Journal: Journal of Cellular and Molecular Medicine

Article Title: E10A, an adenovirus carrying human endostatin gene, in combination with docetaxel treatment inhibits prostate cancer growth and metastases

doi: 10.1111/j.1582-4934.2008.00548.x

Figure Lengend Snippet: (A) LYVE-1-positive lymphatic vessels in orthotopic tumours received the different treatment. Representative images were shown (×200). (B) Serum endostatin levels in the mice with orthotopic tumours. After systemic delivery of E10A, serum endostatin levels were determined by ELISA. Serum endostatin levels in mice treated with Ad-LacZ were used as control. (C) Serum VEGF levels in different groups of mice. Mice were killed after 4 weeks of treatment and serum were collected for determination of VEGF levels by ELISA. Columns, mean ± S.D. * P < 0.05, compared with Ad-LacZ control.

Article Snippet: The supernatants of PC-3 cells after 72 hrs of infection with E10A or Ad-LacZ at MOI of 100 were harvested as conditioned medium (CM) and evaluated endostatin protein using a human endostatin ELISA kit (R&D systems, Minneapolis, MN, USA) according to the recommendation of the manufacturer.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Tissue Engineering. Part A

Article Title: Engineering Endostatin-Producing Cartilaginous Constructs for Cartilage Repair Using Nonviral Transfection of Chondrocyte-Seeded and Mesenchymal-Stem-Cell-Seeded Collagen Scaffolds

doi: 10.1089/ten.tea.2009.0771

Figure Lengend Snippet: Endostatin in the medium of cell-seeded, lipoplex-supplemented collagen constructs and control constructs. (a) Mesenchymal-stem-cell (MSC)-seeded type I/III constructs cultured in chondrogenic medium (CM) and nonchondrogenic medium (NCM) (n = 3 for first 6 days of lipoplex-supplemented scaffolds; n = 2 for all others). (b) Modification of the scaffold via crosslinking none, half, or all of the lipoplexes (n = 6), in MSC-seeded type I/III constructs cultured in CM. (c) MSCs cultured at standard and low oxygen in type I/III and type II collagen scaffolds in CM (n = 3). (d) Chondrocytes cultured at standard and low oxygen in type I/III and type II collagen scaffolds in CM (n = 3).

Article Snippet: Endostatin detection in the medium Endostatin protein in the culture medium was measured using a sandwich enzyme-linked immunosorbent assay (ELISA) kit for human endostatin protein (R&D Systems) following the manufacturer's instructions.

Techniques: Construct, Control, Cell Culture, Modification

Journal: Tissue Engineering. Part A

Article Title: Engineering Endostatin-Producing Cartilaginous Constructs for Cartilage Repair Using Nonviral Transfection of Chondrocyte-Seeded and Mesenchymal-Stem-Cell-Seeded Collagen Scaffolds

doi: 10.1089/ten.tea.2009.0771

Figure Lengend Snippet: Micrographs of histological sections of chondrocyte- and MSC-seeded constructs cultured in CM and NCM at standard oxygen. (a) Endostatin immunohistochemistry of MSC-seeded CI scaffold supplemented with 20 μg pEndo after 6 days of culture in NCM (red chromogen [arrows] indicates positive stain). Scale bar, 50 μm, 40 × magnification. (b) Hematoxylin and eosin stain of the interior of MSC-seeded CI scaffold supplemented with 4 μg pEndo after 22 days of culture in CM. Scale bar, 50 μm, 40 × magnification. Inset shows the section at lower magnification, scale bar, 100 μm, 10 × magnification. (c) Hematoxylin and eosin stain of the surface of MSC-seeded CI scaffold supplemented with 4 μg pEndo after 22 days of culture in CM. Scale bar, 100 μm, 10 × magnification. (d) Safranin-O staining of glycosaminoglycan (GAG) for MSC-seeded CI scaffold supplemented with 4 μg pEndo after 22 days of culture in CM (red chromogen indicates sulfated GAGs). Scale bar, 200 μm, 10× magnification. (e) Type II collagen immunohistochemistry of MSC-seeded CI scaffold supplemented with 4 μg pEndo after 22 days of culture in CM (red indicates positive stain). Scale bar, 200 μm, 10 × magnification. (f) Safranin-O staining of GAG for chondrocyte-seeded CI scaffold supplemented with 20 μg pEndo after 20 days of culture in CM (red chromogen indicates sulfated GAGs). Scale bar, 50 μm, 40 × magnification. Inset shows the section at lower magnification, scale bar, 500 μm, 4 × magnification. (g) Type II collagen immunohistochemistry of chondrocyte-seeded CI scaffold supplemented with 20 μg pEndo after 20 days of culture in CM (brownish-red chromogen indicates positive stain). Scale bar, 500 μm, 4 × magnification. (h) Masson's trichrome staining of chondrocyte-seeded CI scaffold supplemented with 20 μg pEndo after 20 days of culture in CM (blue chromogen indicates collagen, red chromogen indicates cytoplasm, and black chromogen indicates nuclei). Scale bar, 500 μm, 4 × magnification. Color images available online at www.liebertonline.com/ten.

