endonucleases Search Results


neb endo iv  (New England Biolabs)
95
New England Biolabs neb endo iv
Neb Endo Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hl dsdnase  (ArcticZymes)
95
ArcticZymes hl dsdnase
Hl Dsdnase, supplied by ArcticZymes, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t7 endonuclease  (New England Biolabs)
97
New England Biolabs t7 endonuclease
T7 Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endonuclease v  (New England Biolabs)
97
New England Biolabs endonuclease v
a Schematic diagram of the color-switching construct of the rd12 reporter-cell line. An intervening sequence from the Rpe65 - rd12 genomic sequence containing the mutation (c.130 C  >  T; p.R44X) was inserted into a gene expressing both mCherry and eGFP. Expression of eGFP in the reporter cells is prevented by a STOP codon, which can be restored via proper action of ABE. b Representative fluorescence-microscopic images of rd12 -reporter cells 48 h after adding the RNP-CBB complex. Scale bar, 400 μm. c , d Quantitative assessment of ABE-RNP delivery into rd12 -reporter cells, mediated by lipidoid complexes of ABE8e with CBB compounds ( c ) by flow cytometry; or CBBZ compounds ( d ) by fluorescence-microscopic imaging only. [ABE8e]/[CBB(Z)] = 1:8. A, ABE8e-RNP only. NE, no enzyme. Quantification of ABE8e RNP delivery mediated by Lipofectamine 3000 (LF) is included as a positive control. The ratio (eGFP/mCherry) reflects the relative editing efficiency. The CBB compounds were considered positive hits if the ratio of green-to-red (eGFP/mCherry) was more than twice that of A (the dashed line). Data are presented as mean ± SD, n = 4 replicates ( c ) and 3 replicates ( d ). e Schematic diagram of the ABE-activity assay. EndoV = endonuclease V. Blue, SpCas9 PAM; red, target base; orange, bystander base; arrowhead, nick site; FAM, fluorescein. f Representative urea-PAGE-gel image of products of ABE deamination cleaved by EndoV. The gel was imaged with a fluorescein filter. N, no enzyme; P, positive control: 60 bp DNA with inosine in the middle; A, ABE-RNP in TAS; D, ABE-RNP in TAS with 10% DMSO. Numbers indicate molar excess of the CBB compound versus ABE. g Fluorescence quantification of substrate and products of deamination and EndoV cleavage. Two independent samples per data point were assayed in parallel. Source data are provided as a Source Data file.
Endonuclease V, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endonuclease viii  (New England Biolabs)
95
New England Biolabs endonuclease viii
a Schematic diagram of the color-switching construct of the rd12 reporter-cell line. An intervening sequence from the Rpe65 - rd12 genomic sequence containing the mutation (c.130 C  >  T; p.R44X) was inserted into a gene expressing both mCherry and eGFP. Expression of eGFP in the reporter cells is prevented by a STOP codon, which can be restored via proper action of ABE. b Representative fluorescence-microscopic images of rd12 -reporter cells 48 h after adding the RNP-CBB complex. Scale bar, 400 μm. c , d Quantitative assessment of ABE-RNP delivery into rd12 -reporter cells, mediated by lipidoid complexes of ABE8e with CBB compounds ( c ) by flow cytometry; or CBBZ compounds ( d ) by fluorescence-microscopic imaging only. [ABE8e]/[CBB(Z)] = 1:8. A, ABE8e-RNP only. NE, no enzyme. Quantification of ABE8e RNP delivery mediated by Lipofectamine 3000 (LF) is included as a positive control. The ratio (eGFP/mCherry) reflects the relative editing efficiency. The CBB compounds were considered positive hits if the ratio of green-to-red (eGFP/mCherry) was more than twice that of A (the dashed line). Data are presented as mean ± SD, n = 4 replicates ( c ) and 3 replicates ( d ). e Schematic diagram of the ABE-activity assay. EndoV = endonuclease V. Blue, SpCas9 PAM; red, target base; orange, bystander base; arrowhead, nick site; FAM, fluorescein. f Representative urea-PAGE-gel image of products of ABE deamination cleaved by EndoV. The gel was imaged with a fluorescein filter. N, no enzyme; P, positive control: 60 bp DNA with inosine in the middle; A, ABE-RNP in TAS; D, ABE-RNP in TAS with 10% DMSO. Numbers indicate molar excess of the CBB compound versus ABE. g Fluorescence quantification of substrate and products of deamination and EndoV cleavage. Two independent samples per data point were assayed in parallel. Source data are provided as a Source Data file.
