 ), E. cowanii (ATCC 107300T, AJ567896 ), E. gergoviae (ATCC 33028, AJ567897 ), E. pyrinus (ATCC 49851, AJ567901 ), C. sakazaki (ATCC 29544, AJ567902 ) ( 12 ), and E. aerogenes ( AB008141 ).

Techniques: Isolation, Infection, Labeling

Journal:

Article Title: Specific Distribution within the Enterobacter cloacae Complex of Strains Isolated from Infected Orthopedic Implants ▿

doi: 10.1128/JCM.00290-09

Figure Lengend Snippet: Distribution of clinical strains within the genetic clusters of the E. cloacae complex. All strains could be assigned to one of the previously reported hsp60 gene sequencing-based genetic clusters of the E. cloacae complex. (A) Strains isolated from implanted orthopedic material at different anatomical sites (n = 21). Hip-associated strains exclusively belonged to the E. hormaechei species (clusters VI and VIII). (B) Randomly selected clinical strains of diverse anatomical origins (n = 52). S, skin and soft tissue; R, upper and lower respiratory tract; U, urine; J, joint or bone, in the absence of infected material; K, intravenous catheter; B, blood; G, gastrointestinal tract. Irrespective of the site if isolation, cluster III accounted for 42% of the control isolates.

Article Snippet: The sequences of the following type strains were retrieved from the GenBank database (the information in parentheses is the strain designation, GenBank accession number): E. asburiae (ATCC 35953, AJ417141 ), E. kobei (ATCC BAA260, AJ567899 ), E. cloacae subsp. dissolvens (ATCC 23373, AJ417143 ) ( 9 ), E. ludwigii (EN-119, AJ417114 ) ( 11 ), E. hormaechei subsp. oharae (EN-314, AJ543782 ), E. hormaechei subsp. hormaechei (ATCC 49162, AJ417108 ), E. hormaechei subsp. steigerwaltii (CIP108489, AJ543908 ) ( 10 ), E. nimipressuralis (ATCC 9912, AJ567900 ), E. cancerogenus (ATCC 33241, AJ567895 ), E. amnigenus (ATCC 3072, AJ567894 ), E. cowanii (ATCC 107300T, AJ567896 ), E. gergoviae (ATCC 33028, AJ567897 ), E. pyrinus (ATCC 49851, AJ567901 ), C. sakazaki (ATCC 29544, AJ567902 ) ( 12 ), and E. aerogenes ( AB008141 ).

Techniques: Sequencing, Isolation, Infection, Control