Article Snippet: Endostatin detection in the medium Endostatin protein in the culture medium was measured using a sandwich enzyme-linked immunosorbent assay (ELISA) kit for human endostatin protein (R&D Systems) following the manufacturer's instructions.

Techniques: Construct, Cell Culture, Immunohistochemistry, Staining, H&E Stain

Journal: BMC Cancer

Article Title: Endostar acts as a pneumonitis protectant in patients with locally advanced non-small cell lung cancer receiving concurrent chemoradiotherapy

doi: 10.1186/s12885-024-12001-6

Figure Lengend Snippet: Baseline and clinical characteristics of patients and their correlation with Endostar

Article Snippet: Tanabe et al [ ] found that endostatin suppressed peritoneal fibrosis by significantly reducing vascular endothelial growth factor A (VEGF-A), alpha-smooth muscle actin (α-SMA), and the pro-fibrotic factor TGF-β1 in a mouse model. Another investigation revealed that Endostar alleviated radiation-induced pulmonary alveolitis, pulmonary edema, fibrosis, and TGF-β1 release in mice compared to radiation-exposed mice treated without Endostar, suggesting that Endostar can reduce radiation-induced pulmonary lesions [ ].

Techniques:

Journal: BMC Cancer

Article Title: Endostar acts as a pneumonitis protectant in patients with locally advanced non-small cell lung cancer receiving concurrent chemoradiotherapy

doi: 10.1186/s12885-024-12001-6

Figure Lengend Snippet: The difference in the incidence of ≥ grade 2 RP at different subgroups

Article Snippet: Tanabe et al [ ] found that endostatin suppressed peritoneal fibrosis by significantly reducing vascular endothelial growth factor A (VEGF-A), alpha-smooth muscle actin (α-SMA), and the pro-fibrotic factor TGF-β1 in a mouse model. Another investigation revealed that Endostar alleviated radiation-induced pulmonary alveolitis, pulmonary edema, fibrosis, and TGF-β1 release in mice compared to radiation-exposed mice treated without Endostar, suggesting that Endostar can reduce radiation-induced pulmonary lesions [ ].

Techniques:

Journal: BMC Cancer

Article Title: Endostar acts as a pneumonitis protectant in patients with locally advanced non-small cell lung cancer receiving concurrent chemoradiotherapy

doi: 10.1186/s12885-024-12001-6

Figure Lengend Snippet: The efficacy of endostar with NSCLC in previously reported studies

Article Snippet: Tanabe et al [ ] found that endostatin suppressed peritoneal fibrosis by significantly reducing vascular endothelial growth factor A (VEGF-A), alpha-smooth muscle actin (α-SMA), and the pro-fibrotic factor TGF-β1 in a mouse model. Another investigation revealed that Endostar alleviated radiation-induced pulmonary alveolitis, pulmonary edema, fibrosis, and TGF-β1 release in mice compared to radiation-exposed mice treated without Endostar, suggesting that Endostar can reduce radiation-induced pulmonary lesions [ ].

Techniques: Control

Journal: BMC Cancer

Article Title: Endostar acts as a pneumonitis protectant in patients with locally advanced non-small cell lung cancer receiving concurrent chemoradiotherapy

doi: 10.1186/s12885-024-12001-6

Figure Lengend Snippet: Clinical trials about Endostar combined with ICIs in NSCLC

Article Snippet: Tanabe et al [ ] found that endostatin suppressed peritoneal fibrosis by significantly reducing vascular endothelial growth factor A (VEGF-A), alpha-smooth muscle actin (α-SMA), and the pro-fibrotic factor TGF-β1 in a mouse model. Another investigation revealed that Endostar alleviated radiation-induced pulmonary alveolitis, pulmonary edema, fibrosis, and TGF-β1 release in mice compared to radiation-exposed mice treated without Endostar, suggesting that Endostar can reduce radiation-induced pulmonary lesions [ ].