Endonuclease Viii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mismatch sensitive endonuclease assay  (New England Biolabs)
94
New England Biolabs mismatch sensitive endonuclease assay
a Schematic diagram of the color-switching construct of the rd12 reporter-cell line. An intervening sequence from the Rpe65 - rd12 genomic sequence containing the mutation (c.130 C  >  T; p.R44X) was inserted into a gene expressing both mCherry and eGFP. Expression of eGFP in the reporter cells is prevented by a STOP codon, which can be restored via proper action of ABE. b Representative fluorescence-microscopic images of rd12 -reporter cells 48 h after adding the RNP-CBB complex. Scale bar, 400 μm. c , d Quantitative assessment of ABE-RNP delivery into rd12 -reporter cells, mediated by lipidoid complexes of ABE8e with CBB compounds ( c ) by flow cytometry; or CBBZ compounds ( d ) by fluorescence-microscopic imaging only. [ABE8e]/[CBB(Z)] = 1:8. A, ABE8e-RNP only. NE, no enzyme. Quantification of ABE8e RNP delivery mediated by Lipofectamine 3000 (LF) is included as a positive control. The ratio (eGFP/mCherry) reflects the relative editing efficiency. The CBB compounds were considered positive hits if the ratio of green-to-red (eGFP/mCherry) was more than twice that of A (the dashed line). Data are presented as mean ± SD, n = 4 replicates ( c ) and 3 replicates ( d ). e Schematic diagram of the ABE-activity assay. EndoV = endonuclease V. Blue, SpCas9 PAM; red, target base; orange, bystander base; arrowhead, nick site; FAM, fluorescein. f Representative urea-PAGE-gel image of products of ABE deamination cleaved by EndoV. The gel was imaged with a fluorescein filter. N, no enzyme; P, positive control: 60 bp DNA with inosine in the middle; A, ABE-RNP in TAS; D, ABE-RNP in TAS with 10% DMSO. Numbers indicate molar excess of the CBB compound versus ABE. g Fluorescence quantification of substrate and products of deamination and EndoV cleavage. Two independent samples per data point were assayed in parallel. Source data are provided as a Source Data file.
Mismatch Sensitive Endonuclease Assay, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tth endoiv  (New England Biolabs)
93
New England Biolabs tth endoiv
a Schematic diagram of the color-switching construct of the rd12 reporter-cell line. An intervening sequence from the Rpe65 - rd12 genomic sequence containing the mutation (c.130 C  >  T; p.R44X) was inserted into a gene expressing both mCherry and eGFP. Expression of eGFP in the reporter cells is prevented by a STOP codon, which can be restored via proper action of ABE. b Representative fluorescence-microscopic images of rd12 -reporter cells 48 h after adding the RNP-CBB complex. Scale bar, 400 μm. c , d Quantitative assessment of ABE-RNP delivery into rd12 -reporter cells, mediated by lipidoid complexes of ABE8e with CBB compounds ( c ) by flow cytometry; or CBBZ compounds ( d ) by fluorescence-microscopic imaging only. [ABE8e]/[CBB(Z)] = 1:8. A, ABE8e-RNP only. NE, no enzyme. Quantification of ABE8e RNP delivery mediated by Lipofectamine 3000 (LF) is included as a positive control. The ratio (eGFP/mCherry) reflects the relative editing efficiency. The CBB compounds were considered positive hits if the ratio of green-to-red (eGFP/mCherry) was more than twice that of A (the dashed line). Data are presented as mean ± SD, n = 4 replicates ( c ) and 3 replicates ( d ). e Schematic diagram of the ABE-activity assay. EndoV = endonuclease V. Blue, SpCas9 PAM; red, target base; orange, bystander base; arrowhead, nick site; FAM, fluorescein. f Representative urea-PAGE-gel image of products of ABE deamination cleaved by EndoV. The gel was imaged with a fluorescein filter. N, no enzyme; P, positive control: 60 bp DNA with inosine in the middle; A, ABE-RNP in TAS; D, ABE-RNP in TAS with 10% DMSO. Numbers indicate molar excess of the CBB compound versus ABE. g Fluorescence quantification of substrate and products of deamination and EndoV cleavage. Two independent samples per data point were assayed in parallel. Source data are provided as a Source Data file.
