Journal: Molecular Therapy. Nucleic Acids

Article Title: ac4C acetylation of RUNX2 catalyzed by NAT10 spurs osteogenesis of BMSCs and prevents ovariectomy-induced bone loss

doi: 10.1016/j.omtn.2021.06.022

Figure Lengend Snippet: Overexpression of NAT10 prevents bone loss in OVX mice (A) Illustration of adenoviruses overexpressing NAT10 or empty vector for OVX mice or sham mice, n = 6. (B and C) Confirmation of the efficiency of adenoviruses overexpressing NAT10 by western blotting. (C) Quantification of band intensities (B), n = 6. (D) The ac4C content of total RNA was increased in adenoviruses overexpressing NAT10 group compared with empty vector adenoviruses group, as determined by dot blot, n = 6. (E) Densitometry quantitation of (D), n = 6. (F) Representative μCT images of four groups of mice, n = 6. (G–K) Quantification of bone microstructural parameters of BV/TV (G), Tb.N (H), Tb.Th (I), Tb.Sp (J), and BSA/BV (K), n = 6. (L and M) OVX mice treated with adenoviruses overexpressing NAT10 demonstrated aggravated bone loss, but the bone mass of sham mice treated with Remodelin had no difference from sham mice treated with empty vector adenoviruses (scale bars: 100 μm), as determined by H&E staining, n = 6. (N) Quantification of the trabecular area from four groups of mice, n = 6. All data are expressed as the means ± SD. ∗p < 0.05, ∗∗p < 0.01 (n = 3 independent experiments).

Article Snippet: NAT10 overexpression adenovirus (NAT10-OE) and empty vector adenovirus (NC-OE) were constructed by OBiO Technology (Shanghai, China).

Techniques: Over Expression, Plasmid Preparation, Western Blot, Dot Blot, Quantitation Assay, Staining