Techniques: Clinical Proteomics

Journal: British Journal of Pharmacology

Article Title: PEP06 polypeptide 30 exerts antitumour effect in colorectal carcinoma via inhibiting epithelial–mesenchymal transition

doi: 10.1111/bph.14352

Figure Lengend Snippet: PEP06 down‐regulated the expression of miR‐146b‐5p by forming high‐affinity interactions with αvβ3 integrin in CRC cells. (A) Heat map of microRNA microarray analysis showing the alterations in the expression of miRNAs in SW620 and HCT116 cells exposed to 200 μg·mL−1 PEP06 for 24 h relative to control cells. SW620C1, SW620C2, HCT116C1 and HCT116C2 represent control samples in duplicate, and SW620 + 1, SW620 + 2, HCT116 + 1 and HCT116 + 2 represent PEP06‐treated samples in duplicate. (B, C) qRT‐PCR verification of miR‐146b‐5p down‐regulation in SW620 and HCT116 cells treated with varying concentrations of PEP06. Data (mean ± SEM) were from eight independent experiments. * P < 0.05 by one‐way ANOVA, Dunnett's test: compared with Ctl. (D, E) qRT‐PCR verification of miR‐146b‐5p down‐regulation in SW620 and HCT116 cells treated with PEP06 compared with 24aa, Endostar and cilengitide. Data (mean ± SEM) were from five independent experiments. * P < 0.05 by one‐way ANOVA, Dunnett's test: compared with Ctl. (F) Sensorgrams of αvβ3 integrin (1–32 nM) binding to immobilized PEP06 (50 nM) and K D values calculated. The kinetic parameters were measured by Biacore evaluation software. (G) SPR sensorgrams showing no binding of αvβ3 integrin (1–32 nM) to immobilized 24aa (50 nM).

Article Snippet: A similar positive peptide drug ENDOSTAR ® was purchased from Simcere Pharmaceutical Group (Shandong, China).

Techniques: Expressing, Microarray, Control, Quantitative RT-PCR, Binding Assay, Software

Journal: Experimental and Therapeutic Medicine

Article Title: Continuous administration of recombinant human endostatin (Endostar): A pre-clinical safety study

doi: 10.3892/etm.2012.534

Figure Lengend Snippet: Body weights (g) of the mice in the different treatment groups.

Article Snippet: The assay had been validated by the Simcere Pharmaceutical Group using standard Endostar with a detection limit of 12.5–400 ng/ml.

Techniques: Saline

Journal: Experimental and Therapeutic Medicine

Article Title: Continuous administration of recombinant human endostatin (Endostar): A pre-clinical safety study

doi: 10.3892/etm.2012.534

Figure Lengend Snippet: Histologic findings in heart, kidney and lung tissues following Endostar administration (H&E staining, ×200). (A–C) Tissues of (A) heart, (B) kidney and (C) lung in the saline group. (D–F) Tissues of (D) heart, (E) kidney and (F) lung in the intermittent administration group. (G–I) Tissues of (G) heart, (H) kidney and (I) lung in the continuous administration group. H&E, hematoxylin and eosin.

Article Snippet: The assay had been validated by the Simcere Pharmaceutical Group using standard Endostar with a detection limit of 12.5–400 ng/ml.

Techniques: Staining, Saline

Journal: Experimental and Therapeutic Medicine

Article Title: Continuous administration of recombinant human endostatin (Endostar): A pre-clinical safety study

doi: 10.3892/etm.2012.534

Figure Lengend Snippet: CD146-labeled microvessels in heart, kidney and lung tissues following Endostar administration (x200). (A–C) CD146-labeled microvessels in the tissues of (A) heart, (B) kidney and (C) lung in the saline group. (D–F) CD146-labeled microvessels in the tissues of (D) heart, (E) kidney and (F) lung in the intermittent administration group. (G–I) CD146-labeled microvessels in the tissues of (G) heart, (H) kidney and (I) lung in the continuous administration group.

Article Snippet: The assay had been validated by the Simcere Pharmaceutical Group using standard Endostar with a detection limit of 12.5–400 ng/ml.

Techniques: Labeling, Saline