Tth Endoiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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single end libraries  (New England Biolabs)
99
New England Biolabs single end libraries
a Schematic diagram of the color-switching construct of the rd12 reporter-cell line. An intervening sequence from the Rpe65 - rd12 genomic sequence containing the mutation (c.130 C  >  T; p.R44X) was inserted into a gene expressing both mCherry and eGFP. Expression of eGFP in the reporter cells is prevented by a STOP codon, which can be restored via proper action of ABE. b Representative fluorescence-microscopic images of rd12 -reporter cells 48 h after adding the RNP-CBB complex. Scale bar, 400 μm. c , d Quantitative assessment of ABE-RNP delivery into rd12 -reporter cells, mediated by lipidoid complexes of ABE8e with CBB compounds ( c ) by flow cytometry; or CBBZ compounds ( d ) by fluorescence-microscopic imaging only. [ABE8e]/[CBB(Z)] = 1:8. A, ABE8e-RNP only. NE, no enzyme. Quantification of ABE8e RNP delivery mediated by Lipofectamine 3000 (LF) is included as a positive control. The ratio (eGFP/mCherry) reflects the relative editing efficiency. The CBB compounds were considered positive hits if the ratio of green-to-red (eGFP/mCherry) was more than twice that of A (the dashed line). Data are presented as mean ± SD, n = 4 replicates ( c ) and 3 replicates ( d ). e Schematic diagram of the ABE-activity assay. EndoV = endonuclease V. Blue, SpCas9 PAM; red, target base; orange, bystander base; arrowhead, nick site; FAM, fluorescein. f Representative urea-PAGE-gel image of products of ABE deamination cleaved by EndoV. The gel was imaged with a fluorescein filter. N, no enzyme; P, positive control: 60 bp DNA with inosine in the middle; A, ABE-RNP in TAS; D, ABE-RNP in TAS with 10% DMSO. Numbers indicate molar excess of the CBB compound versus ABE. g Fluorescence quantification of substrate and products of deamination and EndoV cleavage. Two independent samples per data point were assayed in parallel. Source data are provided as a Source Data file.
