Journal: Journal of pharmacological sciences

Article Title: Aggravation of cold-restraint stress-induced gastric lesions in adjuvant arthritic rats: pathogenic role of inducible and endothelial nitric oxide.

doi: 10.1254/jphs.09203fp

Figure Lengend Snippet: Fig. 5. Immunohistochemical study for eNOS and iNOS in the gastric mucosa of normal and arthritic rats. The experiment was performed 14 days after the injection of FCA. eNOS and iNOS proteins were stained by Alexa fluor 488 (green) and Alexa Fluor 546 (red), respectively. Immunofluorescence was observed using a confocal laser scanning microscope (LSM-510, magnification: ×200). A: eNOS in normal rats, B: eNOS in arthritic rats, C: iNOS in normal rats, C: iNOS in arthritic rats.

Article Snippet: For the fluorescent immunohistochemical study of eNOS and iNOS, the slides were incubated with rabbit polyclonal anti-human eNOS (NOS 3) antibody (Santa Cruz Biotechnology) or rabbit polyclonal mouse iNOS (NOS 2) antibody (BD Transduction Laboratory) and then treated with Alexa Fluor 488–labeled donkey polyclonal anti-rabbit IgG antibody (Molecular Probes, Eugene, OR, USA) or Alexa Fluor 546–labeled donkey polyclonal anti-rabbit IgG antibody (Molecular Probes), respectively.

Techniques: Immunohistochemical staining, Injection, Staining, Immunofluorescence, Laser-Scanning Microscopy

Journal: Hypertension

Article Title: Renal Collecting Duct NOS1 Maintains Fluid–Electrolyte Homeostasis and Blood Pressure

doi: 10.1161/hypertensionaha.113.01291

Figure Lengend Snippet: Figure 1. NOS1 splice variant expression of the inner medulla (IM) and cerebellum (CB) from wild-type mouse (WT) and NOS1αKO mouse as determined by Western blotting with anti- NOS1 (C terminus). A, NOS1α is expressed in the cerebellum of the WT mouse but not in the cerebellum of NOS1αKO mice. NOS1β is expressed in the IM of WT and NOS1αKO mice. The molecular weight marker (MW) was run on the same gel, but it was noncontiguous. B, NOS1β and NOS3 coexpressed in the IM, and NOS1α and NOS3 coexpressed in the cerebellum of the WT mouse. The molecular weight marker (MW) was run on the same gel, but it was noncontiguous. C, Collecting duct expression of NOS1β in the WT and NOS1αKO mice.

Article Snippet: IHC - immunohistochemistry Antibody Sequence Amino Acids Host Application Concentration Company Location NOS1 rat C-terminus rabbit polyclonal IB 20 μg/10ml Santa Cruz Santa Cruz, CA NOS1 human 1414-1434 rabbit polyclonal IB, IHC 1.4 μg Biomol Plymouth Meeting, PA NOS3 human 1025-1203 Mouse monoclonal IB 12.5 μg/ 10ml BD Biosciences San Jose, CA CD3-ε mouse C-terminus goat polyclonal IHC 0.2 μg Santa Cruz Santa Cruz, CA F4/80 mouse ? rat monoclonal IHC 0.1 μg AbD Serotech Raleigh, NC 3 NOS1!"

Techniques: Variant Assay, Expressing, Western Blot, Molecular Weight, Marker

Journal: Journal of the American Heart Association

Article Title: Interleukin‐1β Disruption Protects Male Mice From Heart Failure With Preserved Ejection Fraction Pathogenesis

doi: 10.1161/jaha.122.029668

Figure Lengend Snippet: Figure 3. Western blot analysis and nitrate/nitrite levels. Western blot analysis and cumulative data for phosphorylated endothelial nitric-oxide synthase (P-eNOS) in mesenteric resistance arteries (MRAs) isolated from C57/BL6J (C57), C57/BL6J+high-fat diet (HFD)+N[w]-nitro-l-arginine methyl ester (L-NAME), interleukin- 1β−/− (IL-1β−/−), and IL-1β−/−+HFD+L-NAME male mice (n=5) (A) and nitrate/nitrite level in the urine (B) from all groups of mice. One-way ANOVA followed by Tukey’s multiple comparisons post hoc test was applied for (A and B). *P<0.05, ****P<0.0001 for C57BL/6J vs C57/ Bl6+HFD+L-NAME; (n=5). ns indicates not significant; and T-eNOS, total nitric oxide synthase.

Article Snippet: We used specific antibodies against endothelial nitric oxide synthase (eNOS; Cell Signaling, D9A5L rabbit monoclonal antibodies; number 32027), phospho- eNOS (Ser1177; Cell D ow nloaded from http://ahajournals.org by on January 26, 2024 Signaling [C9C3] rabbit monoclonal antibodies, 9570), iNOS (Proteintech, number 18985- 1- AP), anti- COX2 (Cayman; aa 584– 598, number 160126), NLR family pyrin domain containing 3/Nacht leucine- rich repeat protein 3 (Novus Biotech; number NBP2- 12446), X- box binding protein 1 (Abcam; number ab238456), anti– CCAAT/enhancer- binding protein (Cell Signaling; L63F7 mouse monoclonal antibodies number 2895), IRE1 (Abcam; number ab37073, lot GR3354835- 4), activating transcription factor 6 (Abcam; number ab37149, lot GR3353621- 3), and beta- actin (Santa Cruz; number SC47778).

Techniques: Western Blot, Isolation

Journal: Nutrients

Article Title: Analysis of the Combined Effects of a Novel Combination of Hypersmin, Pumpkin Seed and Amaranthus Extracts in an In Vitro Model of Chronic Venous Insufficiency.

doi: 10.3390/nu17111807

Figure Lengend Snippet: Figure 3. Vascular tone analysis on in vitro model of vein after intestinal passage. In (A) NO production; in (B) eNOS levels; in (C) endothelin-1 levels. From (A) to (C) all the data are obtained with specific ELISA kits; * p < 0.05 vs. Control; α p < 0.05 vs. single agents; β p < 0.05 vs. Commercial product; γ p < 0.05 vs. KCl.

Article Snippet: The eNOS concentration was measured following the manufacturer’s instructions for the DuoSet ELISA kit for Human eNOS (R&D Systems, Minneapolis, MN, USA) in HUVEC cells [39].

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Control

Journal: Al-Rafidain Journal of Medical Sciences ( ISSN 2789-3219 )

Article Title: Effect of Nicorandil on Endothelial Markers and Tissue Remodeling in Pulmonary Arterial Hypertension Model of Male Rats

doi: 10.54133/ajms.v5i1s.334

Figure Lengend Snippet: Figure 3: Number of folds expressed as percentages for endothelial nitric oxide synthase (eNOS) expression in studied groups. Statistically significant at p<0.05 (ANOVA followed Tukey test).

Article Snippet: The heated samples were centrifuged at 4 °C for 2 min at 12000 rpm, and the upper layer was equally loaded into the wheels of 8% gel together with the pre-stained protein [19]. (endothelial nitric oxide synthase (eNOS) monoclonal antibody E-AB-22088) and BCA kit E-BC-K318-M) purchased from Elabscience, the procedure was followed according to the manufacturer’s guidelines.

Techniques: Expressing