Single End Libraries, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endoviii cat no m0299s  (New England Biolabs)
95
New England Biolabs endoviii cat no m0299s
a Schematic diagram of the color-switching construct of the rd12 reporter-cell line. An intervening sequence from the Rpe65 - rd12 genomic sequence containing the mutation (c.130 C  >  T; p.R44X) was inserted into a gene expressing both mCherry and eGFP. Expression of eGFP in the reporter cells is prevented by a STOP codon, which can be restored via proper action of ABE. b Representative fluorescence-microscopic images of rd12 -reporter cells 48 h after adding the RNP-CBB complex. Scale bar, 400 μm. c , d Quantitative assessment of ABE-RNP delivery into rd12 -reporter cells, mediated by lipidoid complexes of ABE8e with CBB compounds ( c ) by flow cytometry; or CBBZ compounds ( d ) by fluorescence-microscopic imaging only. [ABE8e]/[CBB(Z)] = 1:8. A, ABE8e-RNP only. NE, no enzyme. Quantification of ABE8e RNP delivery mediated by Lipofectamine 3000 (LF) is included as a positive control. The ratio (eGFP/mCherry) reflects the relative editing efficiency. The CBB compounds were considered positive hits if the ratio of green-to-red (eGFP/mCherry) was more than twice that of A (the dashed line). Data are presented as mean ± SD, n = 4 replicates ( c ) and 3 replicates ( d ). e Schematic diagram of the ABE-activity assay. EndoV = endonuclease V. Blue, SpCas9 PAM; red, target base; orange, bystander base; arrowhead, nick site; FAM, fluorescein. f Representative urea-PAGE-gel image of products of ABE deamination cleaved by EndoV. The gel was imaged with a fluorescein filter. N, no enzyme; P, positive control: 60 bp DNA with inosine in the middle; A, ABE-RNP in TAS; D, ABE-RNP in TAS with 10% DMSO. Numbers indicate molar excess of the CBB compound versus ABE. g Fluorescence quantification of substrate and products of deamination and EndoV cleavage. Two independent samples per data point were assayed in parallel. Source data are provided as a Source Data file.
Endoviii Cat No M0299s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t7ei  (New England Biolabs)
97
New England Biolabs t7ei
a Schematic diagram of the color-switching construct of the rd12 reporter-cell line. An intervening sequence from the Rpe65 - rd12 genomic sequence containing the mutation (c.130 C  >  T; p.R44X) was inserted into a gene expressing both mCherry and eGFP. Expression of eGFP in the reporter cells is prevented by a STOP codon, which can be restored via proper action of ABE. b Representative fluorescence-microscopic images of rd12 -reporter cells 48 h after adding the RNP-CBB complex. Scale bar, 400 μm. c , d Quantitative assessment of ABE-RNP delivery into rd12 -reporter cells, mediated by lipidoid complexes of ABE8e with CBB compounds ( c ) by flow cytometry; or CBBZ compounds ( d ) by fluorescence-microscopic imaging only. [ABE8e]/[CBB(Z)] = 1:8. A, ABE8e-RNP only. NE, no enzyme. Quantification of ABE8e RNP delivery mediated by Lipofectamine 3000 (LF) is included as a positive control. The ratio (eGFP/mCherry) reflects the relative editing efficiency. The CBB compounds were considered positive hits if the ratio of green-to-red (eGFP/mCherry) was more than twice that of A (the dashed line). Data are presented as mean ± SD, n = 4 replicates ( c ) and 3 replicates ( d ). e Schematic diagram of the ABE-activity assay. EndoV = endonuclease V. Blue, SpCas9 PAM; red, target base; orange, bystander base; arrowhead, nick site; FAM, fluorescein. f Representative urea-PAGE-gel image of products of ABE deamination cleaved by EndoV. The gel was imaged with a fluorescein filter. N, no enzyme; P, positive control: 60 bp DNA with inosine in the middle; A, ABE-RNP in TAS; D, ABE-RNP in TAS with 10% DMSO. Numbers indicate molar excess of the CBB compound versus ABE. g Fluorescence quantification of substrate and products of deamination and EndoV cleavage. Two independent samples per data point were assayed in parallel. Source data are provided as a Source Data file.
T7ei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti fen1  (Proteintech)
93
Proteintech anti fen1
a Schematic diagram of the color-switching construct of the rd12 reporter-cell line. An intervening sequence from the Rpe65 - rd12 genomic sequence containing the mutation (c.130 C  >  T; p.R44X) was inserted into a gene expressing both mCherry and eGFP. Expression of eGFP in the reporter cells is prevented by a STOP codon, which can be restored via proper action of ABE. b Representative fluorescence-microscopic images of rd12 -reporter cells 48 h after adding the RNP-CBB complex. Scale bar, 400 μm. c , d Quantitative assessment of ABE-RNP delivery into rd12 -reporter cells, mediated by lipidoid complexes of ABE8e with CBB compounds ( c ) by flow cytometry; or CBBZ compounds ( d ) by fluorescence-microscopic imaging only. [ABE8e]/[CBB(Z)] = 1:8. A, ABE8e-RNP only. NE, no enzyme. Quantification of ABE8e RNP delivery mediated by Lipofectamine 3000 (LF) is included as a positive control. The ratio (eGFP/mCherry) reflects the relative editing efficiency. The CBB compounds were considered positive hits if the ratio of green-to-red (eGFP/mCherry) was more than twice that of A (the dashed line). Data are presented as mean ± SD, n = 4 replicates ( c ) and 3 replicates ( d ). e Schematic diagram of the ABE-activity assay. EndoV = endonuclease V. Blue, SpCas9 PAM; red, target base; orange, bystander base; arrowhead, nick site; FAM, fluorescein. f Representative urea-PAGE-gel image of products of ABE deamination cleaved by EndoV. The gel was imaged with a fluorescein filter. N, no enzyme; P, positive control: 60 bp DNA with inosine in the middle; A, ABE-RNP in TAS; D, ABE-RNP in TAS with 10% DMSO. Numbers indicate molar excess of the CBB compound versus ABE. g Fluorescence quantification of substrate and products of deamination and EndoV cleavage. Two independent samples per data point were assayed in parallel. Source data are provided as a Source Data file.
Anti Fen1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t7 endonuclease assay  (Genecopoeia)
94
Genecopoeia t7 endonuclease assay
a Schematic diagram of the color-switching construct of the rd12 reporter-cell line. An intervening sequence from the Rpe65 - rd12 genomic sequence containing the mutation (c.130 C  >  T; p.R44X) was inserted into a gene expressing both mCherry and eGFP. Expression of eGFP in the reporter cells is prevented by a STOP codon, which can be restored via proper action of ABE. b Representative fluorescence-microscopic images of rd12 -reporter cells 48 h after adding the RNP-CBB complex. Scale bar, 400 μm. c , d Quantitative assessment of ABE-RNP delivery into rd12 -reporter cells, mediated by lipidoid complexes of ABE8e with CBB compounds ( c ) by flow cytometry; or CBBZ compounds ( d ) by fluorescence-microscopic imaging only. [ABE8e]/[CBB(Z)] = 1:8. A, ABE8e-RNP only. NE, no enzyme. Quantification of ABE8e RNP delivery mediated by Lipofectamine 3000 (LF) is included as a positive control. The ratio (eGFP/mCherry) reflects the relative editing efficiency. The CBB compounds were considered positive hits if the ratio of green-to-red (eGFP/mCherry) was more than twice that of A (the dashed line). Data are presented as mean ± SD, n = 4 replicates ( c ) and 3 replicates ( d ). e Schematic diagram of the ABE-activity assay. EndoV = endonuclease V. Blue, SpCas9 PAM; red, target base; orange, bystander base; arrowhead, nick site; FAM, fluorescein. f Representative urea-PAGE-gel image of products of ABE deamination cleaved by EndoV. The gel was imaged with a fluorescein filter. N, no enzyme; P, positive control: 60 bp DNA with inosine in the middle; A, ABE-RNP in TAS; D, ABE-RNP in TAS with 10% DMSO. Numbers indicate molar excess of the CBB compound versus ABE. g Fluorescence quantification of substrate and products of deamination and EndoV cleavage. Two independent samples per data point were assayed in parallel. Source data are provided as a Source Data file.
T7 Endonuclease Assay, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a Schematic diagram of the color-switching construct of the rd12 reporter-cell line. An intervening sequence from the Rpe65 - rd12 genomic sequence containing the mutation (c.130 C  >  T; p.R44X) was inserted into a gene expressing both mCherry and eGFP. Expression of eGFP in the reporter cells is prevented by a STOP codon, which can be restored via proper action of ABE. b Representative fluorescence-microscopic images of rd12 -reporter cells 48 h after adding the RNP-CBB complex. Scale bar, 400 μm. c , d Quantitative assessment of ABE-RNP delivery into rd12 -reporter cells, mediated by lipidoid complexes of ABE8e with CBB compounds ( c ) by flow cytometry; or CBBZ compounds ( d ) by fluorescence-microscopic imaging only. [ABE8e]/[CBB(Z)] = 1:8. A, ABE8e-RNP only. NE, no enzyme. Quantification of ABE8e RNP delivery mediated by Lipofectamine 3000 (LF) is included as a positive control. The ratio (eGFP/mCherry) reflects the relative editing efficiency. The CBB compounds were considered positive hits if the ratio of green-to-red (eGFP/mCherry) was more than twice that of A (the dashed line). Data are presented as mean ± SD, n = 4 replicates ( c ) and 3 replicates ( d ). e Schematic diagram of the ABE-activity assay. EndoV = endonuclease V. Blue, SpCas9 PAM; red, target base; orange, bystander base; arrowhead, nick site; FAM, fluorescein. f Representative urea-PAGE-gel image of products of ABE deamination cleaved by EndoV. The gel was imaged with a fluorescein filter. N, no enzyme; P, positive control: 60 bp DNA with inosine in the middle; A, ABE-RNP in TAS; D, ABE-RNP in TAS with 10% DMSO. Numbers indicate molar excess of the CBB compound versus ABE. g Fluorescence quantification of substrate and products of deamination and EndoV cleavage. Two independent samples per data point were assayed in parallel. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: A combinatorial synthetic strategy for developing genome-editing protein-delivery agents targeting mouse retina

doi: 10.1038/s41467-026-69077-w

Figure Lengend Snippet: a Schematic diagram of the color-switching construct of the rd12 reporter-cell line. An intervening sequence from the Rpe65 - rd12 genomic sequence containing the mutation (c.130 C  >  T; p.R44X) was inserted into a gene expressing both mCherry and eGFP. Expression of eGFP in the reporter cells is prevented by a STOP codon, which can be restored via proper action of ABE. b Representative fluorescence-microscopic images of rd12 -reporter cells 48 h after adding the RNP-CBB complex. Scale bar, 400 μm. c , d Quantitative assessment of ABE-RNP delivery into rd12 -reporter cells, mediated by lipidoid complexes of ABE8e with CBB compounds ( c ) by flow cytometry; or CBBZ compounds ( d ) by fluorescence-microscopic imaging only. [ABE8e]/[CBB(Z)] = 1:8. A, ABE8e-RNP only. NE, no enzyme. Quantification of ABE8e RNP delivery mediated by Lipofectamine 3000 (LF) is included as a positive control. The ratio (eGFP/mCherry) reflects the relative editing efficiency. The CBB compounds were considered positive hits if the ratio of green-to-red (eGFP/mCherry) was more than twice that of A (the dashed line). Data are presented as mean ± SD, n = 4 replicates ( c ) and 3 replicates ( d ). e Schematic diagram of the ABE-activity assay. EndoV = endonuclease V. Blue, SpCas9 PAM; red, target base; orange, bystander base; arrowhead, nick site; FAM, fluorescein. f Representative urea-PAGE-gel image of products of ABE deamination cleaved by EndoV. The gel was imaged with a fluorescein filter. N, no enzyme; P, positive control: 60 bp DNA with inosine in the middle; A, ABE-RNP in TAS; D, ABE-RNP in TAS with 10% DMSO. Numbers indicate molar excess of the CBB compound versus ABE. g Fluorescence quantification of substrate and products of deamination and EndoV cleavage. Two independent samples per data point were assayed in parallel. Source data are provided as a Source Data file.

Article Snippet: Purified DNA was nicked with 5 units of Endonuclease V (EndoV, NEB) for 2-3 h at 37 °C, after which the reaction was quenched by addition of TriTrack DNA-loading dye (1 × final) (Thermo Fisher, R1161) and incubation at 95 °C for 2 min.

Techniques: Construct, Sequencing, Mutagenesis, Expressing, Fluorescence, Flow Cytometry, Imaging, Positive Control, Activity Assay